In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1, into mTeSR™ Plus.

This video will cover maintenance and morphological assessment of high-quality hPSCs cultured in mTeSR™ Plus.

Key topics covered in this video:

• • Differences in hPSCs cultured in mTeSR™1 or mTeSR™ Plus
• • How to assess quality of hPSC cultures through morphology using phase-contrast microscopy
• • Tips to recognize and monitor spontaneous differentiation

Watch the other two videos in the series below:

Video 1: Plate Coating for hPSC Cultures
Video 2: How to Generate and Passage hPSC Aggregates

Learn more about mTeSR™ Plus hPSC maintenance media >