EasySep™ Direct人嗜酸性粒细胞分选试剂盒

对来源于全血的未标记嗜酸性粒细胞进行免疫磁珠负选分离

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产品号 #（选择产品）

产品号 #19656_C

全血免疫磁珠负选试剂盒

产品优势

高效去除红细胞，无需密度梯度离心、沉降或裂解
分选获得的细胞纯度高达98%
操作简单、快捷，且无需分离柱
分选得到的细胞不带标记

产品组分包括

EasySep™ Direct人嗜酸性粒细胞分选试剂盒（产品号 #19656）

EasySep™ Direct人嗜酸性粒细胞分选抗体混合物，2 x 2.5 mL
EasySep™Direct RapidSpheres™磁珠50300，4 x 2.5 mL

立即询价

总览

使用 EasySep™ 直接人嗜酸性粒细胞分离试剂盒，可通过免疫磁性负选法直接从人全血样本中轻松高效地分离高纯度的嗜酸性粒细胞，无需密度梯度离心、沉降或红细胞裂解步骤。EasySep™ 技术在已发表研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。

在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物与名为 EasySep™ Direct RapidSpheres™ 的磁性颗粒进行标记。以下细胞将被去除：中性粒细胞、嗜碱性粒细胞、T 细胞、B 细胞、单核细胞、NK 细胞、树突状细胞、红系细胞和血小板。经 EasySep™ 磁体分离后，带有磁性标记的不需要细胞被去除，未标记的目标嗜酸性粒细胞通过倾倒或移液方式转移到新试管中。磁性分离完成后，目标嗜酸性粒细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括细胞表征、低温保存及其他相关产品。

实验数据

Figure 1. Typical EasySep™ Direct Human Eosinophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical eosinophil (CD16-CD66b+) content of the non-lysed final isolated fraction is 95.8 ± 2.7% (gated on CD45). In the above example, the eosinophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 2.3% and 97.6% (gated on CD45), respectively, or 2.2% and 90.8% (not gated on CD45), respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

文献 (1)

Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells. A. Haddad et al. Respiratory research 2019 oct

Abstract

BACKGROUND Asthma is a heterogenous disease characterized by chronic inflammation and airway remodeling. An increase in the severity of airway remodeling is associated with a more severe form of asthma. There is increasing interest in the epithelial to mesenchymal transition process and mechanisms involved in the differentiation and repair of the airway epithelium, especially as they apply to severe asthma. Growing evidence suggests that Epithelial-Mesenchymal transition (EMT) could contribute to airway remodeling and fibrosis in asthma. Severe asthmatic patients with remodeled airways have a neutrophil driven inflammation. Neutrophils are an important source of TGF-$\beta$1, which plays a role in recruitment and activation of inflammatory cells, extracellular matrix (ECM) production and fibrosis development, and is a potent inducer of EMT. OBJECTIVE As there is little data examining the contribution of neutrophils and/or their mediators to the induction of EMT in airway epithelial cells, the objective of this study was to better understand the potential role of neutrophils in severe asthma in regards to EMT. METHODS We used an in vitro system to investigate the neutrophil-epithelial cell interaction. We obtained peripheral blood neutrophils from severe asthmatic patients and control subjects and examined for their ability to induce EMT in primary airway epithelial cells. RESULTS Our data indicate that neutrophils from severe asthmatic patients induce changes in morphology and EMT marker expression in bronchial epithelial cells consistent with the EMT process when co-cultured. TGF-$\beta$1 levels in the culture medium of severe asthmatic patients were increased compared to that from co-cultures of non-asthmatic neutrophils and epithelial cells. CONCLUSIONS AND CLINICAL RELEVANCE As an inducer of EMT and an important source of TGF-$\beta$1, neutrophils may play a significant role in the development of airway remodeling and fibrosis in severe asthmatic airways.

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更多信息

更多信息
物种	人类
Magnet Compatibility	• “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源	全血
Selection Method	Negative

EasySep™ Direct人嗜酸性粒细胞分选试剂盒

产品号 #19656

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产品号 #19656_C

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产品组分包括

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实验数据

产品说明书及文档

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EasySep™ Direct人嗜酸性粒细胞分选试剂盒

产品号 #19656

产品号 #（选择产品）

产品号 #19656_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

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文献 (1)

Abstract

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