EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #19842_C
免疫磁珠负选细胞分选试剂盒
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总览
使用 EasySep™ 小鼠第二类固有淋巴细胞（ILC2）富集试剂盒，可通过免疫磁性负选法从小鼠肺或其他组织样本的单细胞悬液中轻松高效地分离高纯度的小鼠 ILC2 细胞。EasySep™ 技术在已发表研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。
在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物和磁性颗粒进行标记。表达以下标志物的细胞将被去除：CD4、TCRγδ、TCRαβ、CD5、CD45R、Ter119、CD19、Ly6G/C、CD11b 和 CD11c。经 EasySep™ 磁体分离后，带有磁性标记的不需要细胞被去除，未标记的目标小鼠 ILC2 细胞通过倾倒或移液方式转移至新试管中。磁性细胞分离完成后，目标细胞可直接用于流式细胞术或细胞分选等下游应用。
了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。
磁极兼容性
• EasySep™磁极（产品号 #18000）
分类
细胞分选试剂盒
细胞类型
先天性淋巴细胞
种属
小鼠
样本来源
其他物种
分选方法
负选
应用
细胞分选
品牌
EasySep
研究领域
免疫
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
文献 (2)
Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC. STAR protocols 2022 sep
Abstract
Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
IL-33 induces NF-$\kappa$B activation in ILC2 that can be suppressed by in vivo and ex vivo 17$\beta$-estradiol. Frontiers in allergy 2022
Abstract
Asthmatic women tend to develop severe airway disease in their reproductive years, and 30%-40% of asthmatic women have peri-menstrual worsening of asthma symptoms. This indicates that fluctuations in ovarian hormones are involved in advancement of asthmatic disease and exacerbation of symptoms. Group 2 innate lymphoid cells, or ILC2, are readily detected in allergic conditions, such as rhinosinusitis, in individuals that develop nasal polyps do to allergen exposures, and in allergic asthma. ILC2 are airway localized immune cells activated by IL-33, an innate cytokine that perpetuates allergic inflammation by driving the production of IL-5 and IL-13. We have previously shown that ILC2 are highly activated in na{\{i}}ve and ovalbumin (OVA) challenged female BALB/c mice in comparison to male mice following stimulation with IL-33. Here we investigated the effect of steady-state ovarian hormones on ILC2 and the NF-$\kappa$B signaling pathway following OVA sensitization and challenge. We found that estrogen-treated ovariectomized mice (OVX-E2) that had been challenged with OVA had reduced IL-5 and IL-13 production by lung ILC2 as compared to lung ILC2 isolated from intact male and female sham-operated controls that had been treated with OVA. ILC2 were isolated from untreated animals and co-cultured ex vivo with and without estrogen plus IL-33. Those estrogen-treated ILC2 similarly produced less IL-5 and IL-13 in comparison to untreated and had reduced NF-$\kappa$B activation. Single-cell RNA sequencing showed that 120 genes were differentially expressed in male and female ILC2 and Nfkb1 was found among top-ranked regulatory interactions. Together these results provide new insight into the suppressive effect of estrogen on ILC2 which may be protective in female asthmatics. Understanding further how estrogen modulates ILC2 may provide therapeutic targets for the treatment of allergic diseases."
更多信息
| 物种 | 小鼠 |
|---|---|
| Magnet Compatibility | EasySep™ Magnet (Catalog #18000) |
| 样本来源 | 其它细胞系 |
| Selection Method | Negative |
标记抗体
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抗小鼠CD11c抗体，clone N418抗小鼠CD11c的仓鼠（亚美尼亚）Monoclonal IgG抗体
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抗小鼠CD19抗体，clone ID3大鼠Monoclonal IgG2a抗体，抗小鼠CD19
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抗小鼠CD3e抗体，clone 145-2C11亚美尼亚仓鼠Monoclonal IgG1抗体，抗小鼠CD3e
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抗小鼠CD45抗体，clone 30-F11大鼠Monoclonal IgG2b抗体，抗小鼠CD45
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抗小鼠Gr-1抗体，clone RB6-8C5大鼠Monoclonal IgG2b抗体，抗小鼠Gr-1 （Ly-6G/Ly-6C）
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抗小鼠NK1.1（CD161）抗体，clone PK136抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交Monoclonal IgG2a抗体
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抗小鼠TCR Gamma/Delta抗体，clone GL3亚美尼亚仓鼠Monoclonal IgG2抗体，抗小鼠T细胞受体 gamma/delta
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抗小鼠TER119抗体，clone TER-119抗小鼠TER119的大鼠（WI）Monoclonal IgG2b抗体
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抗小鼠CD11b抗体，clone M1/70抗人、小鼠、恒河猴CD11b的大鼠Monoclonal IgG2b抗体
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