EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #08571_C
用于人脑类器官成熟的培养基试剂盒
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系。
What Our Scientist Says
Human brain development is complex, so to observe it in a dish is a huge scientific breakthrough. We've tried to simplify that process and make it more accessible to you, regardless of how much stem cell experience you have.
Leon ChewScientist
总览
用STEMdiff™ 脑类器官成熟试剂盒培养多能干细胞（PSC）来源的神经类器官，可延长培养周期（＞40天）。
为方便使用，该成熟试剂盒与STEMdiff™ 脑类器官试剂盒完全兼容，后者可在明确的无血清条件下生成神经类器官。
如需了解STEMdiff™ 脑类器官成熟试剂盒培养类器官的实验方案，请查阅产品说明书（PIS）及教育资料。
有关使用 STEMdiff™ 脑类器官成熟试剂盒进行培养类器官的更多方案信息，请参阅产品说明书（PIS）及技术资料。
分类
专用培养基
细胞类型
神经细胞，PSC衍生，神经干/祖细胞，多能干细胞
种属
人
应用
细胞培养，鉴定，分化，功能学筛选，免疫荧光，类器官培养，表型鉴定，球状体培养
品牌
STEMdiff
研究领域
疾病建模，神经科学，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (42)
文献 (8)
Generation of human vascularized brain organoids
NeuroReport 2018
Abstract
The aim of this study was to vascularize brain organoids with a patient's own endothelial cells (ECs). Induced pluripotent stem cells (iPSCs) of one UC Davis patient were grown into whole-brain organoids. Simultaneously,iPSCs from the same patient were differentiated into ECs. On day 34,the organoid was re-embedded in Matrigel with 250 000 ECs. Vascularized organoids were grown in vitro for 3-5 weeks or transplanted into immunodeficient mice on day 54,and animals were perfused on day 68. Coating of brain organoids on day 34 with ECs led to robust vascularization of the organoid after 3-5 weeks in vitro and 2 weeks in vivo. Human CD31-positive blood vessels were found inside and in-between rosettes within the center of the organoid after transplantation. Vascularization of brain organoids with a patient's own iPSC-derived ECs is technically feasible.
Cerebral organoids at the air–liquid interface generate diverse nerve tracts with functional output
Nature Neuroscience 2019 apr
Abstract
Neural organoids have the potential to improve our understanding of human brain development and neurological disorders. However,it remains to be seen whether these tissues can model circuit formation with functional neuronal output. Here we have adapted air–liquid interface culture to cerebral organoids,leading to improved neuronal survival and axon outgrowth. The resulting thick axon tracts display various morphologies,including long-range projection within and away from the organoid,growth-cone turning,and decussation. Single-cell RNA sequencing reveals various cortical neuronal identities,and retrograde tracing demonstrates tract morphologies that match proper molecular identities. These cultures exhibit active neuronal networks,and subcortical projecting tracts can innervate mouse spinal cord explants and evoke contractions of adjacent muscle in a manner dependent on intact organoid-derived innervating tracts. Overall,these results reveal a remarkable self-organization of corticofugal and callosal tracts with a functional output,providing new opportunities to examine relevant aspects of human CNS development and disease.
Mechanisms of hyperexcitability in Alzheimer's disease hiPSC-derived neurons and cerebral organoids vs isogenic controls.
eLife 2019
Abstract
Human Alzheimer's disease (AD) brains and transgenic AD mouse models manifest hyperexcitability. This aberrant electrical activity is caused by synaptic dysfunction that represents the major pathophysiological correlate of cognitive decline. However,the underlying mechanism for this excessive excitability remains incompletely understood. To investigate the basis for the hyperactivity,we performed electrophysiological and immunofluorescence studies on hiPSC-derived cerebrocortical neuronal cultures and cerebral organoids bearing AD-related mutations in presenilin-1 or amyloid precursor protein vs. isogenic gene corrected controls. In the AD hiPSC-derived neurons/organoids,we found increased excitatory bursting activity,which could be explained in part by a decrease in neurite length. AD hiPSC-derived neurons also displayed increased sodium current density and increased excitatory and decreased inhibitory synaptic activity. Our findings establish hiPSC-derived AD neuronal cultures and organoids as a relevant model of early AD pathophysiology and provide mechanistic insight into the observed hyperexcitability.
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 无血清 |
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Legal Statement:
This product was developed under a license to intellectual property owned by the Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services or, in collaboration with, a for-profit entity, for purposes other than research applications (i.e., drug development activities). Purchasers wishing to use the product for commercial purposes should contact IMBA at technologvtransfer@imba.oeaw.ac.at.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.CN/COMPLIANCE.
Safety Statement:
CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov
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