总览

使用 EasySep™ 大鼠 CD8⁺ T 细胞分离试剂盒，可通过免疫磁性负选法从大鼠脾细胞、淋巴结、全血或其他组织的单细胞悬液中轻松高效地分离高纯度的 CD8⁺ T 细胞。EasySep™ 技术在已发表研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。

在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物与磁性颗粒进行标记。以下细胞将被去除：CD4⁺ T 细胞亚群、B 细胞、NK 细胞、单核细胞、粒细胞和树突状细胞。经 EasySep™ 磁体分离后，带有磁性标记的不需要细胞被去除，未标记的目标 CD8⁺ T 细胞通过倾倒或移液方式转移至新试管中。磁性细胞分离完成后，目标 CD8⁺ T 细胞可直接用于流式细胞术、细胞培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁性技术的工作原理，或了解如何使用 RoboSep™ 实现免疫磁性细胞分离的全自动化。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• Easy 50 EasySep™磁极（产品号 #18002）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）

分类
细胞分选试剂盒

细胞类型
T 细胞，T 细胞，CD8+

种属
大鼠

样本来源
其他物种，脾脏，全血

分选方法
负选

应用
细胞分选

品牌
EasySep，RoboSep

研究领域
免疫

查看更多显示更少

实验数据

Figure 1. Typical EasySep™ Rat CD8+ T Cell Isolation Kit Profile

Starting with rat splenocytes, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 91.5 ± 3.0% (mean ± SD using the purple EasySep™ Magnet).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Sample Preparation

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

物种	大鼠
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	全血, 其它细胞系, 脾脏
Selection Method	Negative

EasySep™大鼠CD8+ T细胞分选试剂盒

产品号 #19643

产品号 #19643RF

产品号 #（选择产品）

产品号 #19643_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

EasySep™大鼠CD8+ T细胞分选试剂盒

产品号 #19643

产品号 #19643RF

产品号 #（选择产品）

产品号 #19643_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (8)

常见问题

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Request Pricing

更多信息

欢迎您关注STEMCELL Technologies的微信公众平台