EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72182_C
MEK/ERK通路抑制剂；抑制MEK
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总览
PD0325901 是一种选择性、可穿透细胞的 MEK/ERK 通路抑制剂，可抑制 MEK 的激活及其下游信号传导。它是一种强效抑制剂，可在极低浓度（IC50 = 0.33 nM）下抑制 C26 细胞中 ERK 的磷酸化（Bain et al., Barrett et al.）。
维持和自我更新
·与 CHIR99021 联合使用，在没有 LIF 的情况下维持小鼠胚胎干细胞 (ES) 的分化 （Ying et al.）。
·可用于大鼠 ES 细胞的衍生和维持（Buehr et al., Li P et al.）。
重编程
·在重编程后期添加，以筛选和扩增完全重编程的小鼠诱导多能 (iPS) 细胞 （Shi et al., Silva et al.）。
·与 SB431542 和 Thiazovivin 联合使用，可提高人体细胞重编程为iPS细胞的效率（Lin et al.）。
·仅使用单一因子 OCT4 即可促进人体细胞重编程为iPS细胞（Zhu et al.）。
·与 CHIR99021 和 A83-01 联合使用，可从人和大鼠体细胞中生成 mouse-like 或“ground state” iPS细胞（Li W et al.）。
细胞类型
多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
培养，重编程
研究领域
干细胞生物学
CAS 编号
391210-10-9
化学式
C₁₆H₁₄F₃IN₂O₄
纯度
≥98%
通路
MEK/ERK
靶点
MEK
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (10)
The selectivity of protein kinase inhibitors: a further update.
The Biochemical journal 2007 DEC
Abstract
The specificities of 65 compounds reported to be relatively specific inhibitors of protein kinases have been profiled against a panel of 70-80 protein kinases. On the basis of this information,the effects of compounds that we have studied in cells and other data in the literature,we recommend the use of the following small-molecule inhibitors: SB 203580/SB202190 and BIRB 0796 to be used in parallel to assess the physiological roles of p38 MAPK (mitogen-activated protein kinase) isoforms,PI-103 and wortmannin to be used in parallel to inhibit phosphatidylinositol (phosphoinositide) 3-kinases,PP1 or PP2 to be used in parallel with Src-I1 (Src inhibitor-1) to inhibit Src family members; PD 184352 or PD 0325901 to inhibit MKK1 (MAPK kinase-1) or MKK1 plus MKK5,Akt-I-1/2 to inhibit the activation of PKB (protein kinase B/Akt),rapamycin to inhibit TORC1 [mTOR (mammalian target of rapamycin)-raptor (regulatory associated protein of mTOR) complex],CT 99021 to inhibit GSK3 (glycogen synthase kinase 3),BI-D1870 and SL0101 or FMK (fluoromethylketone) to be used in parallel to inhibit RSK (ribosomal S6 kinase),D4476 to inhibit CK1 (casein kinase 1),VX680 to inhibit Aurora kinases,and roscovitine as a pan-CDK (cyclin-dependent kinase) inhibitor. We have also identified harmine as a potent and specific inhibitor of DYRK1A (dual-specificity tyrosine-phosphorylated and -regulated kinase 1A) in vitro. The results have further emphasized the need for considerable caution in using small-molecule inhibitors of protein kinases to assess the physiological roles of these enzymes. Despite being used widely,many of the compounds that we analysed were too non-specific for useful conclusions to be made,other than to exclude the involvement of particular protein kinases in cellular processes.
The ground state of embryonic stem cell self-renewal.
Nature 2008 MAY
Abstract
In the three decades since pluripotent mouse embryonic stem (ES) cells were first described they have been derived and maintained by using various empirical combinations of feeder cells,conditioned media,cytokines,growth factors,hormones,fetal calf serum,and serum extracts. Consequently ES-cell self-renewal is generally considered to be dependent on multifactorial stimulation of dedicated transcriptional circuitries,pre-eminent among which is the activation of STAT3 by cytokines (ref. 8). Here we show,however,that extrinsic stimuli are dispensable for the derivation,propagation and pluripotency of ES cells. Self-renewal is enabled by the elimination of differentiation-inducing signalling from mitogen-activated protein kinase. Additional inhibition of glycogen synthase kinase 3 consolidates biosynthetic capacity and suppresses residual differentiation. Complete bypass of cytokine signalling is confirmed by isolating ES cells genetically devoid of STAT3. These findings reveal that ES cells have an innate programme for self-replication that does not require extrinsic instruction. This property may account for their latent tumorigenicity. The delineation of minimal requirements for self-renewal now provides a defined platform for the precise description and dissection of the pluripotent state.
A combined chemical and genetic approach for the generation of induced pluripotent stem cells.
Cell stem cell 2008 JUN
Abstract
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 391210-10-9 |
| Chemical Formula | C₁₆H₁₄F₃IN₂O₄ |
| 纯度 | ≥ 98% |
| Target | MEK |
| Pathway | MEK/ERK |
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