EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72892_C
视黄醇通路激活剂；激活视黄酸受体（RAR）
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总览
TTNPB是视黄酸类似物，能强效且选择性地激活视黄酸受体(RAR），对RARα、RARβ和RARγ的EC₅₀分别为21、4和2.4 nM（Beard et al.; Wong et al）。
分化
·与CHIR99021或Activin A一起使用时，分别诱导人或小鼠多能干细胞分化为中间中胚层(Araoka et al.; Oeda et al.)。
·促进小鸡尾部神经板外植体中神经元的分化（Diez del Corral et al.）。
重编程
·可与CHIR99021、Tranylcypromine、丙戊酸、3-Deazaneplanocin A和E-616452一起使用，在无转基因因子的情况下，将小鼠胚胎成纤维细胞重编程为诱导多能干细胞（iPS）（Hou et al.）。
癌症研究
·诱导从骨髓增生异常综合征（MDS）患者分选的髓系祖细胞在体外生长并向粒细胞分化（Fabian et al.）。
细胞类型
癌细胞及细胞系，中胚层，PSC衍生，神经干/祖细胞，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
分化，重编程
研究领域
癌症，干细胞生物学
CAS 编号
71441-28-6
化学式
C₂₄H₂₈O₂
纯度
≥98%
通路
视黄醇类
靶点
RAR
产品说明书及文档
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相关材料与文献
技术资料 (3)
文献 (10)
Identification of a chemical tool for the orphan nuclear receptor FXR.
Journal of medicinal chemistry 2000 AUG
Abstract
Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension.
Neuron 2003 SEP
Abstract
Vertebrate body axis extension involves progressive generation and subsequent differentiation of new cells derived from a caudal stem zone; however,molecular mechanisms that preserve caudal progenitors and coordinate differentiation are poorly understood. FGF maintains caudal progenitors and its attenuation is required for neuronal and mesodermal differentiation and to position segment boundaries. Furthermore,somitic mesoderm promotes neuronal differentiation in part by downregulating Fgf8. Here we identify retinoic acid (RA) as this somitic signal and show that retinoid and FGF pathways have opposing actions. FGF is a general repressor of differentiation,including ventral neural patterning,while RA attenuates Fgf8 in neuroepithelium and paraxial mesoderm,where it controls somite boundary position. RA is further required for neuronal differentiation and expression of key ventral neural patterning genes. Our data demonstrate that FGF and RA pathways are mutually inhibitory and suggest that their opposing actions provide a global mechanism that controls differentiation during axis extension.
Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells.
Differentiation 2009 NOV
Abstract
Differentiation of embryonic and adult myogenic progenitors undergoes a complex series of cell rearrangements and specification events which are controlled by distinct gene regulatory networks. Delineation of the molecular mechanisms that regulate skeletal muscle specification and formation should be important for understanding congenital myopathies and muscular degenerative diseases. Retinoic acid (RA) signaling plays an important role in development. However,the role of RA signaling in adult myogenic progenitors is poorly understood. Here,we investigate the role of RA signaling in regulating myogenic differentiation of myoblastic progenitor cells. Using the mouse myoblast progenitor C2C12 line as a model,we have found that the endogenous expression of most RAR and RXR isotypes is readily detected. While the nuclear receptor co-repressors are highly expressed,two of the three nuclear receptor co-activators and the enzymes involved in RA synthesis are expressed at low level or undetectable,suggesting that the RA signaling pathway may be repressed in myogenic progenitors. Using the alpha-myosin heavy chain promoter-driven reporter (MyHC-GLuc),we have demonstrated that either ATRA or 9CRA is able to effectively induce myogenic differentiation,which can be synergistically enhanced when both ATRA and 9CRA are used. Upon ATRA and 9CRA treatment of C2C12 cells the expression of late myogenic markers significantly increases. We have further shown that adenovirus-mediated exogenous expression of RARalpha and/or RXRalpha is able to effectively induce myogenic differentiation in a ligand-independent fashion. Morphologically,ATRA- and 9CRA-treated C2C12 cells exhibit elongated cell body and become multi-nucleated myoblasts,and even form myoblast fusion. Ultrastructural analysis under transmission electron microscope reveals that RA-treated myogenic progenitor cells exhibit an abundant presence of muscle fibers. Therefore,our results strongly suggest that RA signaling may play an important role in regulating myogenic differentiation.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 71441-28-6 |
| Chemical Formula | C₂₄H₂₈O₂ |
| 纯度 | ≥ 98% |
| Target | RAR |
| Pathway | Retinoid |
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