STEMmatrix™ Spike-In

成分明确、无须预包板的基质，用于在无血清、无饲养层条件下维持培养人多能干细胞

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成分明确、无须预包板的基质，用于在无血清、无饲养层条件下维持培养人多能干细胞

产品优势

无需培养板包被和预孵育步骤，节省时间
在铺板时直接加入培养基，或按批次配制并分装
可在室温下操作并在4°C下储存，无需解冻
使用重组人蛋白基质，减少实验变异性
可与TeSR™系列培养基及其他无饲养层和无血清的hPSC维持培养基配合使用

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概览

使用STEMmatrix™ Spike-In，一种可省去培养板包被和预孵育步骤的成分明确的基质，可在贴壁培养中实现对人多能干细胞（hPSCs）快速、简化的维持培养。在细胞接种过程中，将STEMmatrix™ Spike-In直接加入维持培养基中，可减少手动操作时间并简化常规工作流程。无需预先包被培养皿，STEMmatrix™ Spike-In在接种时提供Laminin-511片段，从而支持细胞在初始细胞-表面相互作用过程中通过整合素介导的细胞贴附。

STEMmatrix™ Spike-In为hPSC培养提供灵活的接种方案。您可以在接种时将其直接加入培养基中，也可以在铺板前与细胞及培养基混合制备成批次混合物。STEMmatrix™ Spike-In可在室温下操作而不会凝胶化，并可储存在4°C，免去了解冻步骤，减少了产品浪费，并提高了使用的便利性。

STEMmatrix™ Spike-In与无饲层和无血清的维持培养基（包括mTeSR™1、mTeSR™ Plus、TeSR™-E8™、TeSR™-AOF和eTeSR™）兼容，支持hPSC的常规维持培养和扩增，同时保留其多能性以及向外胚层、中胚层和内胚层分化的潜能。作为一种包含Laminin-511片段的成分明确基质，它可以精确控制培养环境，从而在下游应用中提供一致的细胞群和可重复的结果。与其他含Laminin-511片段的基质不同，STEMmatrix™ Spike-In经过优化，可与ReLeSR™或ACCUTASE™配合使用，以减少传代过程中细胞的长时间脱落。

实验数据

Figure 1. STEMmatrix™ Spike-In Enables Fast, Flexible Matrix Addition

To use STEMmatrix™ Spike-In, prepare the culture matrix according to the Product Information Sheet (PIS). No thawing of the matrix or pre-coating of culture plates is needed. The matrix can be added after the medium is added to the plate, followed by cell seeding (Stepwise Addition Protocol) or, alternatively, prepared as a mixture of medium, matrix, and cells, then aliquoted into the culture vessel (Batch Protocol). This streamlined workflow reduces total plating time to less than 2.5 minutes, representing a significant time saving compared to traditional matrix coating methods for human pluripotent stem cell (hPSC) culture.

Figure 2. STEMmatrix™ Spike-In Promotes High-Quality hPSC Morphology

Human induced pluripotent stem cells (hiPSCs; SCTi003-A) and human embryonic stem cells (hESCs; H9) cultured in mTeSR™ Plus, TeSR™-AOF, or eTeSR™ media with STEMmatrix™ Spike-In exhibit morphology consistent with high-quality, undifferentiated growth. In mTeSR™ Plus and TeSR™-AOF, cells form compact colonies with defined, smooth edges, while eTeSR™ cultures display a uniform, homogeneous monolayer morphology. Colony morphology appears well-defined and uniform relative to typical Corning® Matrigel®-based cultures.

Figure 3. STEMmatrix™ Spike-In Supports High Levels of Expansion Comparable to Corning® Matrigel®

Fold expansion of hPSCs (mean ± SEM, n = 5) cultured with STEMmatrix™ Spike-In or Corning® Matrigel® using mTeSR™ Plus or TeSR™-AOF media. Expansion values for mTeSR™ Plus and TeSR™-AOF were measured over 7-day aggregate passages. Within each media condition, STEMmatrix™ Spike-In consistently supported expansion comparable to Corning® Matrigel®, demonstrating reliable performance across multiple culture systems.

Figure 4. hPSCs Maintained on STEMmatrix™ Spike-In Retain Efficient Tri-Lineage Differentiation Capacity

Human iPSCs (WLS-1C) and hESCs (H1) were maintained using STEMmatrix™ Spike-In prior to and during directed differentiation. Cells efficiently differentiated into (A) ectoderm (PAX6⁺/Nestin⁺), (B) mesoderm (Brachyury⁺/NCAM⁺), and (C) endoderm (CXCR4⁺/SOX17⁺) lineages using the STEMdiff™ SMADi Neural Induction Kit, STEMdiff™ Mesoderm Induction Medium, and STEMdiff™ Definitive Endoderm Kit, respectively, confirming preservation of pluripotency and developmental potential following culture. Representative lineage-specific differentiation outcomes include (D) forebrain neurons generated using the STEMdiff™ Forebrain Neuron Differentiation Kit and (E) cardiomyocytes generated using the STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit following transfer to Corning® Matrigel®, as well as (F) hepatic progenitor-like cells generated using the STEMdiff™ Hepatocyte Kit with STEMmatrix™ Spike-In.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

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Product Information Sheet

Product Name

STEMmatrix™ Spike-In

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