总览

使用 EasySep™ 小鼠 CD90.1 正选试剂盒，可通过免疫磁性正选法从小鼠脾细胞、淋巴结或外周血样本中轻松分离高纯度的小鼠 CD90.1⁺（Thy1.1⁺）细胞。EasySep™ 技术在发表研究中已被广泛使用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。

在此 EasySep™ 正选步骤中，目标细胞通过识别 CD90.1 的抗体复合物与磁性颗粒进行标记。经 EasySep™ 磁极分离后，不需要的细胞被倒出或移除，目标细胞保留在管中。磁性细胞分离完成后，获得的 CD90.1⁺（Thy1.1⁺）细胞可直接用于流式细胞术、细胞培养或基于细胞的实验。

了解更多关于 EasySep™ 免疫磁性细胞分选技术的工作原理，或了解如何使用 RoboSep™ 实现免疫磁性细胞分离的全自动化。探索更多产品，包括培养基、补充物、抗体等，以优化您的实验流程。

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyPlate™ EasySep™磁极（产品号 #18102）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）

分类
细胞分选试剂盒

细胞类型
T 细胞

种属
小鼠

样本来源
其他物种，脾脏

分选方法
正选

应用
细胞分选

品牌
EasySep，RoboSep

研究领域
免疫

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实验数据

Figure 1. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD; using the purple EasySep™ Magnet).

Figure 2. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Starting with mouse splenocytes from adoptive lymphocyte transfer samples, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD; using the purple EasySep™ Magnet).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

RoboSep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958RF

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

EasySep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Mouse CD90.1 Positive Selection Kit

Catalog #

18958

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Sample Preparation

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Serotonin receptor 5-HT2A as a potential target for HCC immunotherapy Journal for Immunotherapy of Cancer 2025 Jun

Abstract

AbstractBackgroundWhile recent clinical trials of combination immunotherapies for hepatocellular carcinoma (HCC) have shown promising clinical efficacy and survival improvements breakthroughs, there is still much room for further improvement. A key limiting factor for HCC immunotherapy is the intrinsic immunosuppression within the liver microenvironment, resulting in suboptimal priming of tumor-specific CD8 cytotoxic T cells and thus immune evasion by the tumor. Hence, identifying new key molecular pathways suppressing T-cell responses within the liver is critical for the rational design of more effective combination immunotherapies for HCC.MethodsWe identified the 5-HT2A serotonin receptor as a potential target for HCC immunotherapy in a chemical screening approach and validated that targeting 5-HT2A signaling could be a viable approach for HCC immunotherapy via in vitro and in vivo studies.ResultsDisruption of 5-HT2A signaling using either a selective antagonist small molecule, ketanserin, or by knockout of its coding gene Htr2a augments the cytotoxic effector phenotype of mouse CD8 T cells activated in vitro with immunosuppressive liver non-parenchymal cells. Ketanserin treatment of in vitro activated human CD8 T cells also increased expression of the cytotoxic effector molecules granzyme B and perforin. Abrogation of 5-HT2A signaling was associated with increased expression of cytotoxicity-related genes such as granzyme B and reduced expression of transcription factors downstream of MAP kinase signaling. In vivo, systemic ketanserin treatment significantly prolonged survival of HCC tumor-bearing mice and was non-inferior to α-programmed death ligand 1 (PD-L1)+α-vascular endothelial growth factor A (VEGFA) combination antibody treatment. Combining ketanserin with αPD-L1+αVEGFA antibodies also significantly prolonged survival relative to control-treated mice while preserving the occurrence of complete tumor regression observed with αPD-L1+αVEGFA treatment alone.ConclusionsTogether, our data describe a role for 5-HT2A as a negative regulator of the cytotoxic effector phenotype in CD8 T cells and highlight the therapeutic potential of targeting 5-HT2A for HCC immunotherapy.

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iPSC-derived trimodal T cells engineered with CAR, TCR, and hnCD16 modalities can overcome antigen escape in heterogeneous tumors Cell Reports Medicine 2025 Jun

Abstract

SummaryAlthough chimeric antigen receptor (CAR) T cells have demonstrated therapeutic activity in hematopoietic malignancies, tumor heterogeneity has impeded the efficacy of CAR T cells and their extension into successful solid tumor treatment. To address these challenges, induced pluripotent stem cell (iPSC)-derived T (iT) cells are engineered to uniformly express CAR and T cell receptor (TCR), enabling targeting of both surface and intracellular antigens, respectively, along with a high-affinity, non-cleavable variant of CD16a (hnCD16) to support antibody-dependent cellular cytotoxicity (ADCC) when combined with therapeutic antibodies. Co-expression of each antitumor strategy on engineered iT cells enables independent and antigen-specific targeting across a diverse set of liquid and solid tumors. In heterogeneous tumor models, coactivation of these modalities is required for measurable antitumor efficacy, with activation of all three modalities displaying maximal efficacy. These data highlight the therapeutic potential of an off-the-shelf engineered iPSC-derived trimodal T cell expressing CAR, TCR, and hnCD16 to combat difficult-to-treat heterogeneous tumors. Graphical abstract Highlights•CAR, TCR, and hnCD16 can be uniformly co-expressed and can function in iT cells•hnCD16 signals through CD3ζ and arms iT cells with targeting flexibility through ADCC•Concurring CAR, TCR, and hnCD16 activation demonstrates a cooperative effect•Multi-targeting with trimodal iT cells can control heterogeneous tumors in vivo Yang et al. show that (1) trimodal iPSC cells expressing CAR, TCR, and hnCD16 can commit to T cell lineage, (2) hnCD16 signals through CD3ζ in iT cells and arms iT cells with ADCC targeting flexibility, and (3) trimodal iT cells control antigen-heterogeneous tumors in vivo through multi-modal targeting.

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EasySep™小鼠CD90.1正选试剂盒

产品号 #18958RF

产品号 #18958

产品号 #（选择产品）

产品号 #18958_C

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Scientists Helping Scientists™

物种	小鼠
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™- S (Catalog #21000)
样本来源	其它细胞系, 脾脏
Selection Method	Positive

EasySep™小鼠CD90.1正选试剂盒

产品号 #18958RF

产品号 #18958

产品号 #（选择产品）

产品号 #18958_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (10)

常见问题 (11)

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

文献 (2)

Abstract

Abstract

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