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EasySep™小鼠Pan-DC富集试剂盒II

从小鼠脾细胞或其他组织样本的单细胞悬液中，通过免疫磁珠负选法分离出无磁珠标记的小鼠树突状细胞（DCs），包括常规树突状细胞和浆细胞样树突状细胞。

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产品号 #（选择产品）

产品号 #19863_C

免疫磁珠负选试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达65%
  • 分选得到的细胞不带标记
  • 为高得率进行优化
  • 便于快速流式分选DC亚型

产品组分包括

  • EasySep™小鼠Pan-DC富集试剂盒（产品号 #19863）
    • EasySep™小鼠Pan-DC富集抗体混合物II，1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

c总览

使用 EasySep™ 小鼠全树突状细胞富集试剂盒 II，可通过免疫磁珠负选法从小鼠脾细胞或其他组织样本的单细胞悬液中轻松高效地分离高纯度的小鼠树突状细胞（DCs），包括常规型和浆细胞样DCs。EasySep™ 技术在已发表研究中被广泛使用超过 20 年，其结合了单克隆抗体的特异性与无柱磁珠分选系统的简便性。

在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物与磁珠进行标记。表达以下标志物的细胞将被去除：CD2、CD22、Ter119、CD19、7/4、CD43、F4/80 和 CD90.2。经 EasySep™ 磁极分离后，带有磁珠标记的非目的细胞被去除，未标记的目标 DC 细胞通过倾倒或移液方式转移到新试管中。磁珠分选完成后，目标 DC 细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁珠技术的工作原理，或了解如何使用 RoboSep™ 实现免疫磁珠细胞分选的全自动化。探索更多优化实验流程的产品，包括培养基、添加物、抗体等。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
树突状细胞（DCs）
 
种属
小鼠
 
样本来源
脾脏
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫，感染性疾病(传染病)
 

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实验数据

Flow Cytometry Profile Results with EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
产品说明书
Product Name
RoboSep™ 小鼠Pan-DC富集试剂盒II
Catalog #
19863RF
Lot #
All
Language
中文
Document Type
产品说明书
Product Name
EasySep™小鼠Pan-DC富集试剂盒II
Catalog #
19863
Lot #
All
Language
中文
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Pan-DC Enrichment Kit II
Catalog #
19863RF
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (7)

Cell Enrichment Prior to Cell Sorting
产品手册
Cell Enrichment Prior to Cell Sorting
EasySep™细胞分选技术 - 更高效地分离细胞
产品手册
EasySep™细胞分选技术 - 更高效地分离细胞
Mouse Immune Cell Isolation Course
点播课程
Mouse Immune Cell Isolation Course
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
视频
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Prepare a Single-Cell Suspension from Mouse Spleen
视频
How to Prepare a Single-Cell Suspension from Mouse Spleen
Frequencies and Percentages of Mouse Immune Cell Types
挂图
Frequencies and Percentages of Mouse Immune Cell Types
Antigen Processing and Presentation
挂图
Antigen Processing and Presentation

文献 (1)

Adjuvant potential of Peyssonnelia caulifera extract on the efficacy of an influenza vaccine in a murine model Scientific Reports 2024 Oct

Abstract

Natural adjuvants have recently garnered interest in the field of vaccinology as their immunostimulatory effects. In this study,we aimed to investigate the potential use of Peyssonnelia caulifera (PC),a marine alga,as a natural adjuvant for an inactivated split A/Puerto Rico/8/1934 H1N1 influenza vaccine (sPR8) in a murine model. We administered PC-adjuvanted vaccines to a murine model via intramuscular prime and boost vaccinations,and subsequently analyzed the induced immunological responses,particularly the production of antigen-specific IgG1 and IgG2a antibodies,memory T and B cell responses,and the protective efficacy against a lethal viral infection. PC extract significantly bolstered the vaccine efficacy,demonstrating balanced Th1/Th2 responses,increased memory T and B cell activities,and improved protection against viral infection. Notably,within 3 days post-vaccination,the PC adjuvant stimulated activation markers on dendritic cells (DCs) and macrophages at the inguinal lymph nodes (ILN),emphasizing its immunostimulatory capabilities. Furthermore,the safety profile of PC was confirmed,showing minimal local inflammation and no significant adverse effects post-vaccination. These findings contribute to our understanding of the immunomodulatory properties of natural adjuvants and suggest the promising roles of natural adjuvants in the development of more effective vaccines for infectious diseases.Supplementary InformationThe online version contains supplementary material available at 10.1038/s41598-024-76736-9.
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更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 脾脏
Selection Method Negative
标记抗体
  1. 抗小鼠CD19抗体，clone ID3
    抗小鼠CD19抗体，clone ID3

    大鼠Monoclonal IgG2a抗体，抗小鼠CD19

  2. 抗小鼠CD3e抗体，clone 145-2C11
    抗小鼠CD3e抗体，clone 145-2C11

    亚美尼亚仓鼠Monoclonal IgG1抗体，抗小鼠CD3e

  3. 抗小鼠F4/80抗体，clone BM8
    抗小鼠F4/80抗体，clone BM8

    大鼠Monoclonal IgG2a抗体，抗小鼠F4/80

  4. 抗小鼠Ly-6G抗体，clone 1A8
    抗小鼠Ly-6G抗体，clone 1A8

    大鼠Monoclonal IgG2a抗体，抗小鼠Ly-6G

  5. 抗小鼠NK1.1（CD161）抗体，clone PK136
    抗小鼠NK1.1（CD161）抗体，clone PK136

    抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交Monoclonal IgG2a抗体

  6. 抗小鼠TER119抗体，clone TER-119
    抗小鼠TER119抗体，clone TER-119

    抗小鼠TER119的大鼠（WI）Monoclonal IgG2b抗体

  7. 抗小鼠CD11c抗体，clone N418
    抗小鼠CD11c抗体，clone N418

    抗小鼠CD11c的仓鼠（亚美尼亚）Monoclonal IgG抗体

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