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EasySep™ HLA B细胞富集试剂盒

对来源于外周血单个核细胞或白细胞分离样本进行免疫磁珠负选分离，获得未标记的 B 细胞

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产品号 #（选择产品）

产品号 #19054HLA_C

免疫磁珠负选细胞分离试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达99%
  • 分选得到的细胞不带标记
  • 与下游HLA检测兼容

产品组分包括

  • EasySep™ HLA B细胞富集试剂盒（产品号 #19054HLA）
    • EasySep™HLA B细胞富集抗体混合物, 1 mL
    • EasySep™ D Magnetic Particles磁珠, 2 x 1 mL
  • RoboSep™ HLA B细胞富集试剂盒（带过滤吸头）（产品     号#19054HLARF）
    • EasySep™HLA B细胞富集抗体混合物, 1 mL
    • EasySep™ D Magnetic Particles磁珠, 2 x 1 mL    
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用 EasySep™ HLA B 细胞富集试剂盒，可通过免疫磁珠负选法从新鲜或冻存的人外周血单个核细胞（PBMCs）或白细胞单采样本中高效分离高纯度的人 B 细胞。EasySep™ 技术在发表研究中已被广泛使用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。

在此 EasySep™ 负选步骤中，不需要的细胞通过抗体复合物和磁性颗粒进行标记。经 EasySep™ 磁极分离后，未标记的目的 B 细胞被倒入新试管中。磁性分离完成后，B 细胞可直接用于流式细胞术、细胞培养或 DNA/RNA 提取等下游应用。

了解更多关于 EasySep™ 免疫磁性细胞分选技术的工作原理，或了解如何使用 RoboSep™ 实现免疫磁性细胞分离的全自动化。探索更多的产品，包括培养基、补充物、抗体等，以优化您的实验流程。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
B 细胞
 
种属
人
 
样本来源
PBMC
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
嵌合体，HLA，免疫
 

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实验数据

FACS Histogram Results with EasySep™ HLA B Cell Enrichment Kit

Figure 1. FACS Histogram Results with EasySep™ HLA B Cell Enrichment Kit

Starting with frozen mononuclear cells, the CD19+ cell content of the enriched fraction typically ranges from 95 - 99%.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA B Cell Enrichment Kit with Filter Tips
Catalog #
19054HLARF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ HLA B Cell Enrichment Kit
Catalog #
19054HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA B Cell Enrichment Kit with Filter Tips
Catalog #
19054HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA B Cell Enrichment Kit with Filter Tips
Catalog #
19054HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA B Cell Enrichment Kit with Filter Tips
Catalog #
19054HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA B Cell Enrichment Kit
Catalog #
19054HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA B Cell Enrichment Kit
Catalog #
19054HLA
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human B Cell Isolation Product Selection
Brochure
Human B Cell Isolation Product Selection
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (1)

Modified flow cytometry crossmatch detecting alloantibody-related cytotoxicity as a way to distinguish lytic antibodies from harmless in allosensitised kidney recipients. Zieliʼn et al. Transplantation proceedings 2013

Abstract

The serological complement-dependent cytotoxicity crossmatch (CDC-XM) permits routine identification of anti-donor alloantibodies in the sera of allotransplant recipients. However, in a small group of recipients, antibodies below the threshold of detection may still be responsible for hyperacute rejection. For the same reason, approximately 20% of recipients develop acute rejection episodes. The flow cytometry crossmatch (FCXM) was designed to address these problems, but because of the presence of clinically insignificant antibodies (linked, non-lytic), the FCXM appears to be too sensitive yielding false-positive results. We compared FCXM with its modified version assessing cell viability (cytolytic flow cytometry crossmatch; cFCXM) using sera from previously sensitised kidney recipients. The presence of alloantibodies was detected using the Luminex platform. The cFCXM proved to be of greater sensitivity than CDC-XM, which was additionally confirmed with bead-based Luminex techniques. The cFCXM was also superior to FCXM because it distinguished lytic from non-lytic antibodies. The cFCXM was superior to assess donor specificity, sensitivity, and detection of clinically relevant lytic antibodies.
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更多信息

更多信息
物种 人类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源 PBMC
Selection Method Negative
标记抗体
  1. 抗人CD20抗体，clone 2H7
    抗人CD20抗体，clone 2H7

    小鼠Monoclonal IgG2b抗体，抗人、恒河猴、食蟹猴CD20

  2. 抗人CD19抗体，clone HIB19
    抗人CD19抗体，clone HIB19

    抗人、黑猩猩CD19的小鼠Monoclonal IgG1抗体

质量保证:

本产品仅供科研使用，除非另有说明，不得用于人体或动物的诊断或治疗用途。如需了解 STEMCELL 的质量体系，请访问 www.stemcell.cn/compliance

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