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EasySep™小鼠造血祖细胞分选试剂盒

通过免疫磁珠负选分离出无磁珠标记的小鼠干细胞与祖细胞
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产品号 #（选择产品）

产品号 #19856_C

通过免疫磁珠负选分离出无磁珠标记的小鼠干细胞与祖细胞

产品优势

  • 易于操作、快速
  • 纯度高达84%
  • 无需分离柱

产品组分包括

  • EasySep™小鼠造血祖细胞分选试剂盒（产品号 #19856）
    • EasySep™小鼠造血祖细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
    • EasySep™ 小鼠FcR阻断剂（产品号#18730），0.2 mL
  • RoboSep™小鼠造血祖细胞分选试剂盒产品号 #19856RF）
    • EasySep™小鼠造血祖细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
    • EasySep™ 小鼠FcR阻断剂（产品号#18730），0.2 mL
    • RoboSep™ 缓冲液（产品号#20104）
    • RoboSep™过滤吸头（产品号#20125）
立即询价
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠造血祖细胞分选试剂盒，通过免疫磁珠负选技术，可轻松高效地从骨髓样本中分离高纯度小鼠祖细胞。当使用其他类型组织来源的单细胞悬液时，该试剂盒可能需要优化。

EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在此 EasySep™ 负选方案中，非目的细胞通过抗体复合物与磁珠标记。非目的细胞可通过针对非造血干细胞和非祖细胞（CD5、CD11b、CD19、CD45R/B220、Ly6G/C（Gr-1）、TER119、7-4）的抗体而被去除。通过使用 EasySep™ 磁力架，将被磁珠标记的非目的细胞分离，仅需简单倒出，即可获得无磁珠标记的目标小鼠造血细胞。完成磁珠分选后，目标小鼠造血细胞可直接用于流式细胞术、细胞培养等下游实验。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
小鼠
 
样本来源
骨髓
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫，干细胞生物学
 

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实验数据

Typical EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Profile

Starting with a mouse bone marrow cell suspension, the lineage antigen-negative cell content of the isolated fraction typically ranges from 60 - 84%.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
1000138480 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
1000138481 or higher
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
1000138480 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
1000138481 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit
Catalog #
19856
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (5)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Mouse Cell Isolation
Brochure
Mouse Cell Isolation
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Mouse Immune Cell Isolation Course
On-Demand Training
Mouse Immune Cell Isolation Course

常见问题 (11)

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (17)

EMP1 safeguards hematopoietic stem cells by suppressing sphingolipid metabolism and alleviating endoplasmic reticulum stress Nature Communications 2025 Jul

Abstract

The long-term maintenance of hematopoietic stem cells (HSCs) relies on the regulation of endoplasmic reticulum (ER) stress at a low level, but the underlying mechanism remains poorly understood. Here, we demonstrate that suppression of ER stress improves the functions of HSCs and protects HSCs against ionizing radiation (IR)-induced injury. We identify epithelial membrane protein 1 (EMP1) as a key regulator that mitigates ER stress in HSCs. Emp1 deficiency leads to the accumulation of protein aggregates and elevated ER stress, ultimately resulting in impaired HSC maintenance and self-renewal. Mechanistically, EMP1 is located within the ER and interacts with ceramide synthase 2 (CERS2) to limit the production of a class of sphingolipids, dihydroceramides (dhCers). DhCers accumulate in Emp1-deficient HSCs and induce protein aggregation. Furthermore, Emp1 deficiency renders HSCs more susceptible to IR, while overexpression of Emp1 or inhibition of CERS2 protects HSCs against IR-induced injury. These findings highlight the critical role played by the EMP1-CERS2-dhCers axis in constraining ER stress and preserving HSC potential. A new study shows EMP1 protects hematopoietic stem cells by suppressing sphingolipid metabolism and ER stress. EMP1 interacts with CERS2 to limit dihydroceramide production, which causes protein aggregation when elevated.
View publication
GATA2 mutated allele specific expression is associated with a hyporesponsive state of HSC in GATA2 deficiency syndrome Blood Cancer Journal 2025 Jan

Abstract

GATA2 germline mutations lead to a syndrome characterized by immunodeficiency, vascular disorders and myeloid malignancies. To elucidate how these mutations affect hematopoietic homeostasis, we created a knock-in mouse model expressing the recurrent Gata2 R396Q missense mutation. Employing molecular and functional approaches, we investigated the mutation’s impact on hematopoiesis, revealing significant alterations in the hematopoietic stem and progenitor (HSPC) compartment in young age. These include increased LT-HSC numbers, reduced self-renewal potential, and impaired response to acute inflammatory stimuli. The mature HSPC compartment was primarily affected at the CMP sub-population level. In the mutant LT-HSC population, we identified an aberrant subpopulation strongly expressing CD150, resembling aging, but occurring prematurely. This population showed hyporesponsiveness, accumulated over time, and exhibited allele-specific expression (ASE) favoring the mutated Gata2 allele, also observed in GATA2 mutated patients. Our findings reveal the detrimental impact of a Gata2 recurrent missense mutation on the HSC compartment contributing to its functional decline. Defects in the CMP mature compartment, along with the inflammatory molecular signature, explain the loss of heterogeneity in HPC compartment observed in patients. Finally, our study provides a valuable model that recapitulates the ASE-related pathology observed in GATA2 deficiency, shedding light on the mechanisms contributing to the disease’s natural progression.
View publication
The chromatin reader Dido3 is a regulator of the gene network that controls B cell differentiation Cell & Bioscience 2025 Apr

Abstract

The development of hematopoietic cell lineages is a highly complex process governed by a delicate interplay of various transcription factors. The expression of these factors is influenced, in part, by epigenetic signatures that define each stage of cell differentiation. In particular, the formation of B lymphocytes depends on the sequential silencing of stemness genes and the balanced expression of interdependent transcription factors, along with DNA rearrangement. We have investigated the impact of Dido3 deficiency, a protein involved in chromatin status readout, on B cell differentiation within the hematopoietic compartment of mice. Our findings revealed significant impairments in the successive stages of B cell development. The absence of Dido3 resulted in remarkable alterations in the expression of essential transcription factors and differentiation markers, which are crucial for orchestrating the differentiation process. Additionally, the somatic recombination process, responsible for generation of antigen receptor diversity, was also adversely affected. These observations highlight the vital role of epigenetic regulation, particularly the involvement of Dido3, in ensuring proper B cell differentiation. This study reveals new mechanisms underlying disruptive alterations, deepening our understanding of hematopoiesis and may potentially lead to insights that aid in the development of therapeutic interventions for disorders involving aberrant B cell development.Supplementary InformationThe online version contains supplementary material available at 10.1186/s13578-025-01394-x.
View publication
View All Publications

更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源 骨髓
Selection Method Negative
标记抗体
  1. 抗小鼠CD11b抗体，clone M1/70
    抗小鼠CD11b抗体，clone M1/70

    抗人、小鼠、恒河猴CD11b的大鼠Monoclonal IgG2b抗体

  2. 抗CD19抗体，clone 6D5
    抗CD19抗体，clone 6D5

    抗小鼠CD19的大鼠Monoclonal IgG2a抗体

  3. 抗小鼠CD3e抗体，clone 145-2C11
    抗小鼠CD3e抗体，clone 145-2C11

    亚美尼亚仓鼠Monoclonal IgG1抗体，抗小鼠CD3e

  4. 抗小鼠CD45R抗体，clone RA3-6B2
    抗小鼠CD45R抗体，clone RA3-6B2

    大鼠Monoclonal IgG2a抗体，抗人、小鼠、猫CD45R（B220）

  5. 抗小鼠Ly-6G抗体，clone 1A8
    抗小鼠Ly-6G抗体，clone 1A8

    大鼠Monoclonal IgG2a抗体，抗小鼠Ly-6G

  6. 抗小鼠Sca1抗体，clone E13-161.7
    抗小鼠Sca1抗体，clone E13-161.7

    大鼠抗小鼠Sca1 (Ly-6A/E)Monoclonal IgG2a抗体

  7. 抗小鼠TER119抗体，clone TER-119
    抗小鼠TER119抗体，clone TER-119

    抗小鼠TER119的大鼠（WI）Monoclonal IgG2b抗体

  8. 抗小鼠CD117抗体，clone 2B8
    抗小鼠CD117抗体，clone 2B8

    大鼠Monoclonal IgG2b抗体，抗小鼠CD117（c-Kit）

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