EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒

对来源于外周血单个核细胞进行免疫磁珠负选分离，获取无磁珠标记的CD4⁺CD127lowCD49d⁻ T细胞

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¥15,290.00

产品号 #（选择产品）

产品号 #19232_C

免疫磁珠负选试剂盒

产品优势

操作简单、快捷，且无需分离柱
纯度高达87.4%
分选得到的细胞无磁珠标记

产品组分包括

EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒（产品号 #19232）

EasySep™人CD4+ CD127low CD49d-调节性T细胞富集抗体混合物，1 mL
EasySep™ 葡聚糖分选抗体混合物，1 mL
EasySep™ Magnetic Particles磁珠，3 x 1 mL

RoboSep™ CD4+CD127lowCD49d- 调节性T细胞富集试剂盒（产品号 #19232RF）

EasySep™人CD4+ CD127low CD49d- 调节性T细胞富集抗体混合物，1 mL
EasySep™葡聚糖分选抗体混合物，1 mL
EasySep™ Magnetic Particles磁珠，3 x 1 mL
RoboSep™缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）

立即询价

总览

使用 EasySep™ 人 CD4⁺CD127lowCD49d⁻ 调节性 T 细胞富集试剂盒，可通过免疫磁珠负选法从新鲜人外周血单个核细胞（PBMCs）中轻松高效地分离高纯度的 CD4⁺CD127lowCD49d⁻ T 细胞。EasySep™ 技术在已发表的研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁珠分选系统的简便性。

在此 EasySep™ 负选步骤中，非目的细胞被抗体复合物和磁珠标记。经 EasySep™ 磁极分离后，带有磁珠标记的非目的细胞与未标记的CD4⁺CD127lowCD49d⁻ T目的细胞分离。目的细胞可通过倾倒或移液方式转移到新试管中。分选完成后，分离得到的 CD4⁺CD127lowCD49d⁻ T 细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁珠技术的工作原理，或了解如何使用 RoboSep™ 实现全自动化免疫磁珠细胞分选。探索更多产品，包括培养基、添加物、抗体等，以优化您的实验流程。

实验数据

Figure 1. Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (1)

Abstract

Manipulation of the CD28/CTLA-4 pathway is at the heart of a number of immunomodulatory approaches used in both autoimmunity and cancer. Although it is clear that CTLA-4 is a critical regulator of T cell responses,the immunological contexts in which CTLA-4 controls immune responses are not well defined. In this study,we show that whereas CD80/CD86-dependent activation of resting human T cells caused extensive T cell proliferation and robust CTLA-4 expression,in this context CTLA-4 blocking Abs had no impact on the response. In contrast,in settings where CTLA-4(+) cells were present as regulators�

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更多信息

更多信息
物种	人
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源	PBMC
Selection Method	Negative

EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒

产品号 #19232

产品号 #19232RF

产品号 #（选择产品）

产品号 #19232_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

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Scientists Helping Scientists™

EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒

产品号 #19232

产品号 #19232RF

产品号 #（选择产品）

产品号 #19232_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (9)

常见问题

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

文献 (1)

Abstract

Request Pricing

更多信息

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