EasySep™小鼠CD8+ T细胞分选试剂盒

通过免疫磁珠负选分离无磁珠标记的小鼠CD8+ T细胞

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产品号 #（选择产品）

产品号 #19853_C

通过免疫磁珠负选分离无磁珠标记的小鼠CD8+ T细胞

产品优势

快速且易于使用
纯度高达95%
无需分离柱
获得无磁珠标记的活性细胞

产品组分包括

EasySep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853）

EasySep™小鼠CD8+ T细胞分选抗体混合物，0.5 mL
EasySep™ Streptavidin RapidSpheres™ 50001磁珠，2 x 1 mL
EasySep™小鼠FcR阻断剂，0.2 mL

RoboSep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853RF）

EasySep™小鼠CD8+ T细胞分选抗体混合物，0.5 mL
EasySep™ Streptavidin RapidSpheres™ 50001磁珠，2 x 1 mL
EasySep™小鼠FcR阻断剂，0.2 mL
RoboSep™ 缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）

立即询价

总览

使用EasySep™小鼠CD8+ T细胞分选试剂盒，可通过免疫磁珠负选技术，轻松高效地从脾细胞或其他组织的单细胞悬液中分离高纯度的小鼠CD8+ T细胞。EasySep™无柱免疫磁珠分选技术结合单克隆抗体的特异性和无需分选柱的简便性，20多年来被广泛引用于已发表的文献中。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。表达以下标志物的非目标细胞将被特异性去除：CD11b、CD45R、Ter119、CD4、CD49b、CD19、CD11c、TCRγδ和CD24。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需将目的细胞倾倒或吸取至一个新的试管中，仅需17.5分钟即可获得高纯度的CD8+ T细胞，且可立即用于流式细胞术、细胞培养及细胞实验等下游应用。

该产品可替代EasySep™人CD8+ T细胞富集试剂盒 (产品号 #19053) 以进行更快的细胞分选。

深入了解EasySep™免疫磁珠分选技术原理，或探索如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加物、抗体等。

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

常见问题 (7)

文献 (64)

The DNA damage response induces antigen presenting cell-like functions in fibroblasts Tang MLF et al. The European Journal of Immunology 2014

Abstract

The DNA damage response (DDR) alerts the immune system to the danger posed by DNA damage through the induction of damage-associated molecular pattern molecules,chemokines,and ligands for activating immune receptors such as lymphocyte function-associated antigen 1 (LFA-1),NKG2D,and DNAX accessory molecule 1 (DNAM-1). Here we provide evidence that OVA(257-264) -pulsed fibroblasts gain the ability to activate naïve OT-I CD8(+) T cells in response to DNA damage. The ability of fibroblasts to activate OT-I CD8(+) T cells depended on the upregulation of ICAM-1 on fibroblasts and DNAM-1 expression of CD8(+) T cells. OVA(257-264) -pulsed fibroblasts were able to induce a protective T-cell response against B16-OVA cells in a DDR-dependent manner. Hence,the DDR may alert the immune system to the presence of potentially dangerous cells by upregulating the expression of ligands that can induce the activation of innate and adaptive immune cells.

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Multiple Inflammatory Cytokines Converge To Regulate CD8+ T Cell Expansion and Function during Tuberculosis. Booty MG et al. Journal of Immunology 2016 FEB

Abstract

The differentiation of effector CD8(+) T cells is a dynamically regulated process that varies during different infections and is influenced by the inflammatory milieu of the host. In this study,we define three signals regulating CD8(+) T cell responses during tuberculosis by focusing on cytokines known to affect disease outcome: IL-12,type I IFN,and IL-27. Using mixed bone marrow chimeras,we compared wild-type and cytokine receptor knockout CD8(+) T cells within the same mouse following aerosol infection with Mycobacterium tuberculosis. Four weeks postinfection,IL-12,type 1 IFN,and IL-27 were all required for efficient CD8(+) T cell expansion in the lungs. We next determined if these cytokines directly promote CD8(+) T cell priming or are required only for expansion in the lungs. Using retrogenic CD8(+) T cells specific for the M. tuberculosis Ag TB10.4 (EsxH),we observed that IL-12 is the dominant cytokine driving both CD8(+) T cell priming in the lymph node and expansion in the lungs; however,type I IFN and IL-27 have nonredundant roles supporting pulmonary CD8(+) T cell expansion. Thus,IL-12 is a major signal promoting priming in the lymph node,but a multitude of inflammatory signals converge in the lung to promote continued expansion. Furthermore,these cytokines regulate the differentiation and function of CD8(+) T cells during tuberculosis. These data demonstrate distinct and overlapping roles for each of the cytokines examined and underscore the complexity of CD8(+) T cell regulation during tuberculosis.

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Low-Affinity Memory CD8+ T Cells Mediate Robust Heterologous Immunity. Krummey SM et al. Journal of Immunology 2016 MAR

Abstract

Heterologous immunity is recognized as a significant barrier to transplant tolerance. Whereas it has been established that pathogen-elicited memory T cells can have high or low affinity for cross-reactive allogeneic peptide-MHC,the role of TCR affinity during heterologous immunity has not been explored. We established a model with which to investigate the impact of TCR-priming affinity on memory T cell populations following a graft rechallenge. In contrast to high-affinity priming,low-affinity priming elicited fully differentiated memory T cells with a CD45RB(hi) status. High CD45RB status enabled robust secondary responses in vivo,as demonstrated by faster graft rejection kinetics and greater proliferative responses. CD45RB blockade prolonged graft survival in low affinity-primed mice,but not in high affinity-primed mice. Mechanistically,low affinity-primed memory CD8(+) T cells produced more IL-2 and significantly upregulated IL-2Rα expression during rechallenge. We found that CD45RB(hi) status was also a stable marker of priming affinity within polyclonal CD8(+) T cell populations. Following high-affinity rechallenge,low affinity-primed CD45RB(hi) cells became CD45RB(lo),demonstrating that CD45RB status acts as an affinity-based differentiation switch on CD8(+) T cells. Thus,these data establish a novel mechanism by which CD45 isoforms tune low affinity-primed memory CD8(+) T cells to become potent secondary effectors following heterologous rechallenge. These findings have direct implications for allogeneic heterologous immunity by demonstrating that despite a lower precursor frequency,low-affinity priming is sufficient to generate memory cells that mediate potent secondary responses against a cross-reactive graft challenge.

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物种	小鼠
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	其它细胞系, 脾脏
Selection Method	Negative

EasySep™小鼠CD8+ T细胞分选试剂盒

产品号 #19853

产品号 #19853RF

产品号 #（选择产品）

产品号 #19853_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

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Scientists Helping Scientists™

EasySep™小鼠CD8+ T细胞分选试剂盒

产品号 #19853

产品号 #19853RF

产品号 #（选择产品）

产品号 #19853_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (16)

常见问题 (7)

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Can I alter the separation time in the magnet?

文献 (64)

Abstract

Abstract

Abstract

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