使用 EasySep™ HLA 全血 T 细胞富集试剂盒，可通过免疫磁性负选法从经 HetaSep™ 处理的全血或洗涤后的白细胞单采样本中轻松高效地分离高纯度的人 T 细胞。EasySep™ 技术在已发表研究中被广泛使用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。
在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物和磁性颗粒进行标记。表达以下标志物的细胞将被去除：CD14、CD16、CD19、CD20、CD33、CD36、CD42b、CD56、CD66b 和 CD123。经 EasySep™ 磁体分离后，带有磁性标记的不需要细胞被去除，未标记的目标 T 细胞通过倾倒或移液方式转移至新试管中。磁性细胞分离完成后，目标 T 细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。
请注意：根据欧洲议会及理事会于 2022 年生效的《体外诊断医疗器械法规 (EU Regulation 2017/746, IVDR)》，STEMCELL 已取消本产品的 CE IVD 认证声明，产品现标注为“仅供科研使用（For Research Use Only）”。产品的形式、功能及质量保证未发生任何变化。如需更多信息，请联系质量保证与法规部门（qaschangenote@stemcell.com）。EasySep™ Direct HLA B 细胞分离试剂盒（产品号#89684）在加拿大、欧盟、英国和澳大利亚销售。在其他地区，可使用等效产品 EasySep™ Direct HLA 交叉配型 B 细胞分离试剂盒（产品号#19684）。
Figure 1. FACS Histogram Results with EasySep™ HLA Whole Blood T Cell Enrichment Kit
Starting with HetaSep™-treated whole blood, the CD3+ cell content of the enriched fraction typically ranges from 93.1 - 98.0% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.1 - 3.1 x 10e6 CD3+ cells are typically recovered.
Can EasySep™ be used for either positive or negative selection?
Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
How does the separation work?
Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
Which columns do I use?
The EasySep™ procedure is column-free. That's right - no columns!
How can I analyze the purity of my enriched sample?
The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
Can EasySep™ separations be automated?
Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
Can EasySep™ be used to isolate rare cells?
Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.
Are the EasySep™ magnetic particles FACS-compatible?
Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
Can the EasySep™ magnetic particles be removed after enrichment?
No, but due to the small size of these particles, they will not interfere with downstream applications.
Can I alter the separation time in the magnet?
Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
For positive selection, can I perform more than 3 separations to increase purity?
Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
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EasySep™小鼠TIL（CD45）正选试剂盒
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