MesenCult™-ACF Plus培养试剂盒

用于人间充质干细胞的无动物成分培养基及贴附基质

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产品号 #05448

用于人间充质干细胞的无动物成分培养基及贴附基质

产品优势

无动物成分配方提升实验可重复性。
相较于含血清培养基，具备更优异的细胞扩增效果。
培养的MSC在早期和晚期传代中均保持强劲的扩增能力及三系分化潜能。
支持直接从人类原代组织中衍生MSC。

产品组分包括

MesenCult™ -ACF Plus基础培养基（500 mL）
MesenCult™-ACF Plus 500X 补充剂（1 mL）
无动物成分细胞贴附基质（1 mL）

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总览

使用MesenCult™-ACF Plus培养试剂盒减少人间充质基质细胞（MSC）培养的变异性并提高可重复性。该试剂盒不含动物源性成分（ACF）和细胞外囊泡（EV），并针对多种来源（包括骨髓和脂肪组织）的MSC的无血清诱导和扩增进行了优化。

MesenCult™-ACF Plus支持高效的MSC扩增，同时保持干净、无EV的培养背景。由于该配方不含外源EVs，您可以直接从条件培养基中分离MSC来源的EVs，而无需切换到专门的EV收集培养基。有关使用此系统生成EV的指导说明，请参阅我们详细的EV生成方案。将该试剂盒与EasySep™人细胞外囊泡正选试剂盒</a>配合使用，以实现高效和标准化的EV回收。

与含血清或去除EV的培养基相比，在MesenCult™-ACF Plus中培养的MSC能够更高效地扩增并保持标志性的MSC特征，包括表面标志物表达、强效增殖以及可通过MesenCult™-ACF软骨生成分化试剂盒、MesenCult™脂肪生成分化试剂盒（人）和MesenCult™成骨分化试剂盒（人）进行评估的三谱系分化潜能。

该试剂盒是更广泛的MesenCult™ MSC细胞培养产品组合的一部分，该产品组合包含用于MSC分化、培养和鉴定的试剂。您还可以使用人iPSC衍生的间充质祖细胞，从经过验证、纯的MSC培养物开始您的实验。

注意：MesenCult™-ACF Plus完全培养基必须添加L-谷氨酰胺。CollPlant是细胞贴附基质中重组人胶原蛋白（rhCollagen）成分的制造商。

实验数据

Figure 1. CFU-F Assay of Human BM-Derived MSCs Expanded in MesenCult™-ACF Plus Medium and Commercial Media.
(A) An average of 45 CFU-Fs per million cells were observed when BM mononuclear cells were seeded in MesenCult™-ACF Plus (n = 4). An average of 47 and 25 CFU-Fs per million cells were observed when cells were seeded in Commercial Medium 1 (n = 3) and Medium 2 (n = 4), respectively. Vertical lines indicate Standard Error of Mean (SEM). Representative image of CFU-F colonies expanded in (B) MesenCult™-ACF Plus Medium (9 days of culture), (C) Commercial Medium 1 (10 days of culture) and (D) Commercial Medium 2 (10 days of culture). Commercial Medium 1 and Medium 2 were supplemented with 2.5% human AB serum to derive MSCs from BM, as per their protocols for derivation. No addition of serum is required when using MesenCult™-ACF Plus Medium.

Figure 2. Human BM-Derived MSCs Cultured in MesenCult™-ACF Plus Medium Expand Faster than MSCs Cultured in Commercial Xeno-Free and Serum-Free Media.
(A) A greater number of BM-derived MSCs were generated per passage using MesenCult™-ACF Plus Medium (n=4) compared to Commercial Medium 1 (n=3) and Commercial Medium 2 (n=2). (B) Rates of BM-derived MSC expansion were compared between MesenCult™-ACF Plus Medium, Commercial Medium 1, and Commercial Medium 2. The time required to double the number of MSCs using MesenCult™ -ACF Plus Medium (n=4) was shorter than when MSCs were cultured in Commercial Medium 1 (n=3) and Commercial Medium 2 (n=4). Vertical lines indicate Standard Error of Mean (SEM).

Figure 3. Human BM-Derived MSCs Expanded in MesenCult™-ACF Plus Medium Display Multi-Lineage Differentiation Potential.
(A) Human BM-derived MSCs expanded in MesenCult™-ACF Plus Medium differentiated into (B) adipocytes (Oil Red O staining; passage 5), (C) chondrocytes (Alcian Blue staining; passage 4) and (D) osteoblasts (Alizarin Red S staining; passage 5).

Figure 4. Flow Cytometric Analysis of MSCs Cultured in MesenCult™-ACF Plus Medium.
BM-derived MSCs were cultured and expanded in MesenCult™-ACF Plus Medium. At passage 8 MSCs were stained for mesenchymal surface markers (CD73, CD90, CD105,), pericyte marker (CD146) and hematopoietic marker (CD45). MSCs expressed high levels of CD73, CD90, CD105 and CD146 and lacked expression of CD45.

产品说明书及文档

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05448

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文献 (11)

Dynamic stimulation promotes functional tissue-like organization of a 3D human lymphoid microenvironment model in vitro D. Barozzi et al. Cell Reports Methods 2025 Jul

Abstract

This work focused on generating a three-dimensional (3D) in vitro dynamic model to study chronic lymphocytic leukemia (CLL) cell dissemination, homing, and mechanisms of therapy resistance. We used a gelatin-based, hard porous biomaterial as a support matrix to develop 3D tissue-like models of the human lymph node and bone marrow, which were matured inside bioreactors under dynamic perfusion of medium. Comparing static and dynamic cultures of these 3D constructs revealed that perfusion promoted a tissue-like internal organization of cells, characterized by the expression of specific functional markers and deposition of an intricate extracellular matrix protein network. Recirculation of CLL cells within the dynamic system led to changes in leukemic cell behavior and in the expression of key markers involved in tumor progression. These findings suggest that the model is well suited for investigating the pathophysiological mechanisms of CLL and potentially other hematological malignancies.

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Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells Cells 2024 Sep

Abstract

Differentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells (ESCs) from early mesoderm to MSCs using serum-containing or xeno-free differentiation medium and observed differences in the cells’ morphology, doubling rate, ability to form colonies, surface marker analysis, and multilineage differentiation potential of the obtained cell lines. We concluded that the xeno-free medium best fits the criteria of MSCs’ morphology, growth kinetics, and surface marker characterization. In contrast, the serum-containing medium gives better potential for further MSC differentiation into osteogenic, chondrogenic, and adipogenic lineages.

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Genome-wide sequencing identified extrachromosomal circular DNA as a transcription factor-binding motif of the senescence genes that govern replicative senescence in human mesenchymal stem cells W. Yang et al. Frontiers in Cellular Neuroscience 2024 Aug

Abstract

Mesenchymal stem cells (MSCs) have long been postulated as an important source cell in regenerative medicine. During subculture expansion, mesenchymal stem cell (MSC) senescence diminishes their multi-differentiation capabilities, leading to a loss of therapeutic potential. Up to date, the extrachromosomal circular DNAs (eccDNAs) have been demonstrated to be involved in senescence but the roles of eccDNAs during MSC. Here we explored eccDNA profiles in human bone marrow MSCs (BM-MSCs). EccDNA and mRNA was purified and sequenced, followed by quantification and functional annotation. Moreover, we mapped our datasets with the downloading enhancer and transcription factor-regulated genes to explore the potential role of eccDNAs. Sequentially, gene annotation analysis revealed that the majority of eccDNA were mapped in the intron regions with limited BM-MSC enhancer overlaps. We discovered that these eccDNA motifs in senescent BMSCs acted as motifs for binding transcription factors (TFs) of senescence-related genes. These findings are highly significant for identifying biomarkers of senescence and therapeutic targets in mesenchymal stem cells (MSCs) for future clinical applications. The potential of eccDNA as a stable therapeutic target for senescence-related disorders warrants further investigation, particularly exploring chemically synthesized eccDNAs as transcription factor regulatory elements to reverse cellular senescence.

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物种	人类
配方	不含动物成分, 无血清

MesenCult™-ACF Plus培养试剂盒

产品号 #05448

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产品号 #05448

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MesenCult™-ACF Plus培养试剂盒

产品号 #05448

产品号 #（选择产品）

产品号 #05448

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

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