EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #17972_C
免疫磁珠负选细胞富集试剂盒
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总览
使用 EasySep™ 人 ILC2 富集试剂盒，通过免疫磁珠负选，可轻松高效地从洗涤的白细胞单采样本中分离高纯度的人 2 型固有淋巴样细胞（ILC2）。EasySep™ 技术结合单克隆抗体的特异性与无柱磁系统的简便性，已在发表的研究中广泛应用超过 20 年。
在此 EasySep™ 负选过程中，不需要的细胞通过抗体复合物和磁珠标记。表达以下标记的细胞将被去除：CD2、CD3、CD4、CD14、CD16、CD19、CD24、CD34、CD56、CD61、CD66b、CD123 和 GlyA。使用 EasySep™ 磁极进行无柱分选，磁珠标记的细胞被去除，只需将未标记的目的ILC2 细胞倒入或移取至新的管中即可。完成磁珠分选后，目的 ILC2 细胞可直接用于下游应用，如流式细胞术和细胞分选。
了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。
磁极兼容性
• “The Big Easy” EasySep™磁极（产品号 #18001）
• Easy 50 EasySep™磁极（产品号 #18002）
分类
细胞分选试剂盒
细胞类型
先天性淋巴细胞
种属
人
样本来源
白细胞单采术样本
分选方法
负选
应用
细胞分选
品牌
EasySep
研究领域
免疫
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (3)
文献 (1)
Impact of innate lymphoid cell type 2 in chronic lymphocytic leukemia on the function of treg and CD8+ T cells through IL-9
Cancer Immunology, Immunotherapy : CII 2025 May
Abstract
ObjectiveThis study investigated the impact of innate lymphoid cell type 2 (ILC2s) on the function of regulatory T cells (Treg) and CD8+ T cells in chronic lymphocytic leukemia (CLL) through IL-9.MethodsPeripheral blood samples were collected from CLL patients (n = 52) and healthy controls (n = 30). ILC2 proportions and IL-9 levels were assessed using flow cytometry and ELISA. Immunofluorescence staining was performed to stain GATA3, CRTH2, and IL-9 in cervical lymph nodes from CLL patients (n = 10) and control subjects with reactive lymphadenitis (n = 10). Correlation analysis between ILC2s and IL-9 was conducted using the Spearman test. ILC2s were sorted and cultured from CLL patients, followed by co-culture experiments with PBMCs of healthy controls and MEC-1 cells, with or without anti-IL-9 antibody intervention. Flow cytometry was used to measure the proportions of ILC2s, Treg cells, PD-1+/TIGIT+/CTLA-4+ Treg subsets, and granzyme B+/perforin+ CD8+ T cells, along with MEC-1 cell apoptosis.ResultsThe proportions of ILC2s and Treg, along with serum IL-9 levels, were significantly elevated in CLL patients (P < 0.05). Peripheral blood ILC2s were positively correlated with IL-9 (r = 0.609, P < 0.001). The average fluorescence intensity of GATA3, CRTH2, and IL-9 in the cervical lymph nodes of CLL patients increased significantly (P < 0.001), and IL-9 showed colocalization with GATA3 and CRTH2. In vitro, IL-9 levels in the supernatant of sorted ILC2s from CLL patients increased. Treatment with anti-IL-9 antibody significantly reduced the PD-1+ Treg and TIGIT+ Treg cells while increasing granzyme B+ CD8+ T cells (P < 0.05). However, there was no significant effect on Treg, CTLA-4+ Treg, and perforin+ CD8+ T cells (P > 0.05). Additionally, anti-IL-9 antibody significantly increased early apoptosis (P < 0.05).ConclusionILC2s affect CD8+ T cells and Treg cells through IL-9, weakening the anti-tumor effects of CD8+ T cells and enhancing the immunosuppressive effects of Treg cells, thereby contributing to CLL pathogenesis.Supplementary InformationThe online version contains supplementary material available at 10.1007/s00262-025-04082-4.
更多信息
| 物种 | 人类 |
|---|---|
| Magnet Compatibility | • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) |
| 样本来源 | 白细胞单采术样本 |
| Selection Method | Negative |
相关产品
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EasySep™人Pan-ILC富集试剂盒<p>对来源于洗涤的白细胞单采术样本的未标记的 ILC1、ILC2、ILC3 细胞进行免疫磁珠负选分离</p>
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Easy50 EasySep™磁极<p>无柱免疫磁珠分选磁极</p>
标记抗体
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抗人CD14抗体，clone M5E2抗人、恒河猴、食蟹猴CD14的小鼠Monoclonal IgG2a抗体
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抗人CD16抗体，clone 3G8小鼠抗人、恒河猴、食蟹猴CD16 Monoclonal IgG1抗体
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抗人CD19抗体，clone HIB19抗人、黑猩猩CD19的小鼠Monoclonal IgG1抗体
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抗人CD3抗体，clone UCHT1小鼠（BALB/c）Monoclonal IgG1抗体，抗人、黑猩猩CD3
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抗人CD34抗体，clone 581小鼠Monoclonal IgG1抗体，抗人CD34
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抗人CD45抗体，clone HI30小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45
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抗人CD123（IL-3Rα）抗体，clone 6H6抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠Monoclonal IgG1抗体
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