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EasySep™人APC正选试剂盒II

对来源于外周血单个核细胞或其他单细胞悬液中标记 APC 缀合抗体的人细胞进行免疫磁珠正选分离

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产品号 #（选择产品）

产品号 #17661_C

免疫磁珠细胞正选试剂盒

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™人APC正选试剂盒II（产品号 #17661）
    • EasySep™ APC分选抗体混合物，1 mL
    • 抗人CD32 FcR阻断剂，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
  • RoboSep™ 人APC正选试剂盒II（产品号 #17661RF）
    • EasySep™ APC分选抗体混合物，1 mL
    • 抗人CD32 FcR阻断剂，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）
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  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

使用EasySep™人APC正选试剂盒II，通过免疫磁性正选法可从新鲜、冻存的人外周血单个核细胞（PBMCs）或其他单细胞悬液中分离经藻蓝蛋白（APC）偶联抗体标记的高纯度人细胞。EasySep™技术已在超过20年的公开研究中得到广泛应用，结合单克隆抗体特异性与无柱磁系统简便性。

在此正选步骤中，目标细胞与能够识别APC的和磁珠的抗体复合物结合。本试剂盒还含有抗人Fc受体阻断剂，以防止非特异性结合。标记的细胞通过EasySep™磁极分离，未标记细胞被去除，目标细胞保留在试管中。磁珠分选后，APC+细胞可直接用于流式细胞术、细胞培养或基于细胞的检测。

了解更多EasySep™免疫磁性技术或RoboSep™自动化分离系统。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其他物种，血浆，T 细胞
 
种属
人
 
样本来源
白膜层，脐带血，白细胞单采术样本，其他物种，PBMC
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Starting with human PBMCs, the purities of the start and final isolated fractions in the above example are 23.3% and 99.0%, respectively, using an APC-conjugated anti-human CD19 antibody and EasySep™ Human APC Positive Selection Kit II.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human APC Positive Selection Kit II
Catalog #
17661
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human APC Positive Selection Kit II
Catalog #
17661RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human APC Positive Selection Kit II
Catalog #
17661
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human APC Positive Selection Kit II
Catalog #
17661
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human APC Positive Selection Kit II
Catalog #
17661
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human APC Positive Selection Kit II
Catalog #
17661RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human APC Positive Selection Kit II
Catalog #
17661RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human APC Positive Selection Kit II
Catalog #
17661RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human APC Positive Selection Kit II
Catalog #
17661RF
Lot #
All
Language
English

相关材料与文献

技术资料 (7)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...

文献 (1)

Reduced immunosuppressive properties of axitinib in comparison with other tyrosine kinase inhibitors F. Stehle et al. The Journal of Biological Chemistry 2013

Abstract

The multikinase inhibitors sunitinib, sorafenib, and axitinib have an impact not only on tumor growth and angiogenesis, but also on the activity and function of immune effector cells. In this study, a comparative analysis of the growth inhibitory properties and apoptosis induction potentials of tyrosine kinase inhibitors on T cells was performed. Tyrosine kinase inhibitor treatment resulted in a dramatic decrease in T cell proliferation along with distinct impacts on the cell cycle progression. This was at least partially associated with an enhanced induction of apoptosis although triggered by distinct apoptotic mechanisms. In contrast to sunitinib and sorafenib, axitinib did not affect the mitochondrial membrane potential but resulted in an induction or stabilization of the induced myeloid leukemia cell differentiation protein (Mcl-1), leading to an irreversible arrest in the G2/M cell cycle phase and delayed apoptosis. Furthermore, the sorafenib-mediated suppression of immune effector cells, in particular the reduction of the CD8(+) T cell subset along with the down-regulation of key immune cell markers such as chemokine CC motif receptor 7 (CCR7), CD26, CD69, CD25, and CXCR3, was not observed in axitinib-treated immune effector cells. Therefore, axitinib rather than sorafenib seems to be suitable for implementation in complex treatment regimens of cancer patients including immunotherapy.
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更多信息

更多信息
物种 人类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 PBMC, 其它细胞系, 白细胞单采术样本, 白膜层, 脐带血
Selection Method Positive
法律声明:

Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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