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EasySep™FITC正选试剂盒II

对来源于单细胞悬液中标记 FITC 缀合抗体的细胞进行免疫磁珠正选分离

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产品号 #（选择产品）

产品号 #17682_C

免疫磁珠细胞正选试剂盒

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™FITC正选试剂盒II（产品号 #17682）
    • EasySep™FITC分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
  • RoboSep™ FITC正选试剂盒II（产品号  #17681RF）
    • EasySep™ FITC分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）x 2
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  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
产品说明书及文档
相关材料与文献
相关产品

总览

使用EasySep™ FITC正选试剂盒II，通过免疫磁性正选法可从任何单细胞悬液中分离经异硫氰酸荧光素（FITC）偶联抗体标记的高纯度细胞。EasySep™技术已在超过20年的公开研究中得到广泛应用，结合单克隆抗体特异性与无柱磁系统简便性。在此正选步骤中，目标细胞与能够识别FITC的和磁珠的抗体复合物结合。标记细胞通过EasySep™磁体分离后，未标记细胞被去除，目标细胞保留在试管中。分离完成后，目标细胞可直接用于流式细胞术、细胞培养或细胞检测。

本产品取代旧版EasySep™ FITC正选试剂盒（产品号#18558），实现更快速的细胞分离。

了解更多EasySep™免疫磁性技术或RoboSep™自动化系统。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其他物种，血浆，T 细胞
 
种属
非人灵长类，其他物种，大鼠
 
样本来源
骨髓，白膜层，脐带血，白细胞单采术样本，其他物种，PBMC，外周血，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English

相关材料与文献

技术资料 (6)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

文献 (1)

Innate lymphoid cells: NK and cytotoxic ILC3 subsets infiltrate metastatic breast cancer lymph nodes. L. Rethacker et al. Oncoimmunology 2022

Abstract

Innate lymphoid cells (ILCs) - which include cytotoxic Natural Killer (NK) cells and helper-type ILC - are important regulators of tissue immune homeostasis, with possible roles in tumor surveillance. We analyzed ILC and their functionality in human lymph nodes (LN). In LN, NK cells and ILC3 were the prominent subpopulations. Among the ILC3s, we identified a CD56+/ILC3 subset with a phenotype close to ILC3 but also expressing cytotoxicity genes shared with NK. In tumor-draining LNs (TD-LNs) and tumor samples from breast cancer (BC) patients, NK cells were prominent, and proportions of ILC3 subsets were low. In tumors and TD-LN, NK cells display reduced levels of NCR (Natural cytotoxicity receptors), despite high transcript levels and included a small subset CD127- CD56- NK cells with reduced function. Activated by cytokines CD56+/ILC3 cells from donor and patients LN acquired cytotoxic capacity and produced IFNg. In TD-LN, all cytokine activated ILC populations produced TNF$\alpha$ in response to BC cell line. Analyses of cytotoxic and helper ILC indicate a switch toward NK cells in TD-LN. The local tumor microenvironment inhibited NK cell functions through downregulation of NCR, but cytokine stimulation restored their functionality.
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更多信息

更多信息
物种 其它物种, 大鼠, 非人灵长类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 PBMC, 其它细胞系, 外周血, 白细胞单采术样本, 白膜层, 脐带血, 脾脏, 骨髓
Selection Method Positive
法律声明:

Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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