EasySep™人ILC2分选试剂盒

采用磁解离放技术，对来源于外周血单个核细胞或洗涤后白细胞样本中的 ILC2（CRTH2⁺）进行免疫磁珠正选分离

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¥15,044.00

产品号 #（选择产品）

产品号 #17782_C

免疫磁珠负选细胞分选试剂盒

产品优势

快速、易于操作，且无需分离柱
纯度高达95%
无需流式分选即可快速分离ILC2
无需清洗去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

EasySep™人ILC2分选试剂盒 (产品号 #17782)

EasySep™人CRTH2-PE正选抗体混合物, 0.1 mL
EasySep™ Release PE正选抗体混合物, 1 mL
EasySep™ Releasable RapidSpheres™ 50201磁珠, 1 mL
EasySep™ Release缓冲液, 1 mL
EasySep™ 人ILC2细胞分选抗体混合物, 0.5 mL
EasySep™ Dextran RapidSpheres™ 50103磁珠, 1 mL
抗人CD32阻断剂, 1 mL

立即询价

总览

使用EasySep™人ILC2分离试剂盒，通过免疫磁性正选法可从新鲜外周血单个核细胞（PBMCs）或白细胞单采样品中分离高纯度、无磁珠的人第二型固有淋巴细胞（ILC2）。

该方法使用识别CRTH2的抗体复合物与可解离磁珠RapidSpheres™。与传统磁珠不同，RapidSpheres™可在分离后去除。目标细胞标记后经EasySep™磁体分离，未标记细胞被倒出，目标细胞保留在试管中，并使用解离试剂去除磁珠颗粒。所得CRTH2+细胞可立即用于流式细胞术、培养或其他下游应用。

Data Figures

Figure 1. Typical EasySep™ Human ILC2 Isolation Profile

Starting with fresh leukapheresis samples, the ILC2 content (CD45+Lin- CRTH2+CD161+CD127+) of the isolated fraction typically ranges from 84 - 95%. In the above example, the purities of the start and final isolated fractions are 0.03% and 91% (gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

1000063935 or higher

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 4

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 5

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 6

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Document Type

Safety Data Sheet 7

Product Name

EasySep™ Human ILC2 Isolation Kit

Catalog #

17782

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
物种	人类
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001)
样本来源	PBMC, 白细胞单采术样本
Selection Method	Positive