EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒

免疫磁珠去除生物素化抗体标记的单一或多种小鼠非目的细胞类型

只有 %1 左

率先评论此产品

¥3,964.00

产品号 #（选择产品）

产品号 #19860_C

免疫磁珠去除生物素化抗体标记的单一或多种小鼠非目的细胞类型

产品优势

快速、简单
无需分离柱
获得的活细胞无标记

产品组分包括

EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒（产品号 #19860）

EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL

RoboSep™小鼠Streptavidin RapidSpheres™分选试剂盒（产品号 #19860RF）

EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL
RoboSep™空管
RoboSep™ 缓冲液（产品号 #20104）
RoboSep™ 过滤吸头（产品号 #20125）

立即询价

总览

EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒，通过免疫磁珠负选，可高效去除小鼠脾细胞或其他组织样本中被生物素化抗体标记的单一或多种非目的细胞类型。EasySep™技术结合单克隆抗体的特异性和无柱磁珠分选系统的简便性，已在发表的研究中广泛应用超过20年。

该优化的简易EasySep™流程，通过用生物素化抗体和链霉亲和素包被的磁珠（Streptavidin RapidSpheres™磁珠）标记非目的细胞。通过EasySep™磁极分离被标记的细胞，未标记的目的细胞被简单地倾倒出。分选后的细胞可立即用于下游应用，例如流式细胞术、培养或DNA/RNA提取。

不建议使用该试剂盒对小鼠细胞进行正选。如需正选，请使用EasySep™小鼠生物素正选试剂盒II（产品号 #17665）。

了解更多关于EasySep™免疫磁珠技术的工作原理，或如何使用RoboSep™实现免疫磁珠细胞分选全自动化。探索其他为您工作流程优化的产品，包括培养基、添加物、抗体等。

实验数据

Figure 1. Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Figure 2. Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Figure 3. Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

文献 (12)

Th2 and eosinophil responses suppress inflammatory arthritis. Chen Z et al. Nature communications 2016

Abstract

Th2-eosinophil immune responses are well known for mediating host defence against helminths. Herein we describe a function of Th2-eosinophil responses in counteracting the development of arthritis. In two independent models of arthritis,Nippostrongylus brasiliensis infection leads to Th2 and eosinophil accumulation in the joints associated with robust inhibition of arthritis and protection from bone loss. Mechanistically,this protective effect is dependent on IL-4/IL-13-induced STAT6 pathway. Furthermore,we show that eosinophils play a central role in the modulation of arthritis probably through the increase of anti-inflammatory macrophages into arthritic joints. The presence of these pathways in human disease is confirmed by detection of GATA3-positive cells and eosinophils in the joints of rheumatoid arthritis patients. Taken together,these results demonstrate that eosinophils and helminth-induced activation of the Th2 pathway axis effectively mitigate the course of inflammatory arthritis.

View publication

Alternative activation generates IL-10 producing type 2 innate lymphoid cells. C. R. Seehus et al. Nature communications 2017 DEC

Abstract

Type 2 innate lymphoid cells (ILC2) share cytokine and transcription factor expression with CD4+ Th2 cells,but functional diversity of the ILC2 lineage has yet to be fully explored. Here,we show induction of a molecularly distinct subset of activated lung ILC2,termed ILC210. These cells produce IL-10 and downregulate some pro-inflammatory genes. Signals that generate ILC210 are distinct from those that induce IL-13 production,and gene expression data indicate that an alternative activation pathway leads to the generation of ILC210. In vivo,IL-2 enhances ILC210 generation and is associated with decreased eosinophil recruitment to the lung. Unlike most activated ILC2,the ILC210 population contracts after cessation of stimulation in vivo,with maintenance of a subset that can be recalled by restimulation,analogous to T-cell effector cell and memory cell generation. These data demonstrate the generation of a previously unappreciated IL-10 producing ILC2 effector cell population.

View publication

Population snapshots predict early haematopoietic and erythroid hierarchies. B. K. Tusi et al. Nature 2018 FEB

Abstract

The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics,fate assays and a theory that allows the prediction of cell fates from population snapshots to demonstrate that mouse haematopoietic progenitors differentiate through a continuous,hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates,a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation,completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models,and provides insights into lineage development in vivo.

View publication

View All Publications

更多信息

更多信息
物种	小鼠
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源	其它细胞系, 脾脏
Selection Method	Depletion, Negative

EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒

产品号 #19860RF

产品号 #19860

产品号 #（选择产品）

产品号 #19860_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒

产品号 #19860RF

产品号 #19860

产品号 #（选择产品）

产品号 #19860_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

相关材料与文献

技术资料 (9)

文献 (12)

Abstract

Abstract

Abstract

Request Pricing

更多信息

欢迎您关注STEMCELL Technologies的微信公众平台