EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-1120_C
一种用于提高人多能干细胞衍生心肌细胞在复苏和收集后的铺板效率的专用培养试剂盒
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总览
使用STEMdiff™心肌细胞接种添加物，可使人多能干细胞（hPSC）来源的心肌细胞在解离、复苏和重新接种后得率提高两倍。与STEMdiff™心肌细胞支持培养基配合使用时，STEMdiff™ 心肌细胞接种补充剂可提高接种效率并最大限度地减少 hPSC 来源心肌细胞的损失。
本产品专为使用 STEMdiff™ 心室肌细胞分化试剂盒或 STEMdiff™ 心房肌细胞分化试剂盒生成的 hPSC 来源心肌细胞而设计。通过提高接种效率，STEMdiff™心肌细胞接补充剂可增加实验通量。
请将本产品作为完整、优化的工作流程的一部分使用，该流程应包含以下兼容的 STEMdiff™ 心肌细胞产品：
STEMdiff™心室肌细胞分化试剂盒
STEMdiff™心房肌细胞分化试剂盒
STEMdiff™心肌细胞解离试剂盒
STEMdiff™心肌细胞支持培养基
STEMdiff™心肌细胞冻存培养基
STEMdiff™心肌细胞维持试剂盒
包含
血清
细胞类型
心肌细胞，PSC衍生
种属
人
应用
冻存，分化，培养
品牌
STEMdiff
研究领域
疾病建模，药物发现和毒理检测，干细胞生物学，细胞治疗开发
实验数据
Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit
Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).
Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit
Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.
Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile
Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1 Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X) Catalog #
100-1120 Lot #
All Language
English Document Type
Product Information Sheet 2 Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X) Catalog #
100-1120 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
STEMdiff™ Cardiomyocyte Plating Supplement (100X) Catalog #
100-1120 Lot #
All Language
English 应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
Research Area
Workflow Stages
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Cardiac and Skeletal Muscle Research
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更多信息
| 物种 | 人类 |
|---|---|
| Contains | Serum |
法律声明：
本产品仅供研究使用，除非另有说明，不得用于人类或动物的诊断或治疗用途。如需了解STEMCELL质量的更多信息，请参阅 WWW.STEMCELL.CN/COMPLIANCE。
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