(A) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 77.2 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.6% and 77.6%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 86.1 ± 7.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.2% and 91.6%, respectively.
C) Starting with naïve C57BL/6 mouse bone marrow, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 62.0 ± 13.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.3% and 71.1%, respectively. (D) Starting with immunized BALB/c mouse bone marrow, the plasma cell (CD138+CD267 (TACI)+) content of the isolated fraction is typically 85.5 ± 9.8% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 0.6% and 89.0%, respectively.
(E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 3.2 ± 4.0% and 56.1 ± 37.2% (mean ± SD), respectively. (F) Isolated CD138+ cells or total bone marrow cells from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total bone marrow cells and CD138+ cells were 0% and 31.9 ± 46.0% (mean ± SD), respectively.
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