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RosetteSep™人脐带血祖细胞完全富集试剂盒

免疫密度负选试剂
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产品号 #（选择产品）

产品号 #15276_C

包含RosetteSep™人脐带血祖细胞试剂盒和HetaSep™

产品优势

  •  易于操作、快速
  • 无需特殊设备或培训
  • 获得的活细胞无标记

产品组分包括

  • RosetteSep™人脐带血祖细胞富集试剂盒（产品号#15276）
  • RosetteSep™人造血祖细胞富集抗体混合物，2 mL (产品号#15026) x 2
  • HetaSep™，100 mL（产品号#07906）
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. Lymphoprep™
    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™人脐带血祖细胞富集混合物（含HetaSep™）专为通过负选法从脐带血中分离祖细胞而设计。HetaSep™（产品号#07906）可通过沉降浓缩样本，从而减少所需RosetteSep™试剂量。经HetaSep™浓缩后，使用改进版RosetteSep™方案即可比未浓缩样本使用更少试剂。非目标细胞通过识别CD2、CD3、CD14、CD16、CD19、CD24、CD56、CD61、CD66b及红细胞上糖蛋白A的四聚体抗体复合物被靶向去除。当在密度梯度介质上离心时，非目标细胞与红细胞一起沉淀。纯化后的祖细胞作为高富集群体出现在血浆与密度介质的交界面处。

由于供应变更，本产品不再附带Ficoll-Paque™ Plus。建议使用Lymphoprep™（产品号#18060）进行密度梯度分离。Lymphoprep™密度为1.077 g/mL，与Ficoll-Paque™相同，离心后单个核细胞停留在血浆与Lymphoprep™界面，而粒细胞与红细胞沉降于底部。

 

分类
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
人
 
样本来源
脐带血
 
分选方法
负选
 
应用
细胞分选
 
品牌
RosetteSep
 
研究领域
免疫，干细胞生物学
 

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实验数据

FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor CellEnrichment Kit

Figure 1. FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor Cell Enrichment Kit

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 29 ± 9%.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit
Catalog #
15276
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (6)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™

常见问题

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
物种 人类
样本来源 脐带血
Selection Method Negative
标记抗体
  1. 抗人CD34抗体，clone 581
    抗人CD34抗体，clone 581

    小鼠Monoclonal IgG1抗体，抗人CD34

  2. 抗人CD34抗体，clone 8G12
    抗人CD34抗体，clone 8G12

    小鼠Monoclonal IgG1抗体，抗人CD34

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产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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