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EasySep™人嗜碱性粒细胞分选试剂盒

对来源于 HetaSep™ 处理过的人外周血样本的未标记的嗜碱性粒细胞进行免疫磁珠负选分离

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产品号 #（选择产品）

产品号 #17969_C

13分钟免疫磁珠负选细胞分选试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达98%
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™人嗜碱性粒细胞分选试剂盒（产品号 #17969）
    • EasySep™人嗜碱性粒细胞分选抗体混合物l, 1 mL
    • EasySep™ Dextran RapidSpheres™      磁珠, 2 x 1 mL
  • RoboSep™ 人嗜碱性粒细胞分选试剂盒（含过滤吸头）（产品号#17969RF）
    • EasySep™人嗜碱性粒细胞分选抗体混合物l, 1 mL
    • EasySep™ Dextran RapidSpheres™ 磁珠, 2 x 1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. HetaSep™
    HetaSep™
  2. EasySep™磁极
    EasySep™磁极
  3. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用 EasySep™ 人嗜碱性粒细胞分选试剂盒，通过免疫磁珠负选，可轻松高效地从经 HetaSep™ 处理的人外周血样本中分离高纯度的人嗜碱性粒细胞。EasySep™ 技术结合单克隆抗体的特异性与无柱磁系统的简便性，已在发表的研究中广泛应用超过 20 年。

在此 EasySep™ 负选过程中，不需要的细胞通过抗体复合物和磁珠标记。以下细胞将被去除：中性粒细胞、嗜酸性粒细胞、T 细胞、B 细胞、单核细胞、NK 细胞、树突状细胞、红细胞和祖细胞。使用 EasySep™ 磁极进行无柱分选，磁珠标记的细胞被去除，只需将未标记的嗜碱性粒细胞倒入或移取至新的管中即可。仅需 13 分钟即可完成磁珠分选过程，分离后的嗜碱性粒细胞可直接用于下游应用，例如流式细胞术、细胞培或DNA/RNA 提取。

该分选试剂盒兼容使用 HetaSep™（产品号 #07906）进行红细胞去除后的细胞样本。

本产品可替代 EasySep™ 人嗜碱性粒细胞富集试剂盒（产品号 #19069），实现更快速的细胞分离。了解更多关于 EasySep™ 免疫磁性技术的工作原理，或了解如何使用 RoboSep™ 实现全自动化的免疫磁性细胞分离。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群
 
种属
人
 
样本来源
PMNC、全血
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
2:10
Video
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

文献 (1)

miR-3934 regulates the apoptosis and secretion of inflammatory cytokines of basophils via targeting RAGE in asthma. L. Dou et al. Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 2022 aug

Abstract

BACKGROUND Several miRNAs are now known to have clear connections to the pathogenesis of asthma. The present study focused on the potential role of miR-3934 during asthma development. METHODS miR-3934 was detected as a down-regulated miRNA in basophils by sequencing analysis. Next, the expression levels of miR-3934 in peripheral blood mononuclear cells of 50 asthma patients and 50 healthy volunteers were examined by RT-qPCR methods. The basophils were then treated with AGEs and transfected with miR-3934 mimics. The apoptosis levels were examined by flow cytometry assay; and the expression levels of cytokines were detected using the ELISA kits. Finally, the Western blot was performed to examined the expression of key molecules in the TGF-$\beta$/Smad signaling pathway. RESULTS miR-3934 was down-regulated in the basophils of asthmatic patients. The expression of the pro-inflammatory cytokines IL-6, IL-8 and IL-33 was enhanced in basophils from asthmatic patients, and this effect was partially reversed by transfection of miR-3934 mimics. Furthermore, receiver operating characteristics analysis showed that miR-3934 levels can be used to distinguish asthma patients from healthy individuals. miR-3934 partially inhibited advanced glycation end products-induced increases in basophil apoptosis by suppressing expression of RAGE. CONCLUSION Our results indicate that miR-3934 acts to mitigate the pathogenesis of asthma by targeting RAGE and suppressing TGF-$\beta$/Smad signaling.
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更多信息

更多信息
物种 人类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 PMNC, 全血
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，clone HI30
    抗人CD45抗体，clone HI30

    小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD123（IL-3Rα）抗体，clone 6H6
    抗人CD123（IL-3Rα）抗体，clone 6H6

    抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠Monoclonal IgG1抗体

质量保证:

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