EasySep™人记忆CD4+ T细胞富集试剂盒

免疫磁珠负选无磁珠标记的人记忆CD4+ T细胞

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产品号 #（选择产品）

产品号 #19157_C

免疫磁珠负选无磁珠标记的人记忆 CD4+ T细胞

产品优势

操作简单、快捷，且无需分离柱
纯度高达98%
获得的活细胞无磁珠标记

产品组分包括

EasySep™ 人记忆CD4+ T细胞富集试剂盒（产品号 #19157）

EasySep™人记忆CD4+ T细胞富集抗体混合物，1 mL
EasySep™ D Magnetic Particles磁珠，2 x 1 mL

RoboSep™ 人记忆CD4+ T细胞富集试剂盒（含滤芯吸头）（产品号 #19157RF）

EasySep™人记忆CD4+ T细胞富集抗体混合物，1 mL
EasySep™ D Magnetic Particles磁珠，2 x 1 mL
RoboSep™ 缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）

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总览

使用EasySep™人记忆CD4+ T细胞富集试剂盒，通过免疫磁珠负选，可轻松高效地从新鲜或冻存的人外周血单个核细胞（PBMCs）样本中分离高纯度的人记忆 CD4+ T细胞（CD4+CD45RA-CD45RO+）。EasySep™结合了单克隆抗体的特异性和无柱磁珠分选系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此 EasySep™负选过程中，表达以下标记物的非目的细胞通过抗体复合物与磁珠标记被靶向去除：CD8、CD14、CD16、CD19、CD20、CD36、CD45RA、CD56、CD123、TCRγ/δ及glyA。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需简单地将目的细胞倾倒或吸取至一个新的试管中即可。经磁珠分选获得的记忆CD4+ T细胞可直接用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择经EasySep™人记忆CD4+ T细胞富集试剂盒分离的、符合伦理规范的即用型冻存人外周血CD4+CD45RO+ T细胞。探索更多优化您实验流程的产品，包括培养基、添加物、抗体等。

常见问题 (11)

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (6)

Intermediate DNA methylation is a conserved signature of genome regulation Elliott G et al. Nature Communications 2015 DEC

Abstract

The role of intermediate methylation states in DNA is unclear. Here,to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity,we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers,exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation,are predominantly allele-independent and are conserved across individuals and between mouse and human,suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons,highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.

View publication

High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry. B. Fregin et al. Nature communications 2019

Abstract

In life sciences,the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently,cell rheological measurements were either limited by acquisition throughput,excessive post processing,or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes,but has been restricted to single material parameters as the Young's modulus. Here,we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition,our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.

View publication

PD-1 blockade potentiates HIV latency reversal ex vivo in CD4+ T cells from ART-suppressed individuals. R. Fromentin et al. Nature communications 2019 feb

Abstract

HIV persists in latently infected CD4+ T cells during antiretroviral therapy (ART). Immune checkpoint molecules,including PD-1,are preferentially expressed at the surface of persistently infected cells. However,whether PD-1 plays a functional role in HIV latency and reservoir persistence remains unknown. Using CD4+ T cells from HIV-infected individuals,we show that the engagement of PD-1 inhibits viral production at the transcriptional level and abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells. Conversely,PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production in combination with the latency reversing agent bryostatin without increasing T cell activation. Our results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency disruption.

View publication

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更多信息

更多信息
物种	人
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • Easy 50 EasySep™ Magnet (Catalog #18002) • RoboSep™-S (Catalog #21000)
样本来源	PBMC
Selection Method	Negative

EasySep™人记忆CD4+ T细胞富集试剂盒

产品号 #19157

产品号 #19157RF

产品号 #（选择产品）

产品号 #19157_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

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Scientists Helping Scientists™

EasySep™人记忆CD4+ T细胞富集试剂盒

产品号 #19157

产品号 #19157RF

产品号 #（选择产品）

产品号 #19157_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (10)

常见问题 (11)

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

文献 (6)

Abstract

Abstract

Abstract

Request Pricing

更多信息

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