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含抗CD56的RosetteSep™ CTC富集抗体混合物

免疫密度负选试剂混合物
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产品号 #（选择产品）

产品号 #15137_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 获得的活细胞无标记
  • 可与SepMate™联合使用，实现一致的高通量样本处理

产品组分包括

  • 含抗CD56的 RosetteSep™ CTC富集抗体混合物（产品号#15137）
    • 含抗CD56的RosetteSep™ CTC富集抗体混合物，2mL
  • 含抗CD56的 RosetteSep™ CTC富集抗体混合物（产品号#15177）
    • 含抗CD56的 RosetteSep™ CTC富集抗体混合物，5x2mL
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  1. Lymphoprep™
    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

RosetteSep™ CTC富集混合物（含抗CD56）专为通过负选法从新鲜全血中富集循环上皮肿瘤细胞而设计。非目标细胞通过识别CD3、CD14、CD16、CD19、CD38、CD45、CD56、CD61、CD66b和糖蛋白A的四聚体抗体复合物被靶向去除。当在Lymphoprep™（产品号#18060）等密度梯度介质上离心时，非目标细胞与红细胞一起沉淀。纯化后的肿瘤细胞作为高富集群体出现在血浆与密度介质的交界面处。

本混合物适用于乳腺癌样本；若研究小细胞癌样本（包括肺癌），请使用RosetteSep™ CTC富集混合物（含抗CD36）（产品号#15127）。

 

分类
细胞分选试剂盒
 
细胞类型
癌细胞及细胞系
 
种属
人
 
样本来源
白膜层、全血
 
分选方法
负选
 
应用
细胞分选
 
品牌
RosetteSep
 
研究领域
癌症，免疫
 

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实验数据

Typical RosetteSep™ CTC Enrichment Profile

Figure 1. Typical RosetteSep™ CTC Enrichment Profile

In the example above, CAMA (epithelial tumor cell line) cells were seeded into whole blood at a starting frequency of 0.8%. The CAMA cell content of the enriched fraction is 60%. Typically 3.2 to 4.4 log depletion of targeted CD45+ cells is attained.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56
Catalog #
15177, 15137
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56
Catalog #
15177, 15137
Lot #
All
Language
English

相关材料与文献

技术资料 (4)

Isolation of Circulating Tumor Cells
Brochure
Isolation of Circulating Tumor Cells
Tools for Cancer Research
Brochure
Tools for Cancer Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...

常见问题

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

文献 (3)

How to study and overcome tumor heterogeneity with circulating biomarkers: The breast cancer case. V. Appierto et al. Seminars in cancer biology 2017 APR

Abstract

Breast cancer ranks first among female cancer-related deaths in Western countries. As the primary tumor can often be controlled by surgical resection, the survival of women with breast cancer is closely linked to the incidence of distant metastases. Molecular screening by next generation sequencing highlighted the spatial and temporal heterogeneity of solid tumors as well as the clonal evolution of cancer cells during progression and under treatment pressure. Such findings question whether an optimal assessment of disease progression and a screening for druggable mutations should be based on molecular features of primary or recurrent/metastatic lesions and therefore represent a crucial element for failure or success of personalized medicine. In fact, new targeted therapies may induce only short-term benefit annulled by the emergence of resistant clones with new driver mutations which would need to be rapidly and reliably identified. Serial tissue sampling is therefore essential but, unfortunately, also represents a problem since biopsies from solid lesions, which are invasive and potentially painful and risky, cannot be easily repeatedly sampled, are inaccessible or may not fully reflect tumor heterogeneity. The need to early detect and strike this moving target" is now directing the scientific community towards liquid biopsy-based biomarkers which include circulating tumor cells (CTC) and cell-free circulating tumor DNA (ctDNA) can be repeatedly assessed through non-invasive and easy-to-perform procedures and may act as reliable read-outs of functional and molecular features of recurrent/metastatic lesions. In this review we summarize the outcome of CTCs and ctDNA in breast cancer with special reference on their role on unveiling and overcoming tumor heterogeneity on their potential relevance for tumor surveillance and monitoring and for the selection of therapeutic options. Finally we propose integration between blood-based molecular and clinical approaches for monitoring disease progression according to the specific pattern of recurrence of the most aggressive breast cancer molecular subtypes."
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Improved detection of circulating tumor cells in non-metastatic high-risk prostate cancer patients. A. Kuske et al. Scientific reports 2016 DEC

Abstract

The relevance of blood-based assays to monitor minimal residual disease (MRD) in non-metastatic prostate cancer (PCa) remains unclear. Proving that clinically relevant circulating tumor cells (CTCs) can be detected with available technologies could address this. This study aimed to improve CTC detection in non-metastatic PCa patients by combining three independent CTC assays: the CellSearch system, an in vivo CellCollector and the EPISPOT. Peripheral blood samples from high-risk PCa patients were screened for CTCs before and three months after radical prostatectomy (RP). Combining the results of both time points, CTCs were detected in 37{\%}, 54.9{\%} and 58.7{\%} of patients using CellSearch, CellCollector and EPISPOT, respectively. The cumulative positivity rate of the three CTC assays was 81.3{\%} (87/107) with 21.5{\%} (23/107) of patients harboring ≥5 CTCs/7.5 ml blood. Matched pair analysis of 30 blood samples taken before and after surgery indicated a significant decrease in CTCs captured by the CellCollector from 66{\%} before RP to 34{\%} after therapy (p = 0.031). CTC detection by EPISPOT before RP significantly correlated with PSA serum values (p {\textless} 0.0001) and clinical tumor stage (p = 0.04), while the other assays showed no significant correlations. In conclusion, CTC-based liquid biopsies have the potential to monitor MRD in patients with non-metastatic prostate cancer.
View publication
Tumorigenicity and genetic profiling of circulating tumor cells in small-cell lung cancer. C. L. Hodgkinson et al. Nature medicine 2014 AUG

Abstract

Small-cell lung cancer (SCLC), an aggressive neuroendocrine tumor with early dissemination and dismal prognosis, accounts for 15-20{\%} of lung cancer cases and ∼200,000 deaths each year. Most cases are inoperable, and biopsies to investigate SCLC biology are rarely obtainable. Circulating tumor cells (CTCs), which are prevalent in SCLC, present a readily accessible 'liquid biopsy'. Here we show that CTCs from patients with either chemosensitive or chemorefractory SCLC are tumorigenic in immune-compromised mice, and the resultant CTC-derived explants (CDXs) mirror the donor patient's response to platinum and etoposide chemotherapy. Genomic analysis of isolated CTCs revealed considerable similarity to the corresponding CDX. Most marked differences were observed between CDXs from patients with different clinical outcomes. These data demonstrate that CTC molecular analysis via serial blood sampling could facilitate delivery of personalized medicine for SCLC. CDXs are readily passaged, and these unique mouse models provide tractable systems for therapy testing and understanding drug resistance mechanisms.
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更多信息

更多信息
物种 人类
样本来源 全血, 白膜层
Selection Method Negative
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