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Easy50 EasySep™磁极

无柱免疫磁珠分选磁极

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产品号 #（选择产品）

产品号 #18002_C

无柱免疫磁珠分选磁极

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产品说明书及文档

应用领域

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使用 Easy 50 EasySep™ 磁极搭配 EasySep™ 细胞分选试剂盒，可轻松高效地进行细胞磁珠分选操作。Easy 50 EasySep™ 磁极在其内部腔体中生成高梯度磁场，足以分离标记有 EasySep™ 磁珠的细胞，无需使用分离柱。Easy 50 EasySep™ 磁体设计用于容纳标准 50 mL 锥形管，可在一次分选过程中处理多达 4 × 10⁹ 个细胞。

不确定该使用哪种磁极？请访问 EasySep™ 细胞分选磁极页面比较不同规格并选择适合您研究的磁极。

了解更多关于 EasySep™ 免疫磁性技术的工作原理。

种属
人，小鼠，非人灵长类，其他物种

应用
细胞分选

品牌
EasySep

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产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Easy 50 EasySep™ Magnet

Catalog #

18002

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for B Cell Research

Cell Sourcing

Cell Isolation

Cell Expansion

Cell Characterization

Workflow Stages for Dendritic Cell Research

Cell Sourcing

Cell Isolation

Cell Differentiation and Maturation

Cell Characterization

Workflow Stages for Granulocyte Research

Cell Sourcing

Cell Isolation

Cell Characterization

Workflow Stages for Total Lymphocyte Research

Cell Sourcing

Cell Isolation

Cell Characterization

Workflow Stages for Monocyte Research

Cell Sourcing

Cell Isolation

Cell Differentiation and Maturation

Cell Characterization

Workflow Stages for Myeloid Cell Research

Cell Sourcing

Cell Isolation

Cell Differentiation & Maturation

Cell Characterization

Workflow Stages for Natural Killer Cell Research

Cell Sourcing

Cell Isolation

Cell Expansion and Differentiation

Cell Characterization

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Workflow Stages for HLA Analysis

Cell Isolation

Characterization

Workflow Stages for T Cell Engineering

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Cell Cryopreservation

Workflow Stages for Chimerism Analysis

Cell Isolation

Characterization

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Which cell separation kits are compatible with the "Easy 50" EASYSEP™ magnet?

At present, the "Easy 50" EasySep™ magnet is only compatible with EasySep™ kits for human cell separation.

T Cells: 19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19159 (Memory CD8 T Cells - please contact Tech Support)

B Cells: 19054 (B Cells), 19254 (Naïve B cells)

Other Cell Types: 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes), 19062 (Plasmacytoid DCs), 19251 (pan-DCs)

For HLA Analysis: 19951HLA (T Cells from whole blood), 19954HLA (B Cells from whole blood), 19961HLA (Total lymphocytes from whole blood)

Easy50 EasySep™磁极

产品号 #18002

产品号 #（选择产品）

产品号 #18002_C

总览

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

Easy50 EasySep™磁极

产品号 #18002

产品号 #（选择产品）

产品号 #18002_C

总览

产品说明书及文档

应用领域

相关材料与文献

技术资料 (5)

常见问题

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

Which cell separation kits are compatible with the "Easy 50" EASYSEP™ magnet?

Request Pricing

更多信息

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