EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #70008_C
冻存的人原代细胞
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HLA information from single donor CB CD34+ products can be provided upon request for specific lots. Please reach out to your sales representative or contact us directly for more information.
HLA information from single donor CB CD34+ products can be provided upon request for specific lots. Please reach out to your sales representative or contact us directly for more information.
总览
选择即用型、符合伦理的人CD34+干细胞和祖细胞助您轻松开启实验。我们提供个性化的服务、定制产品、灵活的交付周期，并支持提前测试筛选并预留整个批次，以确保您获得所需的细胞。
通过免疫磁珠正选从脐带血中分离细胞，遵循机构审查委员会（IRB）批准的知情同意书和方案进行收集，并冻存于不含动物成分的CryoStor® CS10冻存液（产品号#07930）中。如有需要，可提供其他文档、高分辨率HLA分型（I类和II类等位基因）以及CMV状态。采集过程中添加檬酸盐-磷酸盐-葡萄糖（CPD）作为抗凝剂。选择产品选项后，供体信息（如BMI范围、年龄、种族等）可以在上面的评论框中查询。所有供体均经过HIV-1/2、乙肝和丙肝筛查。
CD34+干细胞和祖细胞可以与许多产品结合使用，包括StemSpan™、MethoCult™、MegaCult™和MyeloCult™，以扩增和分化其他稀有细胞类型。
某些产品仅在特定地区出售。请与您当地的销售代表或产品与科学支持联系techsupport@stemcell.com获取更多信息。
欲了解更多信息，请浏览有关原代细胞的常见问题解答（FAQs）。
包含
• 无血清冻存液
• 10% 二甲基亚砜 (DMSO)
分类
冻存
细胞类型
造血干/祖细胞
种属
人
细胞和组织来源
脐带血
研究领域
药物发现和毒性检测
供体状态
正常
纯度
流式细胞术检测：CD34+ （占 CD45+ 细胞的百分比）≥ 90%
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (9)
文献 (16)
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53.
Blood 2009 SEP
Abstract
The role of miRNAs in regulating megakaryocyte differentiation was examined using bipotent K562 human leukemia cells. miR-34a is strongly up-regulated during phorbol ester-induced megakaryocyte differentiation,but not during hemin-induced erythrocyte differentiation. Enforced expression of miR-34a in K562 cells inhibits cell proliferation,induces cell-cycle arrest in G(1) phase,and promotes megakaryocyte differentiation as measured by CD41 induction. miR-34a expression is also up-regulated during thrombopoietin-induced differentiation of CD34(+) hematopoietic precursors,and its enforced expression in these cells significantly increases the number of megakaryocyte colonies. miR-34a directly regulates expression of MYB,facilitating megakaryocyte differentiation,and of CDK4 and CDK6,to inhibit the G(1)/S transition. However,these miR-34a target genes are down-regulated rapidly after inducing megakaryocyte differentiation before miR-34a is induced. This suggests that miR-34a is not responsible for the initial down-regulation but may contribute to maintaining their suppression later on. Previous studies have implicated miR-34a as a tumor suppressor gene whose transcription is activated by p53. However,in p53-null K562 cells,phorbol esters induce miR-34a expression independently of p53 by activating an alternative phorbol ester-responsive promoter to produce a longer pri-miR-34a transcript.
Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells.
Nature genetics 2009 SEP
Abstract
The reprogramming of somatic cells into induced pluripotent stem (iPS) cells upon overexpression of the transcription factors Oct4,Sox2,Klf4 and cMyc is inefficient. It has been assumed that the somatic differentiation state provides a barrier for efficient reprogramming; however,direct evidence for this notion is lacking. Here,we tested the potential of mouse hematopoietic cells at different stages of differentiation to be reprogrammed into iPS cells. We show that hematopoietic stem and progenitor cells give rise to iPS cells up to 300 times more efficiently than terminally differentiated B and T cells do,yielding reprogramming efficiencies of up to 28%. Our data provide evidence that the differentiation stage of the starting cell has a critical influence on the efficiency of reprogramming into iPS cells. Moreover,we identify hematopoietic progenitors as an attractive cell type for applications of iPS cell technology in research and therapy.
Cited2 is an essential regulator of adult hematopoietic stem cells.
Cell stem cell 2009 DEC
Abstract
The regulatory pathways necessary for the maintenance of adult hematopoietic stem cells (HSCs) remain poorly defined. By using loss-of-function approaches,we report a selective and cell-autonomous requirement for the p300/CBP-binding transcriptional coactivator Cited2 in adult HSC maintenance. Conditional deletion of Cited2 in the adult mouse results in loss of HSCs causing multilineage bone marrow failure and increased lethality. In contrast,conditional ablation of Cited2 after lineage specification in lymphoid and myeloid lineages has no impact on the maintenance of these lineages. Additional deletion of Ink4a/Arf (encoding p16(Ink4a) and p19(Arf)) or Trp53 (encoding p53,a downstream target of p19(Arf)) in a Cited2-deficient background restores HSC functionality and rescues mice from bone marrow failure. Furthermore,we show that the critical role of Cited2 in primitive hematopoietic cells is conserved in humans. Taken together,our studies provide genetic evidence that Cited2 selectively maintains adult HSC functions,at least in part,via Ink4a/Arf and Trp53.
更多信息
| 物种 | 人 |
|---|---|
| Contains | • Serum-free cryopreservation medium • 10% dimethyl sulfoxide (DMSO) |
| 纯度 | ≥ 90% CD34+ (as a percentage of CD45+ cells) by flow cytometry |
| 细胞与组织来源 | 脐带血 |
| 捐献者身份 | 正常 |
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