实验室基础知识
无论您是实验室新手还是需要更新知识,拥有有关基础研究技术的资源可供参考都会有所帮助。 从这里开始了解细胞培养、基本细胞分选的方法、基础的细胞测定、或其他关键的分子生物学技术。
实验方案
- 视频 How to Thaw Human iPSC Lines
- 视频 Freeze Human Primary Cells with CryoStor® CS10
- 技术窍门 Aggregate Passaging for ES and iPS Cell Maintenance
- 实验方案 How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture
- 实验方案 How to Coat Cultureware with Vitronectin XF™ for Pluripotent Stem Cell Culture
- 技术窍门 Recommendations for Coating Plates with Matrigel® for Pluripotent Stem Cell Culture
- 技术窍门 Using Vitronectin XF™ Defined Matrix for Human Pluripotent Stem Cells
- 实验方案 How to Thaw Frozen Primary Cells
- 实验方案 How to Create a Hypoxic Environment for Cell Culture
- 实验方案 How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
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- 视频 How to Resuspend Magnetically Labeled Cells After EasySep™ Separation
- 视频 How to Aspirate an EasyPlate™ Isolation
- 视频 How to Correctly Pour Liquid from SepMate™ Tubes
- 视频 How to Prep Flask-Based Samples for EasySep™
- 实验方案 How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
- 实验方案 How to Reduce Cell Clumping in Single Cell Suspensions
- 实验方案 How to Remove Granulocytes from Old Blood Samples
- 实验方案 How to Generate a Buffy Coat
- 技术窍门 Centrifugation Speed and Time for Cells
- 技术窍门 Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures
- 技术窍门 Reducing Platelet Contamination
- 技术窍门 Choosing a Negative or Positive Selection Strategy
- 技术窍门 Comparing Cell Separation Platforms from STEMCELL Technologies
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- 实验方案 How to Count Cells with a Hemocytometer
- 实验方案 How to Count Cell Spheres in Suspension Culture
- 技术窍门 Total Nucleated Cell and Viable Cell Counts
- 视频 Procedure for Setting Up the CFU Assay
- 技术窍门 Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage
- 实验方案 How to Characterize Extracellular Vesicles by Western Blotting
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