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STEMdiff™ 心肌细胞铺板补充剂

人多能干细胞来源的心肌细胞在冻融及收获后提高铺板效率的专用补充剂

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产品号 #100-1120_C

一种用于提高人多能干细胞衍生心肌细胞在复苏和收获后的铺板效率的专用培养试剂盒

产品优势

  • Improve plating efficiency and double the number of hPSC-derived cardiomyocytes available for your experiments
  • Retain the stable electrical profile of hPSC-derived cardiomyocytes following replating
  • Save time and money with more efficient cardiomyocyte replating after thawing and dissociation
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总览
实验数据
产品说明书及文档
应用领域

总览

使用STEMdiff™心肌细胞接种补充剂，可使人多能干细胞（hPSC）来源的心肌细胞在解离、复苏和重新接种后产量提高两倍。与STEMdiff™心肌细胞支持培养基配合使用时，STEMdiff™ 心肌细胞接种补充剂可提高接种效率并最大限度地减少 hPSC 来源心肌细胞的损失。

本产品专为使用 STEMdiff™ 心室肌细胞分化试剂盒或 STEMdiff™ 心房肌细胞分化试剂盒生成的 hPSC 来源心肌细胞而设计。通过提高接种效率，STEMdiff™心肌细胞接补充剂可增加实验通量。

请将本产品作为完整、优化的工作流程的一部分使用，该流程应包含以下兼容的 STEMdiff™ 心肌细胞产品： 

STEMdiff™心室肌细胞分化试剂盒
STEMdiff™心房肌细胞分化试剂盒
STEMdiff™心肌细胞解离试剂盒
STEMdiff™心肌细胞支持培养基
STEMdiff™心肌细胞冻存培养基
STEMdiff™心肌细胞维持试剂盒

 

包含
血清
 
细胞类型
心肌细胞，PSC衍生
 
种属
人
 
应用
冻存，分化，培养
 
品牌
STEMdiff
 
研究领域
疾病建模，药物发现和毒理检测，干细胞生物学，细胞治疗开发
 

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Data Figures

Graphs demonstrating iImproved plating: STEMdiff™ Cardiomyocyte Plating Medium (51%) vs. Support Medium (16%).

Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).

Microscope images showing enhanced attachment of replated hPSC-derived cardiomyocytes using plating medium.

Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.

Microelectrode array shows stable beat rate of cardiomyocytes replated with this kit.

Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile

Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X)
Catalog #
100-1120
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X)
Catalog #
100-1120
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X)
Catalog #
100-1120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Contains Serum
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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