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EasySep™人总粒细胞分选试剂盒

对来源于新鲜外周血白细胞进行免疫磁珠负选分离，获得未标记的泛粒细胞（中性粒、嗜酸性粒、嗜碱性粒）

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产品号 #（选择产品）

产品号 #19259_C

免疫磁珠负选试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达99%
  • 获得不带标记的活细胞

产品组分包括

  • EasySep™人总粒细胞分选试剂盒（产品号 #19259）
    • EasySep™人总粒细胞分选抗体混合物，1 mL
    • EasySep™ D2 Magnetic Particles磁珠，2 x 1 mL
  • RoboSep™人总粒细胞分选试剂盒（产品号 #19259RF）
    • EasySep™人总粒细胞分选抗体混合物，1 mL
    • EasySep™ D2 Magnetic Particles磁珠，2 x 1 mL
    • RoboSep™ 缓冲液（产品号 #20104）x 2
    • RoboSep™过滤吸头（产品号 #20125）x 2
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用 EasySep™ 人全粒细胞分离试剂盒，可通过免疫磁性负选法从新鲜人外周血白细胞样本中轻松高效地分离高纯度的泛粒细胞（包括中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞）。EasySep™ 技术在已发表的研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁分选系统的简便性。

在此 EasySep™ 负选步骤中，非目的细胞被抗体复合物和磁性颗粒标记。经 EasySep™ 磁极分离后，带有磁标记的细胞被去除，未标记的目的粒细胞通过倾倒方式收集。磁性细胞分离完成后，目的粒细胞可直接用于流式细胞术、细胞培养或 DNA/RNA 提取等下游应用。、

了解更多关于 EasySep™ 免疫磁性技术的工作原理，或了解如何使用 RoboSep™ 实现全自动化免疫磁性细胞分离。探索更多产品，包括培养基、补充物、抗体等，以优化您的实验流程。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• Easy 50 EasySep™磁极（产品号 #18002）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群
 
种属
人
 
样本来源
PMNC、全血
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Human Pan-Granulocyte Isolation Profile

Figure 1. Typical EasySep™ Human Pan-Granulocyte Isolation Profile

Starting with whole peripheral blood, the total granulocyte content of the isolated fraction typically ranges from 97 - 99%. As shown in the examples above, neutrophils are typically CD66b+CD16+. Eosinophils are typically CD66b+CD16- and basophils are CD66b- and can be further defined as CD203c+CD123+ and IgE+ (not shown).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Pan-Granulocyte Isolation Kit
Catalog #
19259
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (6)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
2:10
Video
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (1)

Lipoxins modulate neutrophil oxidative burst, integrin expression and lymphatic transmigration differentially in human health and atherosclerosis. J. D. Kraft et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 mar

Abstract

Dysregulated chronic inflammation plays a crucial role in the pathophysiology of atherosclerosis and may be a result of impaired resolution. Thus, restoring levels of specialized pro-resolving mediators (SPMs) to promote the resolution of inflammation has been proposed as a therapeutic strategy for patients with atherosclerosis, in addition to standard clinical care. Herein, we evaluated the effects of the SPM lipids, lipoxin A4 (LXA4 ) and lipoxin B4 (LXB4 ), on neutrophils isolated from patients with atherosclerosis compared with healthy controls. Patients displayed altered endogenous SPM production, and we demonstrated that lipoxin treatment in whole blood from atherosclerosis patients attenuates neutrophil oxidative burst, a key contributor to atherosclerotic development. We found the opposite effect in neutrophils from healthy controls, indicating a potential mechanism whereby lipoxins aid the endogenous neutrophil function in health but reduce its excessive activation in disease. We also demonstrated that lipoxins attenuated upregulation of the high-affinity conformation of the CD11b/CD18 integrin, which plays a central role in clot activation and atherosclerosis. Finally, LXB4 enhanced lymphatic transmigration of human neutrophils isolated from patients with atherosclerosis. This finding is noteworthy, as impaired lymphatic function is now recognized as an important contributor to atherosclerosis. Although both lipoxins modulated neutrophil function, LXB4 displayed more potent effects than LXA4 in humans. This study highlights the therapeutic potential of lipoxins in atherosclerotic disease and demonstrates that the effect of these SPMs may be specifically tailored to the need of the individual.
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更多信息

更多信息
物种 人类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • RoboSep™-S (Catalog #21000)
样本来源 PMNC, 全血
Selection Method Negative
标记抗体
  1. 抗人CD123（IL-3Rα）抗体，clone 6H6
    抗人CD123（IL-3Rα）抗体，clone 6H6

    抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠Monoclonal IgG1抗体

  2. 抗人CD16抗体，clone 3G8
    抗人CD16抗体，clone 3G8

    小鼠抗人、恒河猴、食蟹猴CD16 Monoclonal IgG1抗体

  3. 抗人CD45抗体，clone HI30
    抗人CD45抗体，clone HI30

    小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45

  4. 抗人CD66b抗体，clone G10F5
    抗人CD66b抗体，clone G10F5

    小鼠Monoclonal IgM抗体，抗人、黑猩猩CD66b

  5. 抗人CD11b抗体，clone ICRF44
    抗人CD11b抗体，clone ICRF44

    抗人、恒河猴、食蟹猴CD11b的小鼠Monoclonal IgG1抗体

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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