EasySep™人Pan-ILC富集试剂盒

对来源于洗涤的白细胞单采术样本的未标记的 ILC1、ILC2、ILC3 细胞进行免疫磁珠负选分离

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产品号 #（选择产品）

产品号 #17975_C

免疫磁珠负选细胞富集试剂盒

产品优势

操作简单、快捷，且无需分离柱
获得的活细胞无标记
利于快速流式分选ILC

产品组分包括

EasySep™ 人Pan-ILC富集试剂盒（产品号 #17975）

EasySep™人Pan-ILC2富集抗体混合物，0.5 mL
EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL

RoboSep™ 人Pan-ILC富集试剂盒（产品号 #17975 RF）

EasySep™人Pan-ILC2富集抗体混合物，0.5 mL
EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
RoboSep™ 缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）

立即询价

总览

使用 EasySep™ 人全 ILC 富集试剂盒，通过免疫磁珠负选，可轻松高效地从洗涤的白细胞单采样本中分离高纯度的 1、2 和 3 型固有淋巴样细胞（ILC1、ILC2 和 ILC3）。EasySep™ 技术结合单克隆抗体的特异性与无柱磁系统的简便性，已在发表的研究中广泛应用超过 20 年。

在此 EasySep™ 负选过程中，不需要的细胞通过抗体复合物和磁珠标记。表达以下标记的细胞将被去除：CD3、CD4、CD11c、CD14、CD16、CD19、CD24、CD34、CD36、CD61、CD66b、CD94、CD123 和 GlyA。使用 EasySep™ 磁极进行无柱分选，磁珠标记的细胞被去除，只需将未标记的目的ILC 细胞倒入或移取至新的管中即可。完成磁珠分选后，目的 ILC 细胞可直接用于下游应用，如流式细胞术和细胞分选。

了解更多关于 EasySep™ 免疫磁性技术的工作原理，或了解如何使用 RoboSep™ 实现免疫磁性细胞分离的全自动化。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。

实验数据

Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

文献 (1)

Endothelin-A Receptor Antagonist Alleviates Allergic Airway Inflammation via the Inhibition of ILC2 Function. X. Zhang et al. Frontiers in immunology 2022

Abstract

Allergic airway inflammation is a universal airway disease that is driven by hyperresponsiveness to inhaled allergens. Group 2 innate lymphoid cells (ILC2s) produce copious amounts of type 2 cytokines, which lead to allergic airway inflammation. Here, we discovered that both peripheral blood of human and mouse lung ILC2s express the endothelin-A receptor (ETAR), and the expression level of ETAR was dramatically induced upon interleukin-33 (IL-33) treatment. Subsequently, both preventive and therapeutic effects of BQ123, an ETAR antagonist, on allergic airway inflammation were observed, which were associated with decreased proliferation and type 2 cytokine productions by ILC2s. Furthermore, ILC2s from BQ123 treatment were found to be functionally impaired in response to an interleukin IL-33 challenged. And BQ123 treatment also affected the phosphorylation level of the extracellular signal-regulated kinase (ERK), as well as the level of GATA binding protein 3 (GATA3) in activated ILC2s. Interestingly, after BQ123 treatment, both mouse and human ILC2s in vitro exhibited decreased function and downregulation of ERK signaling and GATA3 stability. These observations imply that ETAR is an important regulator of ILC2 function and may be involved in ILC2-driven pulmonary inflammation. Therefore, blocking ETAR may be a promising therapeutic strategy for allergic airway inflammation.

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更多信息

更多信息
物种	人类
Magnet Compatibility	• “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	PBMC, 白细胞单采术样本
Selection Method	Negative