健康对照人iPSC细胞系，女性，SCTi005-A

冻存的人多能干细胞系

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产品号 #（选择产品）

产品号 #200-0944_C

冻存的人多能干细胞系

产品优势

通过机构审查委员会 (IRB) 的方案进行收集，确保符合伦理规范，已获得学术和商业使用授权。
严格遵循国际标准（ISSCR 2023指南与ISCBI 2009规范）的全流程质控体系。
通过hPSCreg®认证的细胞系，确保研究透明度与生物伦理合规性。
无缝对接标准化培养体系：TeSR™培养基（维持培养）与STEMdiff™分化试剂。

产品组分包括

健康对照人iPSC细胞系，女性，SCTi005-A（产品号 #200-0944）

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

ReLeSR™
mTeSR™ Plus

总览

实验数据

产品说明书及文档

总览

使用SCTi005-A细胞系来源的高质量诱导多能干细胞 (iPSC) ，助您轻松开启您的研究。SCTi005-A来源于健康女性供体外周血单个核细胞（PBMC），适用于多种应用，如正常对照，基因编辑或定向分化为特定谱系细胞及类器官。

为确保产品性能与实验可重复性，SCTi005-A细胞系采用包含mTeSR™ Plus，Corning® Matrigel® hESC专用基质，以及ReLeSR™解离试剂的培养体系生产，并严格遵循相关质控标准。SCTi005-A核型稳定，具有三胚层分化潜能，高表达未分化细胞标志物，通过非整合性重编程技术获得。已在hPSCreg®注册，符合国际干细胞研究学会（ISSCR）的伦理与生物学标准。

选择TeSR™和STEMdiff™细胞培养基产品，为您的细胞培养系统建立完整工作流程。如需其他基因型的iPSC对照细胞系，请参阅我们全系列高质量健康对照人iPSC细胞系（含女性及男性供体来源）。

本产品仅供研究使用 (RUO) ，已获得学术和商业使用授权。原始血样采集均通过机构审查委员会 (IRB) 或其他监管机构批准的知情同意书和方案进行，确保符合伦理规范。源细胞库的供体详细信息和细胞质量表征数据请参阅本页面图表。更多详情请参考批次对应的 COA 以及 iPSC细胞系常见问题解答。

全外显子组及全基因组测序数据文件可根据需求提供，若有需求请联系我们。

某些产品仅在特定地区出售。请与您的销售代表或产品与科学支持联系techsupport@stemcell.com获取更多信息。

成分说明
CryoStor® CS10

分类
冻存

细胞类型
多能干细胞，健康对照

物种
人

细胞与组织来源
外周血，多能干细胞

研究领域
细胞系开发，疾病建模，药物发现与毒理检测，传染病，神经科学，类器官，干细胞生物学

供者状态
正常

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实验数据

Table 1. iPSC Line SCTi005-A Is Derived from a Healthy Female Donor

Demographic, health, and genetic characteristics of the SCTi005-A donor are compiled based on self-reported information and whole-exome sequencing. Sex was determined by karyotype. Ancestry and HLA haplotype were calculated from whole-genome and whole-exome sequencing combined data. Blood type (ABO/Rh blood group) was determined by next-generation sequencing. Height, weight, and BMI were calculated at the donation facility. iPSC = induced pluripotent stem cell.

Figure 1. SCTi005-A Human Pluripotent Stem Cells Demonstrate High-Quality Morphology in Routine Culture

Cryopreserved cells from line SCTi005-A were thawed and maintained in mTeSR™ Plus on Corning® Matrigel® Matrix.

(A) The resulting iPSC colonies have densely packed cells and show multi-layering when ready to be passaged.

(B,C) Cells retain prominent nucleoli and high nuclear-to-cytoplasmic ratios. iPSC = induced pluripotent stem cell.

Figure 2. SCTi005-A Human Pluripotent Stem Cells Maintain a Normal Karyotype

(A) G-T-L banding for thawed cells at p12 (n = 20) shows a normal karyotype with no evidence of clonal abnormalities at a band resolution of 425 - 500 G-bands per haploid genome.

(B) Fluorescent in situ hybridization in a representative p12 iPSC using probes for 20p11 (green) and 20q11.21 (red). 95% of cells examined displayed two sets of two probe signals, indicating no aneusomy of chromosome 20 (n = 200). iPSC = induced pluripotent stem cell.

Table 2. Single Nucleotide Polymorphism Microarray Analysis Characterizes SCTi005-A Copy Number Variants

DNA was extracted from a vial of SCTi005-A from the Master Cell Bank and subject to SNP microarray analysis to identify large-scale copy number variants (CNVs). The cells display no reportable CNVs, defined as those greater than 400kb in size. Array design, genomics position, genes, and chromosome banding are based on genome build GRCh38/hg3. chr = chromosome; bp = base pairs; SNP = single nucleotide polymorphism.

Figure 3. SCTi005-A iPSCs Express Undifferentiated Cell Markers

Cell line SCTi005-A was characterized using flow cytometry for undifferentiated cell markers OCT3/4 and TRA-1-60.

(A) Percentage marker expression was quantified 3 passages after thawing from the analyses of two biological replicates.

Representative flow cytometry plots are displayed for (B) OCT3/4 and (C) TRA-1-60. iPSC = induced pluripotent stem cell.

Figure 4. SCTi005-A Human Pluripotent Stem Cells Demonstrate a High Trilineage Differentiation Capacity

Cells from SCTi005-A were split into 3 groups, differentiated using STEMdiff™ Trilineage Differentiation Kit (Catalog #05230), and then subjected to flow cytometry analysis. Two markers for each embryonic germ layer were assessed, and bars present mean marker expression for each group of cells (n = 2 biological replicates). PAX6 and Nestin confirm differentiation to the ectoderm lineage, NCAM and Brachyury (T) to the mesoderm lineage, and CXCR4 and SOX17 to the endoderm lineage.

Table 3. Comprehensive Risk Variant Profile for the SCTi005-A iPSC Line

The table presents risk variants identified for the SCTi005-A iPSC line. DNA was purified from the master cell bank, and whole-genome sequencing was performed to capture the entire genomic landscape. Sequencing was conducted on the NovaSeq 6000 System, achieving a 50x coverage. Coverage of coding regions and adjacent splice junction sites was enhanced for 20,000 genes using the SureSelect Human All Exon V6 Kit (Agilent Technologies). Variants were called and analyzed following GATK4 best practices, incorporating a Convolutional Neural Network to score each variant. High-confidence variants were annotated with predicted functional consequences and ClinVar IDs. ClinVar entries required two or more supporting ALT reads and an assertion criteria of one gold star or more in ClinVar to be considered. This comprehensive profile assists in understanding the genetic landscape and potential health impacts associated with the SCTi005-A iPSC line.