BrainPhys™ 神经元培养基N2-A和SM1试剂盒

用于在BrainPhys™神经元培养基中无血清培养ES/iPS细胞来源的神经元

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产品号 #05793_C

用于在BrainPhys™神经元培养基中无血清培养ES/iPS细胞来源的神经元

产品优势

更好地模拟大脑内的细胞外环境
增强神经元功能，获得更高比例的突触活性神经元
无需更换培养基并避免因此造成的细胞应激，即可进行功能性检测
支持由ES/iPS细胞及CNS来源的神经元的长期培养
严格的原材料筛选和质量控制可确保将批次间的差异性降至最低

产品组分包括

BrainPhys™神经元培养基，500 mL（产品号 #05790）
NeuroCult™ SM1神经元添加物，10 mL（产品号 #05711）
N2-A添加物，5 mL（产品号 #07152）

立即询价

总览

使用经过优化、旨在促进而非抑制神经元活性的完整培养基，对来源于人胚胎干细胞（ES）或诱导多能干细胞（iPS）的神经元进行培养、分化和成熟。

为方便使用，BrainPhys™神经元培养基N2-A和SM1试剂盒包含BrainPhys™神经元培养基（基础培养基）和用于培养人ES/iPS来源神经元祖细胞的添加物。基于Bardy和Gage的配方（Bardy et al. PNAS, 2015），无血清的BrainPhys™神经元培养基模拟了中枢神经系统（CNS）的细胞外环境，可获得更高比例的突触活性神经元。基于Brewer的B27配方（Brewer et al. J Neurosci Res., 1993）的NeuroCult™ SM1神经元添加物可在短期和长期无血清培养中维持细胞健康，并促进神经突起的生长和分支，而N2-A添加物则支持ES/iPS来源的细胞向神经元亚型的体外分化。若需进行依赖生长因子的谱系特异性分化，请参阅BrainPhys™ hPSC神经元试剂盒，该试剂盒还包含BDNF和GDNF。

为避免在更换培养基时对细胞造成应激，在进行诸如微电极阵列记录或活细胞荧光成像等功能性检测时，也可使用 BrainPhys™ 培养基。

查看我们的其他资源，了解更多关于BrainPhys™system的信息。

实验数据

Table 1. Properties of Culture Media (C Bardy et al. Proc Natl Acad Sci USA, 2015)

Check-mark denotes physiological conditions and supported activities according to C Bardy et al. Proc Natl Acad Sci USA, 2015.

Rodent Neurons Matured in BrainPhys™ Neuronal Medium

Figure 1. Protocol for Culturing hPSCs with the SM1 Culture System

hPSCs were maintained in mTeSR™1 medium and then differentiated using the STEMdiff™ SMADi Neural Induction Kit. Following plating on PLO/laminin, half-medium changes were performed to transition to BrainPhys™ Neuronal Medium for maturation and long-term culture.

hPSC-Derived Neurons Generated in BrainPhys™ Neuronal Medium and NeuroCult™ SM1 and N2 Supplements are Healthy and Morphologically Normal

Figure 2. hPSC-Derived Neurons Generated in BrainPhys™ Neuronal Medium and NeuroCult™ SM1 and N2 Supplements are Healthy and Morphologically Normal

NPCs were generated from H9 cells using STEMdiff™ Neural Induction Medium in an embryoid body-based protocol. Next, NPCs were cultured for 44 DIV in (A) BrainPhys™ Neuronal Medium, supplemented with 2% NeuroCult™ SM1 Supplement, 1% N2 Supplement-A, 20 ng/mL GDNF, 20 ng/mL BDNF, 1 mM db-cAMP and 200 nM ascorbic acid to initiate neuronal differentiation, or (B) DMEM/F12 under the same supplementation conditions. Neuronal cultures differentiated from NPCs in BrainPhys™ Neuronal Medium display extensive neurite outgrowth and reduced cellular debris compared to cultures differentiated in DMEM/F12. Scale bar= 100 µm.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

文献 (51)

Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs. E. Gabriel et al. Stem cell reports 2016 JAN

Abstract

Mitochondria are critical to neurogenesis,but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed differentiation of hiPSCs to DA neurons,mitochondria in these neurons exhibit pronounced changes during differentiation,including mature neurophysiology characterization and functional synaptic network formation. Inhibition of mitochondrial respiratory chains via application of complex IV inhibitor KCN (potassium cyanide) or complex I inhibitor rotenone restricted neurogenesis of DA neurons. These results demonstrated the direct importance of mitochondrial development and bioenergetics in DA neuronal differentiation. Our study also provides a neurophysiologic model of mitochondrial involvement in neurogenesis,which will enhance our understanding of the role of mitochondrial dysfunctions in neurodegenerative diseases.

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A viral strategy for targeting and manipulating interneurons across vertebrate species. Dimidschstein J et al. Nature neuroscience 2016 DEC

Abstract

A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical,physiological,cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular,it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species,including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust,allowing for morphological visualization,activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species,thus opening the possibility to study GABAergic function in virtually any vertebrate species.

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Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids. E. Gabriel et al. Cell stem cell 2017 JAN

Abstract

The recent Zika virus (ZIKV) epidemic is associated with microcephaly in newborns. Although the connection between ZIKV and neurodevelopmental defects is widely recognized,the underlying mechanisms are poorly understood. Here we show that two recently isolated strains of ZIKV,an American strain from an infected fetal brain (FB-GWUH-2016) and a closely-related Asian strain (H/PF/2013),productively infect human iPSC-derived brain organoids. Both of these strains readily target to and replicate in proliferating ventricular zone (VZ) apical progenitors. The main phenotypic effect was premature differentiation of neural progenitors associated with centrosome perturbation,even during early stages of infection,leading to progenitor depletion,disruption of the VZ,impaired neurogenesis,and cortical thinning. The infection pattern and cellular outcome differ from those seen with the extensively passaged ZIKV strain MR766. The structural changes we see after infection with these more recently isolated viral strains closely resemble those seen in ZIKV-associated microcephaly.

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