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EasySep™ Release 小鼠APC正选试剂盒

采用磁珠解离技术，对小鼠组织来源细胞样本中标记 APC 偶联抗体的细胞进行免疫磁珠正选分离，分离后的细胞上无磁珠

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产品号 #（选择产品）

产品号 #100-0033_C

采用可解离磁珠技术的免疫磁珠正选试剂盒

产品优势

  • 在40分钟内从小鼠组织中分选出用APC偶联抗体标记的高纯度细胞
  • 无需清洗去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

  • EasySep™ Release 小鼠APC正选试剂盒（产品号 #100-0033）
    • EasySep™ Release APC正选抗体混合物，1 mL
    • EasySep™ Releasable RapidSpheres™磁珠，1 mL
    • EasySep™Release 缓冲液（浓缩），3 x 1 mL
    • 小鼠FcR阻断剂（产品号 #18731），5 mL
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

使用EasySep™可解离APC正选试剂盒，可从小鼠脾脏细胞等样本中通过免疫磁性正选法分离高纯度藻蓝蛋白（APC）偶联抗体标记的细胞。EasySep™技术在已发表的研究中被广泛应用超过20年，将单克隆抗体的特异性与无柱磁分离系统的简便性相结合。


该EasySep™正选步骤包括使用可识别APC的抗体复合物和EasySep™可解离RapidSpheres™对目标细胞进行标记。与传统磁性颗粒始终结合在目标细胞上的方式不同，该试剂盒中的RapidSpheres™具有可解离特性。目标细胞首先被抗体和这些特殊的磁性颗粒标记，然后使用EasySep™磁极在无柱的情况下进行分离。非目标细胞被简单地倒出，而目标细胞保留在试管中。随后，使用解离剂从EasySep™分离的APC偶联抗体标记细胞中去除结合在细胞表面的磁性颗粒。

使用此EasySep™解离试剂盒进行磁性细胞分离后，目标细胞可立即用于下游应用，如流式细胞术、细胞培养或DNA/RNA提取。抗体复合物仍结合在目标细胞表面，可能与Brilliant Violet™抗体偶联物、聚乙二醇修饰蛋白或其他化学相关配体发生相互作用。了解更多关于免疫磁性EasySep™技术的工作原理。探索更多产品，包括培养基、添加剂、抗体等，为您的实验流程提供优化方案。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyPlate™ EasySep™磁极（产品号 #18102）
• EasyEights™ EasySep™磁极（产品号 #18103）
 
分类
细胞分选试剂盒
 
细胞类型
其他物种
 
种属
小鼠
 
样本来源
骨髓，其他物种，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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实验数据

Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
1000157896 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
1000157897 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English

相关材料与文献

技术资料 (4)

Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres�...
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Ce...
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable RapidSpheres™ Results in Functional, Pure and Particle-Free Cells
Scientific Poster
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells w...

文献 (1)

Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging. A. K. Jaiswal et al. American journal of physiology. Lung cellular and molecular physiology 2022 jan

Abstract

Asthma and its heterogeneity change with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adults with asthma. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To study the changes in inflammatory mechanism, we used a clinically relevant treatment model of house-dust mite (HDM)-induced allergic asthma and investigated lung adaptive immune response in adult (20-22 wk old) and aged (80-82 wk old) mice. Our result indicates an age-dependent increase in airway hyperresponsiveness (AHR), mixed granulomatous airway inflammation comprising eosinophils and neutrophils, and Th1/Th17 immune response with progressive decrease in frequencies and numbers of HDM-bearing dendritic cells (DC) accumulation in the draining lymph node (DLn) of aged mice as compared with adult mice. RNA-Seq experiments of the aged lung revealed short palate, lung, and nasal epithelial clone 1 (SPLUNC1) as one of the steroid-responsive genes, which progressively declined with age and further by HDM-induced inflammation. Moreover, we found increased glycolytic reprogramming, maturation/activation of DCs, the proliferation of OT-II cells, and Th2 cytokine secretion with recombinant SPLUNC1 (rSPLUNC1) treatment. Our results indicate a novel immunomodulatory role of SPLUNC1 regulating metabolic adaptation/maturation of DC. An age-dependent decline in the SPLUNC1 level may be involved in developing steroid-resistant airway inflammation and asthma heterogeneity.
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更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102)
样本来源 其它细胞系, 脾脏, 骨髓
Selection Method Positive
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