EasySep™ Biotin正选试剂盒

通过生物素抗体标记细胞的免疫磁珠细胞正选试剂盒

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产品号 #（选择产品）

产品号 #17683_C

通过生物素抗体标记细胞的免疫磁珠细胞正选试剂盒

产品优势

快速、简单
无需分离柱

产品组分包括

EasySep™ Biotin正选试剂盒II（产品号＃17683）

EasySep™ Biotin分选抗体混合物，1 mL
EasySep™ Dextran RapidSpheres™磁珠，1 mL
RoboSep™试管，用于一抗偶联抗体（手动使用时无需此项），1 vial

RoboSep™ Biotin正选试剂盒II（产品号＃17683RF）

EasySep™Biotin分选抗体混合物，1 mL
EasySep™ Dextran RapidSpheres™磁珠，1 mL
RoboSep™试管，用于一抗偶联抗体（手动使用时无需此项），1 vial
RoboSep™缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）x 2

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总览

使用EasySep™生物素正选试剂盒 II，通过免疫磁珠正选技术从任何单细胞悬液样本中分离出经生物素化抗体标记的高纯度细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁珠分选系统的简便性，已在发表的研究中广泛应用超过20年。

该EasySep™ 正选流程中，目的细胞被识别生物素和葡聚糖包被的磁珠抗体复合物标记。标记细胞通过EasySep™磁极被分选，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。完成磁珠细胞分选后，目标生物素阳性细胞即可用于流式细胞术、细胞培养或细胞实验等下游应用。

本产品替代EasySep™ Biotin正选试剂盒（产品号#17683）以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选实现全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加物、抗体等。

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

文献 (2)

C5aR1 inhibition reprograms tumor associated macrophages and reverses PARP inhibitor resistance in breast cancer Nature Communications 2024 May

Abstract

Although Poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) have been approved in multiple diseases, including BRCA1/2 mutant breast cancer, responses are usually transient requiring the deployment of combination therapies for optimal efficacy. Here we thus explore mechanisms underlying sensitivity and resistance to PARPi using two intrinsically PARPi sensitive (T22) and resistant (T127) syngeneic murine breast cancer models in female mice. We demonstrate that tumor associated macrophages (TAM) potentially contribute to the differential sensitivity to PARPi. By single-cell RNA-sequencing, we identify a TAM_C3 cluster, expressing genes implicated in anti-inflammatory activity, that is enriched in PARPi resistant T127 tumors and markedly decreased by PARPi in T22 tumors. Rps19/C5aR1 signaling is selectively elevated in TAM_C3. C5aR1 inhibition or transferring C5aR1hi cells increases and decreases PARPi sensitivity, respectively. High C5aR1 levels in human breast cancers are associated with poor responses to immune checkpoint blockade. Thus, targeting C5aR1 may selectively deplete pro-tumoral macrophages and engender sensitivity to PARPi and potentially other therapies. PARP inhibitors (PARPi) have been approved for the treatment of metastatic triple-negative breast cancer (BC), however resistance and recurrence are often observed. Here, in preclinical models of BRCA1/2 wild type and homologous recombination competent BC, the authors show that C5aR1-positive tumor associated macrophages are associated with PARPi-resistance, suggesting targeting C5aR1 as a therapeutic option.

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Thymocyte Maturation and Emigration in Adult Mice. K. Joannou et al. Journal of immunology (Baltimore, Md. : 1950) 2022 may

Abstract

Several unique waves of ?? T cells are generated solely in the fetal/neonatal thymus, whereas additional ?? T cell subsets are generated in adults. One intriguing feature of ?? T cell development is the coordination of differentiation and acquisition of effector function within the fetal thymus; however, it is less clear whether this paradigm holds true in adult animals. In this study, we investigated the relationship between maturation and thymic export of adult-derived ?? thymocytes in mice. In the Rag2pGFP model, immature (CD24+) ?? thymocytes expressed high levels of GFP whereas only a minority of mature (CD24-) ?? thymocytes were GFP+ Similarly, most peripheral GFP+ ?? T cells were immature. Analysis of ?? recent thymic emigrants (RTEs) indicated that most ?? T cell RTEs were CD24+ and GFP+, and adoptive transfer experiments demonstrated that immature ?? thymocytes can mature outside the thymus. Mature ?? T cells largely did not recirculate to the thymus from the periphery; rather, a population of mature ?? thymocytes that produced IFN-? or IL-17 remained resident in the thymus for at least 60 d. These data support the existence of two populations of ?? T cell RTEs in adult mice: a majority subset that is immature and matures in the periphery after thymic emigration, and a minority subset that completes maturation within the thymus prior to emigration. Additionally, we identified a heterogeneous population of resident ?? thymocytes of unknown functional importance. Collectively, these data shed light on the generation of the ?? T cell compartment in adult mice.

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物种	其它物种, 大鼠, 非人灵长类
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	PBMC, 其它细胞系, 外周血, 白细胞单采术样本, 白膜层, 脐带血, 脾脏, 骨髓
Selection Method	Positive

EasySep™ Biotin正选试剂盒

产品号 #17683

产品号 #17683RF

产品号 #（选择产品）

产品号 #17683_C

产品优势

产品组分包括

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产品说明书及文档

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EasySep™ Biotin正选试剂盒

产品号 #17683

产品号 #17683RF

产品号 #（选择产品）

产品号 #17683_C

产品优势

产品组分包括

总览

产品说明书及文档

相关材料与文献

技术资料 (6)

文献 (2)

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Abstract

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