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MesenCult™-ACF Plus培养试剂盒

用于人间充质干细胞的无动物成分培养基及贴附基质
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产品号 #05448

用于人间充质干细胞的无动物成分培养基及贴附基质

产品优势

  • 无动物成分配方提升实验可重复性。
  • 相较于含血清培养基，具备更优异的细胞扩增效果。
  • 培养的MSC在早期和晚期传代中均保持强劲的扩增能力及三系分化潜能。
  • 支持直接从人类原代组织中衍生MSC。

产品组分包括

  • MesenCult™ -ACF Plus基础培养基（500 mL）
  • MesenCult™-ACF Plus 500X 补充剂（1 mL）
  • 无动物成分细胞贴附基质 （1 mL）
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. L-谷氨酰胺
    L-谷氨酰胺
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用这款无动物成分(ACF)且不含细胞外囊泡(EV)的培养基，可降低人间充质基质细胞(MSC，亦称间充质干细胞)培养的差异性，提升实验可重复性。MesenCult™-ACF Plus培养试剂盒经过优化，无需血清即可从骨髓或脂肪组织等多种来源衍生人类MSC。

与含血清或EV去除的含血清培养基相比，使用本试剂盒培养的MSC扩增效率更高（增殖速率与累计细胞总量），且功能不受影响。培养细胞表达典型的MSC表面标志物，并保持强劲的扩增速率和三系分化能力。

本试剂盒是完整ACF产品线的一部分——涵盖MSC的衍生、扩增、冻存；以及将人多能干细胞分化为间充质祖细胞——专为高效稳定的MSC培养而优化。

为实现无动物成分的优化的细胞冻存目的，推荐使用MesenCult™-ACF冻存培养基冻存先前在MesenCult™培养基（包括MesenCult™-ACF Plus）中培养的人类MSC。有关相关产品的完整列表，包括可用的分化培养基，请访问 our MSC area of interest page，或通过 techsupport@stemcell.com 联系我们获取。

注意：MesenCult™-ACF Plus完全培养基必须添加L-谷氨酰胺。配制完全培养基所需的培养基与补充剂（不含基质）亦可单独购买，即MesenCult™-ACF Plus培养基。

CollPlant是细胞贴附基质中重组人胶原蛋白(rhCollagen)组分的生产商。

本产品仅限研究使用。如有任何临床或商业应用需求，请联系STEMCELL公司。

分类
专用培养基
 
细胞类型
间充质细胞，PSC衍生，间充质干/祖细胞
 
种属
人
 
应用
细胞培养，扩增，培养
 
品牌
MesenCult
 
研究领域
细胞外囊泡研究，干细胞生物学
 
制剂类别
不含动物成分，无血清
 

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实验数据

Figure 1. CFU-F Assay of Human BM-Derived MSCs Expanded in MesenCult™-ACF Plus Medium and Commercial Media.
(A) An average of 45 CFU-Fs per million cells were observed when BM mononuclear cells were seeded in MesenCult™-ACF Plus (n = 4). An average of 47 and 25 CFU-Fs per million cells were observed when cells were seeded in Commercial Medium 1 (n = 3) and Medium 2 (n = 4), respectively. Vertical lines indicate Standard Error of Mean (SEM). Representative image of CFU-F colonies expanded in (B) MesenCult™-ACF Plus Medium (9 days of culture), (C) Commercial Medium 1 (10 days of culture) and (D) Commercial Medium 2 (10 days of culture). Commercial Medium 1 and Medium 2 were supplemented with 2.5% human AB serum to derive MSCs from BM, as per their protocols for derivation. No addition of serum is required when using MesenCult™-ACF Plus Medium.

Figure 2. Human BM-Derived MSCs Cultured in MesenCult™-ACF Plus Medium Expand Faster than MSCs Cultured in Commercial Xeno-Free and Serum-Free Media.
(A) A greater number of BM-derived MSCs were generated per passage using MesenCult™-ACF Plus Medium (n=4) compared to Commercial Medium 1 (n=3) and Commercial Medium 2 (n=2). (B) Rates of BM-derived MSC expansion were compared between MesenCult™-ACF Plus Medium, Commercial Medium 1, and Commercial Medium 2. The time required to double the number of MSCs using MesenCult™ -ACF Plus Medium (n=4) was shorter than when MSCs were cultured in Commercial Medium 1 (n=3) and Commercial Medium 2 (n=4). Vertical lines indicate Standard Error of Mean (SEM).

Figure 3. Human BM-Derived MSCs Expanded in MesenCult™-ACF Plus Medium Display Multi-Lineage Differentiation Potential.
(A) Human BM-derived MSCs expanded in MesenCult™-ACF Plus Medium differentiated into (B) adipocytes (Oil Red O staining; passage 5), (C) chondrocytes (Alcian Blue staining; passage 4) and (D) osteoblasts (Alizarin Red S staining; passage 5).

Figure 4. Flow Cytometric Analysis of MSCs Cultured in MesenCult™-ACF Plus Medium.
BM-derived MSCs were cultured and expanded in MesenCult™-ACF Plus Medium. At passage 8 MSCs were stained for mesenchymal surface markers (CD73, CD90, CD105,), pericyte marker (CD146) and hematopoietic marker (CD45). MSCs expressed high levels of CD73, CD90, CD105 and CD146 and lacked expression of CD45.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Plus Culture Kit
Catalog #
05448
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MesenCult™-ACF Plus Culture Kit
Catalog #
05448
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MesenCult™-ACF Plus Culture Kit
Catalog #
05448
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MesenCult™-ACF Plus Culture Kit
Catalog #
05448
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (13)

Products for Your Mesenchymal Stromal Cell Research
Brochure
Products for Your Mesenchymal Stromal Cell Research
qPCR Arrays for Cell Characterization
Brochure
qPCR Arrays for Cell Characterization
MesenCult™-ACF Plus
Brochure
MesenCult™-ACF Plus
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
Technical Bulletin
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
SnapShot: Adipocyte Life Cycle
Wallchart
SnapShot: Adipocyte Life Cycle
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Correlating TEER Values with Air-Liquid Interface Culture Morphology
3:02
Video
Correlating TEER Values with Air-Liquid Interface Culture Morphology
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
1:51
Video
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
The Secret In Vivo Life of "Mesenchymal Stem Cells"
58:00
Webinar
The Secret In Vivo Life of "Mesenchymal Stem Cells"
Tools to Accelerate Your MSC and EV Workflows
30:05
Webinar
Tools to Accelerate Your MSC and EV Workflows
The Impact of Ancillary Materials on Your Translational Research
17:18
Webinar
The Impact of Ancillary Materials on Your Translational Research
Mesenchymal Stromal Cells: Markers, Isolation and Culture, Differentiation, and Therapeutic Potential
Mini Review
Mesenchymal Stromal Cells: Markers, Isolation and Culture, Differentiation, and Therapeutic Potentia...

文献 (11)

Dynamic stimulation promotes functional tissue-like organization of a 3D human lymphoid microenvironment model in vitro D. Barozzi et al. Cell Reports Methods 2025 Jul

Abstract

This work focused on generating a three-dimensional (3D) in vitro dynamic model to study chronic lymphocytic leukemia (CLL) cell dissemination, homing, and mechanisms of therapy resistance. We used a gelatin-based, hard porous biomaterial as a support matrix to develop 3D tissue-like models of the human lymph node and bone marrow, which were matured inside bioreactors under dynamic perfusion of medium. Comparing static and dynamic cultures of these 3D constructs revealed that perfusion promoted a tissue-like internal organization of cells, characterized by the expression of specific functional markers and deposition of an intricate extracellular matrix protein network. Recirculation of CLL cells within the dynamic system led to changes in leukemic cell behavior and in the expression of key markers involved in tumor progression. These findings suggest that the model is well suited for investigating the pathophysiological mechanisms of CLL and potentially other hematological malignancies.
View publication
Alternative Ways to Obtain Human Mesenchymal Stem Cells from Embryonic Stem Cells Cells 2024 Sep

Abstract

Differentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells (ESCs) from early mesoderm to MSCs using serum-containing or xeno-free differentiation medium and observed differences in the cells’ morphology, doubling rate, ability to form colonies, surface marker analysis, and multilineage differentiation potential of the obtained cell lines. We concluded that the xeno-free medium best fits the criteria of MSCs’ morphology, growth kinetics, and surface marker characterization. In contrast, the serum-containing medium gives better potential for further MSC differentiation into osteogenic, chondrogenic, and adipogenic lineages.
View publication
Genome-wide sequencing identified extrachromosomal circular DNA as a transcription factor-binding motif of the senescence genes that govern replicative senescence in human mesenchymal stem cells W. Yang et al. Frontiers in Cellular Neuroscience 2024 Aug

Abstract

Mesenchymal stem cells (MSCs) have long been postulated as an important source cell in regenerative medicine. During subculture expansion, mesenchymal stem cell (MSC) senescence diminishes their multi-differentiation capabilities, leading to a loss of therapeutic potential. Up to date, the extrachromosomal circular DNAs (eccDNAs) have been demonstrated to be involved in senescence but the roles of eccDNAs during MSC. Here we explored eccDNA profiles in human bone marrow MSCs (BM-MSCs). EccDNA and mRNA was purified and sequenced, followed by quantification and functional annotation. Moreover, we mapped our datasets with the downloading enhancer and transcription factor-regulated genes to explore the potential role of eccDNAs. Sequentially, gene annotation analysis revealed that the majority of eccDNA were mapped in the intron regions with limited BM-MSC enhancer overlaps. We discovered that these eccDNA motifs in senescent BMSCs acted as motifs for binding transcription factors (TFs) of senescence-related genes. These findings are highly significant for identifying biomarkers of senescence and therapeutic targets in mesenchymal stem cells (MSCs) for future clinical applications. The potential of eccDNA as a stable therapeutic target for senescence-related disorders warrants further investigation, particularly exploring chemically synthesized eccDNAs as transcription factor regulatory elements to reverse cellular senescence.
View publication
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更多信息
物种 人类
配方 不含动物成分, 无血清
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