EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #02691_C
人CD34+造血细胞扩增的无血清培养补充物
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总览
StemSpan™ CD34+扩增添加物（10X）包含重组人细胞因子和其他添加物的组合，用于选择性地促进从人脐带血（CB）或骨髓（BM）样本中分离的CD34+细胞的扩增。StemSpan™ CD34+扩增添加物通常可在7天的液体培养中，促进CD34+人脐带血 (CB) 细胞（总有核细胞）扩增约40倍。一周后，约40%的培养细胞表达CD34，表明接种的CD34+ CB细胞扩增了约10倍。添加UM729等小分子可进一步促进扩增。请查看数据了解更多详细信息。
StemSpan™ CD34+扩增添加物（10X）可与以下任何StemSpan™培养基结合使用：
•StemSpan™ SFEM（产品号 #09600）
•StemSpan™ SFEM II（产品号 #09605）
•StemSpan™-XF（产品号 #100-0073）
•StemSpan™-AOF（产品号 #100-0130）
包含
• 重组人FMS样酪氨酸激酶3配体（Flt3L）
• 重组人干细胞因子（SCF）
• 重组人白介素3（IL-3）
• 重组人白介素6（IL-6）
• 重组人血小板生成素（TPO）
• 其他添加剂
分类
添加剂
细胞类型
造血干/祖细胞
种属
人
应用
细胞培养，扩增
品牌
StemSpan
研究领域
干细胞生物学，移植研究
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (9)
文献 (2)
Isolation and Culture of Non-adherent Cells for Cell Reprogramming. Journal of stem cells & regenerative medicine 2022
Abstract
Coronary heart disease (CHD) is a leading cause of death globally, while its current management is limited to reducing the myocardial infarction area without actually replacing dead cardiomyocytes. Direct cell reprogramming is a method of cellular cardiomyoplasty which aims for myocardial tissue regeneration, and CD34+ cells are one of the potential sources due to their shared embryonic origin with cardiomyocytes. However, the isolation and culture of non-adherent CD34+ cells is crucial to obtain adequate cells for high-efficiency genetic modification. This study aimed to investigate the optimal method for isolation and culture of CD34+ peripheral blood cells using certain culture media. A peripheral blood sample was obtained from a healthy subject and underwent pre-enrichment, isolation, and expansion. The culture was subsequently observed for their viability, adherence, and confluence. Day 0 observation of the culture showed a healthy CD34+ cell with a round cell shape, without any adherent cells present yet. Day 4 of observation showed that CD34+ cells within the blood plasma medium became adherent, indicated by their transformations into spindle or oval morphologies. Meanwhile, CD34+ cells in vitronectin and fibronectin media showed no adherent cells and many of them died. Day 7 observation revealed more adherent CD34+ cells in blood plasma medium, and which had 75% of confluence. In conclusion, the CD34+ cells that were isolated using a combination of density and magnetic methods may be viable and adequately adhere in culture using blood plasma medium, but not in cultures using fibronectin and vitronectin.
Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell 2018 JAN
Abstract
Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previously classified as interferon (IFN) stimulated genes (ISGs) but that expression is intrinsic, as stem cells are refractory to interferon. This intrinsic ISG expression varies in a cell-type-specific manner, and many ISGs decrease upon differentiation, at which time cells become IFN responsive, allowing induction of a broad spectrum of ISGs by IFN signaling. Importantly, we show that intrinsically expressed ISGs protect stem cells against viral infection. We demonstrate the in vivo importance of intrinsic ISG expression for protecting stem cells and their differentiation potential during viral infection. These findings have intriguing implications for understanding stem cell biology and the evolution of pathogen resistance.
更多信息
| 物种 | 人类 |
|---|---|
| Contains | • Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L) • Recombinant human stem cell factor (SCF) • Recombinant human interleukin 3 (IL-3) • Recombinant human interleukin 6 (IL-6) • Recombinant human thrombopoietin (TPO) • Other additives |
| 配方 | 无血清 |
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StemSpan™ SFEM用于培养和扩增造血细胞的无血清培养基
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StemSpan™ SFEM II用于培养和扩增造血细胞的无血清培养基
