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EasySep™ Direct人Pan-粒细胞分选试剂盒

直接从全血中分离人粒细胞（中性粒、嗜碱性粒、嗜酸性粒）的免疫磁珠分离

率先评论此产品

产品号 #（选择产品）

产品号 #19659_C

全血免疫磁珠负选试剂盒

产品优势

> 99.9%的红细胞去除率，无需密度梯度离心、沉降或裂解
分选获得的细胞纯度高达99%
易于操作、快速，且无需分离柱
分选得到的细胞未被标记

产品组分包括

EasySep™ Direct人Pan-粒细胞分选试剂盒（产品号#19659）

EasySep™ Direct人Pan-粒细胞分选抗体混合物，2 x 2.5 mL
EasySep™ Direct RapidSpheres™磁珠，4 x 2.5 mL

立即询价

总览

实验数据

产品说明书及文档

应用领域

总览

使用 EasySep™ 直接人全粒细胞分离试剂盒，可通过免疫磁性负选法直接从人全血样本中轻松高效地分离高纯度的全粒细胞（中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞），无需密度梯度离心、沉降或红细胞裂解步骤。EasySep™ 技术在已发表研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。

在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物与名为 EasySep™ Direct RapidSpheres™ 的磁性颗粒进行标记。以下细胞将被去除：T 细胞、B 细胞、单核细胞、NK 细胞、红系细胞和血小板。经 EasySep™ 磁体分离后，带有磁性标记的不需要细胞被去除，未标记的目标粒细胞通过倾倒或移液方式转移到新试管中。磁性分离完成后，目标粒细胞可直接用于流式细胞术、功能分析、培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括细胞表征、低温保存及其他相关产品。

磁极兼容性
• EasySep™磁极（产品号 #18000），或
• “The Big Easy” EasySep™磁极（产品号 #18001），或
• Easy 50 EasySep™磁极（产品号 #18002），或
• EasyEights™ EasySep™磁极（产品号 #18103）

分类
细胞分选试剂盒

细胞类型
粒细胞及其亚群

种属
人

样本来源
全血

分选方法
负选

应用
细胞分选

品牌
EasySep

研究领域
免疫

查看更多显示更少

实验数据

Isolated granulocytes obtained using EasySep™ Direct Human Pan-Granulocyte Isolation Kit

Figure 1. Typical EasySep™ Direct Human Pan-Granulocyte Isolation Profile

Starting with human whole blood from normal healthy donors, the typical pan-granulocyte (neutrophil [CD66b+CD16+], eosinophil [CD66b+CD16-] and basophil [CD66b-CD123+]) content of the non-lysed final isolated fraction is 98.4 ± 1.5% (gated on CD45). In the above example, the pan-granulocyte content of the lysed whole blood start sample and the non-lysed final isolated fraction is 61.8% and 99.6% (gated on CD45), respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

EasySep™ Direct Human Pan-Granulocyte Isolation Kit

Catalog #

19659

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Direct Human Pan-Granulocyte Isolation Kit

Catalog #

19659

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Direct Human Pan-Granulocyte Isolation Kit

Catalog #

19659

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for Granulocyte Research

Cell Sourcing

Cell Isolation

Cell Characterization

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Nuclear positioning facilitates amoeboid migration along the path of least resistance. J. Renkawitz et al. Nature 2019 apr

Abstract

During metazoan development, immune surveillance and cancer dissemination, cells migrate in complex three-dimensional microenvironments1-3. These spaces are crowded by cells and extracellular matrix, generating mazes with differently sized gaps that are typically smaller than the diameter of the migrating cell4,5. Most mesenchymal and epithelial cells and some-but not all-cancer cells actively generate their migratory path using pericellular tissue proteolysis6. By contrast, amoeboid cells such as leukocytes use non-destructive strategies of locomotion7, raising the question how these extremely fast cells navigate through dense tissues. Here we reveal that leukocytes sample their immediate vicinity for large pore sizes, and are thereby able to choose the path of least resistance. This allows them to circumnavigate local obstacles while effectively following global directional cues such as chemotactic gradients. Pore-size discrimination is facilitated by frontward positioning of the nucleus, which enables the cells to use their bulkiest compartment as a mechanical gauge. Once the nucleus and the closely associated microtubule organizing centre pass the largest pore, cytoplasmic protrusions still lingering in smaller pores are retracted. These retractions are coordinated by dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning in front of the microtubule organizing centre is a typical feature of amoeboid migration, our findings link the fundamental organization of cellular polarity to the strategy of locomotion.

View publication

High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry. B. Fregin et al. Nature communications 2019

Abstract

In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.

View publication

View All Publications

物种	人类
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000), or • “The Big Easy” EasySep™ Magnet (Catalog #18001), or • Easy 50 EasySep™ Magnet (Catalog #18002), or • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源	全血
Selection Method	Negative

EasySep™ Direct人Pan-粒细胞分选试剂盒

产品号 #19659

产品号 #（选择产品）

产品号 #19659_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

EasySep™ Direct人Pan-粒细胞分选试剂盒

产品号 #19659

产品号 #（选择产品）

产品号 #19659_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (8)

常见问题

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

文献 (2)

Abstract

Abstract

Request Pricing

更多信息

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