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EasySep™小鼠CD49b正选试剂盒

对来源于小鼠脾细胞或其他单细胞悬液的 CD49b⁺ 细胞进行免疫磁珠正选分离

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产品号 #（选择产品）

产品号 #18755_C

免疫磁珠正选小鼠CD49b细胞

产品优势

  • 操作简单、快速
  • 纯度高达90%
  • 无需分离柱

产品组分包括

  • EasySep™小鼠CD49b正选试剂盒（产品号 #18755）
    • EasySep™小鼠CD49b PE标记试剂，1 mL
    • EasySep™PE分选抗体混合物，2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
  • RoboSep™ 小鼠CD49b正选试剂盒（含过滤枪头）（产品号 #18755RF）
    • EasySep™小鼠CD49b PE标记试剂，1 mL
    • EasySep™PE分选抗体混合物，2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤枪头（产品号 #20125）x 2
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  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用 EasySep™ 小鼠 CD49b 正选试剂盒，可通过免疫磁珠正选法从小鼠脾细胞或其他单细胞悬液中高效分离高纯度的 CD49b⁺ 细胞。EasySep™ 技术在发表研究中已被广泛使用超过 20 年，其结合了单克隆抗体的特异性与无柱磁珠分选系统的简便性。

在此 EasySep™ 正选步骤中，目标细胞通过识别 CD49b 的抗体复合物和磁珠进行标记。经 EasySep™ 磁极分离后，非目的细胞被倒出或移除， CD49b⁺ 目的细胞保留在管中。磁珠分选后，CD49b⁺ 细胞可直接用于流式细胞术、细胞培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁珠细胞分选技术的工作原理，或了解如何使用 RoboSep™ 实现全自动化免疫磁珠细胞分选。探索更多产品以优化您的实验流程，包括培养基、添加物、抗体等。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
NK 细胞
 
种属
小鼠
 
样本来源
其它组织，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

FACS histogram showing highly purified mouse CD49b+ cells following cell isolation with the EasySep™ Mouse CD49b Positive Selection Kit.

Figure 1. EasySep™ Mouse CD49b Positive Selection Kit Yields Highly Purified CD49b+ Cells

Starting with mouse splenocytes, the CD49b+ cell content of the isolated fraction typically ranges from 74 to 90%. In the above example, the purities of the start and final isolated fractions are 6.2% and 90.2%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
产品说明书
Product Name
RoboSep™ 小鼠CD49b正选试剂盒含滤芯吸头
Catalog #
18755RF
Lot #
All
Language
中文
Document Type
产品说明书
Product Name
EasySep™小鼠CD49b正选试剂盒
Catalog #
18755
Lot #
All
Language
中文
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse CD49b Positive Selection Kit
Catalog #
18755
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse CD49b Positive Selection Kit with Filter Tips
Catalog #
18755RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD49b Positive Selection Kit
Catalog #
18755
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD49b Positive Selection Kit
Catalog #
18755
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse CD49b Positive Selection Kit
Catalog #
18755
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD49b Positive Selection Kit with Filter Tips
Catalog #
18755RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD49b Positive Selection Kit with Filter Tips
Catalog #
18755RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse CD49b Positive Selection Kit with Filter Tips
Catalog #
18755RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse CD49b Positive Selection Kit with Filter Tips
Catalog #
18755RF
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (7)

EasySep™细胞分选技术 - 更高效地分离细胞
产品手册
EasySep™细胞分选技术 - 更高效地分离细胞
Mouse Immune Cell Isolation Course
点播课程
Mouse Immune Cell Isolation Course
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
视频
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
视频
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Frequencies and Percentages of Mouse Immune Cell Types
挂图
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
挂图
Human Immune Cytokines
Antigen Processing and Presentation
挂图
Antigen Processing and Presentation

常见问题 (11)

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (6)

Cutting edge: murine dendritic cells require IL-15R alpha to prime NK cells. Koka R et al. Journal of immunology (Baltimore,Md. : 1950) 2004 SEP

Abstract

NK cells protect hosts against viral pathogens and transformed cells,and dendritic cells (DCs) play important roles in activating NK cells. We now find that murine IL-15Ralpha-deficient DCs fail to support NK cell cytolytic activity and elaboration of IFN-gamma,despite the fact that these DCs express normal levels of costimulatory molecules and IL-12. By contrast,IL-15Ralpha expression on NK cells is entirely dispensable for their activation by DCs. In addition,blockade with anti-IL-15Ralpha and anti-IL-2Rbeta but not anti-IL-2Ralpha-specific Abs prevents NK cell activation by wild-type DCs. Finally,presentation of IL-15 by purified IL-15Ralpha/Fc in trans synergizes with IL-12 to support NK cell priming. These findings suggest that murine DCs require IL-15Ralpha to present IL-15 in trans to NK cells during NK cell priming.
View publication
Contribution of Hfe expression in macrophages to the regulation of hepatic hepcidin levels and iron loading. Makui H et al. Blood 2005 SEP

Abstract

Hereditary hemochromatosis (HH),an iron overload disease associated with mutations in the HFE gene,is characterized by increased intestinal iron absorption and consequent deposition of excess iron,primarily in the liver. Patients with HH and Hfe-deficient (Hfe-/-) mice manifest inappropriate expression of the iron absorption regulator hepcidin,a peptide hormone produced by the liver in response to iron loading. In this study,we investigated the contribution of Hfe expression in macrophages to the regulation of liver hepcidin levels and iron loading. We used bone marrow transplantation to generate wild-type (wt) and Hfe-/- mice chimeric for macrophage Hfe gene expression. Reconstitution of Hfe-deficient mice with wt bone marrow resulted in augmented capacity of the spleen to store iron and in significantly decreased liver iron loading,accompanied by a significant increase of hepatic hepcidin mRNA levels. Conversely,wt mice reconstituted with Hfe-deficient bone marrow had a diminished capacity to store iron in the spleen but no significant alterations of liver iron stores or hepcidin mRNA levels. Our results suggest that macrophage Hfe participates in the regulation of splenic and liver iron concentrations and liver hepcidin expression.
View publication
Activating Ly-49 receptors regulate LFA-1-mediated adhesion by NK cells. Osman MS et al. Journal of immunology (Baltimore,Md. : 1950) 2007 FEB

Abstract

NK cells are important for innate resistance to tumors and viruses. Engagement of activating Ly-49 receptors expressed by NK cells leads to rapid NK cell activation resulting in target cell lysis and cytokine production. The ITAM-containing DAP12 adapter protein stably associates with activating Ly-49 receptors,and couples receptor recognition with generation of NK responses. Activating Ly-49s are potent stimulators of murine NK cell functions,yet how they mediate such activities is not well understood. We demonstrate that these receptors trigger LFA-1-dependent tight conjugation between NK cells and target cells. Furthermore,we show that activating Ly-49 receptor engagement leads to rapid DAP12-dependent up-regulation of NK cell LFA-1 adhesiveness to ICAM-1 that is also dependent on tyrosine kinases of the Syk and Src families. These results indicate for the first time that activating Ly-49s control adhesive properties of LFA-1,and by DAP12-dependent inside-out signaling. Ly-49-driven mobilization of LFA-1 adhesive function may represent a fundamental proximal event during NK cell interactions with target cells involving activating Ly-49 receptors,leading to target cell death.
View publication
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更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源 其它细胞系, 脾脏
Selection Method Positive
标记抗体
  1. 抗小鼠NK1.1（CD161）抗体，clone PK136
    抗小鼠NK1.1（CD161）抗体，clone PK136

    抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交Monoclonal IgG2a抗体

  2. 抗小鼠CD49b抗体，clone DX5
    抗小鼠CD49b抗体，clone DX5

    <p>抗小鼠CD49b（整合素α2）的大鼠Monoclonal IgM抗体</p>

质量声明:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.

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