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hPSC基因检测试剂盒

用于检测人胚胎干细胞（hESCs）和诱导多能干细胞（iPSCs）中大多数核型异常的 qPCR 分析试剂盒。

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产品号 #07550_C

qPCR 分析试剂盒，用于检测人胚胎干细胞（ES）和诱导多能干细胞（iPS）中多数已报道的核型异常。

产品组分包括

  • qPCR Master Mix (2X), 3 mL
  • ROX参比染料, 0.2 mL
  • Chr 1q 基因检测试剂, 60次反应量(Rxn)
  • Chr 4p 基因检测试剂, 60次反应量(Rxn)
  • Chr 8q 基因检测试剂, 60次反应量(Rxn)
  • Chr 10p 基因检测试剂, 60次反应量(Rxn)
  • Chr 12p 基因检测试剂, 60次反应量(Rxn)
  • Chr 17q 基因检测试剂, 60次反应量(Rxn)
  • Chr 18q 基因检测试剂, 60次反应量(Rxn)
  • Chr 20q 基因检测试剂, 60次反应量(Rxn)
  • Chr Xp 基因检测试剂, 60次反应量(Rxn)
  • 基因组DNA对照, 15 µL
  • TE缓冲液, 1 mL
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. Axygen® 定量实时 PCR（qPCR）密封膜
    Axygen® 定量实时 PCR（qPCR）密封膜
  2. 基因组 DNA 纯化试剂盒
    基因组 DNA 纯化试剂盒
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

快速且经济地检测人多能干细胞（hPSCs）中最常见的8种核型异常。

本qPCR试剂盒可对多条hPSC细胞系进行遗传筛查，提供足够的材料用于最多18个样本和2个对照（三重复）分析，包括对照DNA。该试剂盒采用双淬灭探针，使用5-羧基荧光素（5-FAM）染料，性能优于单淬灭探针。内容包括qPCR主混合液、ROX参考染料、缓冲液，以及用于检测8个常见突变区域关键最小区域的引物/探针混合物和一个基因组对照引物/探针混合物。另附带在目标区域具有正常拷贝数的基因组DNA对照。

结果可输入在线Genetic Analysis App进行统计分析、数据解释与可视化。

 

细胞类型
多能干细胞
 
种属
人
 
应用
基因组编辑
 
研究领域
疾病建模，药物发现和毒理检测，干细胞生物学
 

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实验数据

Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy

Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation

Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication

Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).

Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism

Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 6
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
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English
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Safety Data Sheet 8
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 9
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
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Language
English
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Safety Data Sheet 10
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 11
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
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Language
English
Document Type
Safety Data Sheet 12
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 13
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

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Workflow Stages

相关材料与文献

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Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
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