EasySep™小鼠TIL（CD45）正选试剂盒
MethoCult™ 甲基纤维素基培养基
在集落形成单位(CFU)检测中识别与定量造血祖细胞
MethoCult™ 是一系列半固态甲基纤维素培养基，专为促进造血祖细胞在培养中的最佳生长和分化而配制。MethoCult™ 是体外检测和定量造血祖细胞的"金标准"，适用于 集落形成单位(CFU) 或细胞（CFC）测定，也称为甲基纤维素检测。提供多种 MethoCult™ 培养基配方，用于人和小鼠组织来源的造血细胞CFU检测。特定配方还可用于人多能干细胞(hPSCs)或其他物种(如大鼠、非人灵长类和犬类)来源的造血细胞。
为何使用 MethoCult™ ？
- 采用严格筛选的组分制备。
- 经过严格的性能测试，确保卓越的批次间可重复性。
- 提供即用型配方，可鉴定和计数总CFU、红系(CFU-E和BFU-E)、粒细胞/巨噬细胞(CFU-GM、CFU-G和CFU-M)以及多谱系(CFU-GEMM)祖细胞。
- 也提供允许研究人员自行添加所需组分的配方。可根据需求提供定制配方和规格。
含血清
规格：
- 80-100 mL 瓶装
- 3 mL × 24 管装
含血清:
是
含细胞因子:
可选择已添加或未添加重组细胞因子
组织
- 脐带血
- 骨髓
- 外周血
- 白细胞单采术样本
- 纯化或培养的 HSPCs
应用
- 快速 - 脐带血库快速CFU检测方案
- 经典 - 历史方案原始配方
- 优化 - 改进的髓系集落亚型区分度
- 富集 - 适用于纯化/培养的HSPCs及LTC-IC检测
- 支持髓系 (CFU-G/M/GM)、混合系 (CFU-GEMM) 和/或红系 (CFU-E/BFU-E) 集落生长
特点:
- 配方可含或不含 EPO
- 兼容 STEMvision™ 仪器，用于CFU检测的自动化标准化计数
- 有能力验证 （Proficiency Testing） 方案
无血清
成分
80-100 mL 瓶装
含血清:
否
含细胞因子:
可选择已添加或未添加重组细胞因子
组织:
- 脐带血
- 骨髓
- 外周血
- 白细胞单采术样本
- 纯化或培养的 HSPCs
- hPSC 来源细胞
应用:
- 适用于需要明确成分培养基的检测
- 支持髓系 (CFU-G/M/GM) 集落生长，无论是否存在混合系 (CFU-GEMM) 集落
特点:
- 完全无血清且成分明确
- 配方可含或不含 EPO 或其他细胞因子
条件培养基
成分
- 100 mL 瓶装
含血清:
是
含细胞因子:
可含或不含重组EPO的条件培养基
组织:
- 脐带血
- 骨髓
- 外周血
- 白细胞单采术样本
- 纯化或培养的HSPCs
应用:
- 使用琼脂-白细胞条件培养基 (Agar-LCM) 作为集落刺激因子来源的检测
- 支持髓系(CFU-G/M/GM)、混合系(CFU-GEMM)和/或红系(CFU-E/BFU-E)集落生长
特点:
- 含血清及补充成分
- 配方可含或不含EPO
基础甲基纤维素
成分
40 mL 瓶装
含血清:
否
包含细胞因子:
否
组织:
- 脐带血
- 骨髓
- 外周血
- 白细胞单采术样本
- 纯化或培养的HSPCs
应用:
不含血清、不含细胞因子的配方，适用于测试血清或细胞因子
特点:
- 可灵活添加单一细胞因子和补充剂
- 基础甲基纤维素2.6%母液，溶于Iscove改良杜氏培养基
收起详情
不确定哪种 MethoCult™ 配方适合您？
在CFU检测中获得强健的集落生长，最适MethoCult™配方取决于您的起始细胞来源。使用我们的交互式产品查找工具，确定最适合您实验的配方，并为您的CFU检测生成完整的材料清单。
含血清
成分:
- 90 mL 或 100 mL 瓶装
- 3 ml × 24 管装
含血清:
是
包含细胞因子:
可选择已添加或未添加重组细胞因子
组织:
- 骨髓
- 外周血
- 脾脏
- 胎肝
应用:
支持髓系 (CFU-G/M/GM)、混合系 (CFU-GEMM) 和/或红系 (CFU-E/BFU-E) 祖细胞的集落生长
特点:
- 配方可含或不含 EPO
- 选择与 STEMvision™ 仪器兼容的配方，实现CFU检测的自动化标准化计数
无血清
成分:
100 mL 瓶装
含血清:
否
含细胞因子:
已添加重组细胞因子
组织:
- 骨髓
- 外周血
- 脾脏
- 肝脏
应用:
支持红系 (BFU-E) 祖细胞的集落生长
特点:
- 含 EPO 配方
- 兼容 STEMvision™ 仪器，用于 CFU 检测的自动化标准化计数
基础甲基纤维素
成分:
80 mL 或 40 mL 瓶装
含血清:
否
含细胞因子:
否
组织:
- 骨髓
- 外周血
- 脾脏
- 胎肝
应用:
不含血清、不含细胞因子的配方，适用于测试血清或细胞因子
特点:
- 可灵活添加单一细胞因子和补充剂
- 基础甲基纤维素2.6%母液，溶于 Iscove 改良杜氏培养基
其他物种
成分:
100 mL瓶装
含血清:
是
含细胞因子:
已添加重组细胞因子
组织:
- 骨髓
- 非人灵长类动物骨髓、外周血及纯化 HSPCs
应用:
- 大鼠髓系 (CFU-GM/G/M) 或红系 (CFU-E/BFU-E) 祖细胞定量
- 非人灵长类动物髓系(CFU-GM)、混合系 (CFU-GEMM) 和/或红系 (CFU-E/BFU-E) 祖细胞定量
特点:
- 大鼠GF R3774配方专为检测大鼠粒细胞/巨噬细胞祖细胞优化
- 小鼠SF M3436配方适用于大鼠红系祖细胞的生长与计数
- 多款人用经典、优化及富集配方适用于非人灵长类动物样本
收起详情
实验数据
图1所示。人类脐带血菌落镀MethoCult™Optimum H4034，影像来源：STEMvision™工具
图2。小鼠骨髓菌落镀于MethoCult™GF M3434，影像来源：STEMvision™工具
品牌历史
STEMCELL 与 MethoCult™ 均源自不列颠哥伦比亚癌症局Terry Fox 血液学/肿瘤学研究实验室的培养基制备服务。如今二十多年过去，MethoCult™已成为造血祖细胞集落形成实验中发表文献最多的甲基纤维素培养基。CFU 检测资源
探索我们的资源，获取关于建立、优化和标准化CFU检测的信息与支持。
Key Applications and Related Publications
Studying Effects of Intrinsic and Extrinsic Regulators on Normal Hematopoietic Progenitor Cell Proliferation and Differentiation
Shin J et al. (2014) High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias. J Exp Med 211(2): 217-31
Zimdahl B et al. (2014) Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nat Genet 46(3):245-52
Baker S et al. (2014) B-myb is an essential regulator of hematopoietic stem cell and myeloid progenitor cell development. Proc Natl Acad Sci 111(8):3122-7
Zimdahl B et al. (2014) Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nat Genet 46(3):245-52
Baker S et al. (2014) B-myb is an essential regulator of hematopoietic stem cell and myeloid progenitor cell development. Proc Natl Acad Sci 111(8):3122-7
Studying Effects of Intrinsic and Extrinsic Regulators on Malignant Hematopoietic Progenitor Cell Proliferation and Differentiation
Li Q et al. (2013) Oncogenic Nras has bimodal effects on stem cells that sustainably increase competitiveness. Nature504(7478):143-7
Zhu X et al. (2014) Identification of functional cooperative mutations of SETD2 in human acute leukemia. Nat Genet46(3):287-93
Xu J et al. (2014) DNMT3A Arg882 mutation drives chronic myelomonocytic leukemia through disturbing gene expression/DNA methylation in hematopoietic cells. Proc Natl Acad Sci111(7):2620-5
Montes R et al. (2014) Ligand-independent FLT3 activationdoes not cooperate with MLL-AF4 to immortalize/transform cord blood CD34+ cells.Leukemia28(3):666-74
Zhu X et al. (2014) Identification of functional cooperative mutations of SETD2 in human acute leukemia. Nat Genet46(3):287-93
Xu J et al. (2014) DNMT3A Arg882 mutation drives chronic myelomonocytic leukemia through disturbing gene expression/DNA methylation in hematopoietic cells. Proc Natl Acad Sci111(7):2620-5
Montes R et al. (2014) Ligand-independent FLT3 activationdoes not cooperate with MLL-AF4 to immortalize/transform cord blood CD34+ cells.Leukemia28(3):666-74
Evaluating Hematopoietic Cell Samples for Stem Cell Transplantation
Yoo K et al. (2007) The impact of post-thaw colony-forming units-granulocyte/macrophage on engraftment following unrelated cord blood transplantation in pediatric recipients. Bone Marrow Transplant 39(9):515-21
Prasad V et al. (2008) Unrelated donor umbilical cord blood transplantation for inherited metabolic disorders in 159 pediatric patients from a single center: influence of cellular composition of the graft on transplantation outcomes. Blood 112(7):2979-89
Page et al. (2011) Total colony-forming units are a strong, independent predictor of neutrophil and platelet engraftment after unrelated umbilical cord blood transplantation: a single-center analysis of 435 cord blood transplants. Biol Blood Marrow Tr 17(9):1362-74
Prasad V et al. (2008) Unrelated donor umbilical cord blood transplantation for inherited metabolic disorders in 159 pediatric patients from a single center: influence of cellular composition of the graft on transplantation outcomes. Blood 112(7):2979-89
Page et al. (2011) Total colony-forming units are a strong, independent predictor of neutrophil and platelet engraftment after unrelated umbilical cord blood transplantation: a single-center analysis of 435 cord blood transplants. Biol Blood Marrow Tr 17(9):1362-74
Optimizing and Evaluating Gene Transfer Protocols
Hanawa H et al. (2014) Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus–based lentiviral vector system. Blood 103(11):4062-9
Aiuti A et al. (2013) Lentiviral hematopoietic stem cell gene therapy in patients with Wiskott-Aldrich syndrome. Science 341(6148):1233151
Biffi A et al. (2013) Lentiviral hematopoietic stem cell gene therapy benefits metachromatic leukodystrophy. Science 341(6148):1233158
Aiuti A et al. (2013) Lentiviral hematopoietic stem cell gene therapy in patients with Wiskott-Aldrich syndrome. Science 341(6148):1233151
Biffi A et al. (2013) Lentiviral hematopoietic stem cell gene therapy benefits metachromatic leukodystrophy. Science 341(6148):1233158
Quantifying Human and Mouse Primitive Hematopoietic Progenitor Cells Following Long-Term Culture-Initiating Cell (LTC-IC) Assays
Hogge D et al. (1996) Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor. Blood 88(10):3765-73
Lemieux M et al. (1995) Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures. Blood 86(4):1339-47
Kirouac D et al. (2010) Dynamic interaction networks in a hierarchically organized tissue. Mol Syst Biol 6:417
Lemieux M et al. (1995) Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures. Blood 86(4):1339-47
Kirouac D et al. (2010) Dynamic interaction networks in a hierarchically organized tissue. Mol Syst Biol 6:417
Quality Control of Cryopreservation, Cell Processing and Ex Vivo Manipulation Procedures
Alonso J et al. (2001) A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences. Cytotherapy 3(6):429-33
Broxmeyer H et al. (2003) High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years.Proc Natl Acad Sci 100(2):645-50
Koliakos G et al. (2007) A novel high-yield volume-reduction method for the cryopreservation of UC blood units. Cytotherapy 9(7):654-9
Broxmeyer H et al. (2003) High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years.Proc Natl Acad Sci 100(2):645-50
Koliakos G et al. (2007) A novel high-yield volume-reduction method for the cryopreservation of UC blood units. Cytotherapy 9(7):654-9
Testing in Vitro Sensitivity of Hematopoietic Progenitor Cells to Candidate Therapeutics for Drug Development
Pessina A et al. (2001) Prevalidation of a model for predicting acute neutropenia by colony forming unit granulocyte/macrophage (CFU-GM) assay. Toxicol In Vitro 15(6):729-40
Pessina A et al. (2003) Application of the CFU-GM assay to predict acute drug-induced neutropenia: an international blind trial to validate a prediction model for the maximum tolerated dose (MTD) of myelosuppressive xenobiotics. Toxicol Sci 75(2):355-67
Pessina A et al. (2003) Application of the CFU-GM assay to predict acute drug-induced neutropenia: an international blind trial to validate a prediction model for the maximum tolerated dose (MTD) of myelosuppressive xenobiotics. Toxicol Sci 75(2):355-67
Evaluating Hematopoietic Differentiation of Induced Pluripotent Stem Cells
Vodyanik M et al. (2006) Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood 108(6):2095-105
Amabile G et al. (2013) In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells. Blood 121(8):1255-64
Amabile G et al. (2013) In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells. Blood 121(8):1255-64
