In this series of three Technical Videos, we will guide you through the protocol for transitioning human pluripotent stem cells (or hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This series of technical videos will help ensure a successful transition of your hPSC lines into mTeSR™ Plus. We will use mTeSR™1 cultures transitioning to mTeSR™ Plus as an example for this video. If other feeder-free media are being used, some alterations to this protocol may be necessary. Video 1: Plate Coating Video 2: Generation of Cell Aggregates/Passaging Video 3: Maintenance & Morphological Assessment Learn more about mTeSR™ Plus hPSC maintenance media > View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.