人iPSC衍生的神经祖细胞

冻存的人神经祖细胞由人诱导多能干细胞系SCTi003-A分化而来

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产品号 #200-0620_C

冻存的人神经祖细胞由人诱导多能干细胞系SCTi003-A分化而来

产品优势

复苏后可立即用STEMdiff™神经祖细胞培养基扩增
节省时间，使用高度鉴定的神经祖细胞中间体开始您的分化工作流程
可使用STEMdiff™神经系统分化为前脑神经元和/或星形胶质细胞
确保与相同遗传背景下产生的神经元-星形胶质细胞共培养物具有生理相关性
获得高品质的NPCs，其来源于高度鉴定的对照细胞系SCTi003-A

产品组分包括

人iPSC衍生的神经祖细胞（产品号 #200-0620）

立即询价

总览

实验数据

产品说明书及文档

应用领域

总览

使用高品质、即用型人神经祖细胞 (NPCs)，自信地开始您的神经研究工作流程。这些特殊冻存的中枢神经系统 (CNS) 型祖细胞由人诱导多能干细胞 (iPSC) 对照系SCTi003-A分化而来，该细胞系源自健康女性供体的外周血单分核细胞 (PBMCs)。这些人NPCs复苏后可直接使用，且具有多能性，适用于使用STEMdiff™试剂盒使其下游定制化地分化为各种中枢神经系统细胞类型，如前脑神经元, 中脑神经元,星形胶质细胞。

为了确保细胞的高质量，这些NPCs通过使用无血清的STEMdiff™SMADi神经诱导试剂盒从稳定且经过广泛测试的SCTi003-A系分化而来。NPCs可以使用STEMdiff™神经祖细胞培养基进行扩增，从而实现规模化生产并降低需要大量细胞的工作流程的成本。使用STEMdiff™神经祖细胞冻存液冻存扩增的神经祖细胞 (NPCs)，为您的实验计划提供灵活性。

本产品仅供研究使用 (RUO)，已获得学术和商业用途许可。血液样本的采集符合伦理，并遵循机构审查委员会 (IRB) 或其他监管机构批准的知情同意书和方案。有关供体详情和来源细胞库的细胞质量鉴定，请参阅本页的数据图。SCTi003-A源自αβ T细胞，并经过VDJ序列重排。SCTi003-A核型稳定，具有三谱系分化潜能，表达未分化的细胞标志物，并使用非整合重编程技术进行重编程。已在hPSCreg®注册以确保符合基于社会标准的伦理和生物学合规性。如需更多细节，请参阅特定批次的分析证书和关于iPSC细胞系的常见问题解答。

分类
冻存

包含
STEMdiff™神经祖细胞冻存液

细胞类型
神经干/祖细胞

种属
人

细胞和组织来源
多能干细胞

应用
细胞培养，功能学筛选

品牌
STEMdiff

研究领域
疾病建模，药物发现和毒理检测，神经科学

供体状态
正常

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实验数据

Thawed and plated Human iPSC-Derived Neural Progenitor Cells exhibit high-quality, characteristic NPC morphology.

Figure 1. Human iPSC-Derived Neural Progenitor Cells Exhibit High-Quality Morphology Characteristic of Multipotent Central Nervous System Progenitor Cells

Cryopreserved Human iPSC-Derived Neural Progenitor Cells were thawed and plated onto Corning® Matrigel®-coated plates at 200,000 cells/cm². NPCs were incubated for 24 hours in STEMdiff™ Neural Progenitor Medium at 37℃ and subsequently analyzed by brightfield microscopy. NPCs display the small, teardrop-shaped morphology expected for NPCs. (A) 4X magnification, (B) 10X magnification. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells expressing neural progenitor markers SOX1 and PAX6, with low βIII-TUB expression.

Figure 2. Human iPSC-Derived Neural Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Neural Progenitor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. The NPCs express neural progenitor markers (A) SOX1 and (B) PAX6 with low expression of (C) βIII-TUB. (D) In addition, they display the expected small, teardrop-shaped morphology. (E) The percentage expression of these markers were quantified. Neural progenitor markers PAX6 and SOX1 were found to be expressed in 90% of NPCs, while mature neuronal marker βIII-TUB was expressed in less than 10% of NPCs. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to forebrain neurons expressing neural identity marker βIII-TUB.

Figure 3. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Forebrain Neurons

Forebrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Forebrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Forebrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Forebrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting forebrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (magenta), (C) but not astrocyte marker GFAP (green). (D) Nuclei are labeled with DAPI (gray). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to midbrain neurons expressing neural identity marker, βIII-TUB, and dopaminergic neuron marker, TH, but not astrocyte marker, GFAP.

Figure 4. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Midbrain Neurons

Midbrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Midbrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Midbrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Midbrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting midbrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (red) and (C) dopaminergic neuron marker TH (green), (D) but not astrocyte marker GFAP (magenta). (D) Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to astrocytes expressing astrocyte identity marker S100β and GFAP, but not neuronal marker DCX.

Figure 5. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Astrocytes

Astrocytes were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Astrocyte Differentiation Medium. NPCs were cultured for 21 days at 37℃ in STEMdiff™ Astrocyte Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Astrocyte Maturation medium for seven days. Resulting cells were processed for immunocytochemistry. (A) The resulting astrocyte cultures contain a population of cells expressing (B) astrocyte marker S100β (green) and (C) GFAP (red), but not neuronal marker DCX (magenta). Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Bar graph showing expansion rate of Human iPSC-Derived Neural Progenitor Cells to 5 passages.

Figure 6. Human iPSC-Derived Neural Progenitor Cells Can Be Expanded for Multiple Passages

Human iPSC-Derived Neural Progenitor Cells were thawed and maintained in STEMdiff™ Neural Progenitor Medium at 37℃ and passaged roughly once every seven days for a total of five weeks. The average fold increase of NPCs was 2.8 ± 0.5 (mean ± SEM) per passage, demonstrating cells can be effectively expanded.