EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05445
用于人间充质干细胞的无动物成分培养基
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总览
使用MesenCult™-ACF Plus培养基实现人间充质基质细胞（MSCs）的一致、无血清扩增。该培养基不含动物成分（ACF) 和细胞外囊泡（EV），可支持来自多种组织来源（包括骨髓和脂肪组织）的MSC的高效增殖，同时保留MSC表型和三谱系分化潜能。
与含血清或去除EV的培养基相比，MesenCult™-ACF Plus提供高效的MSC扩增，并消除了血清来源成分和外源EV带来的变异性。干净、无EV的背景还支持下游EV研究，无需切换培养基。有关使用该系统生成EV的指导说明，请参阅我们详细的EV生成方案，并与EasySep™人细胞外囊泡正选试剂盒配合使用，以实现高效和标准化的EV回收。
MesenCult™-ACF Plus培养基试剂盒专为生成、扩增和冻存MSC以及将人多能干细胞分化为间充质祖细胞而设计，并经过优化，可实现高效一致的MSC培养。
对于无动物成分和优化的冻存，推荐使用MesenCult™-ACF冻存液来处理之前在MesenCult™培养基（包括MesenCult™-ACF Plus）中培养的人MSC。有关相关产品的完整列表（包括可用的分化培养基），请访问我们的MSC研究领域页面或通过info.cn@stemcell.com联系我们。
注意：MesenCult™-ACF Plus完全培养基必须添加L-谷氨酰胺。此外，它还需与不含动物成分的细胞附着基质配合使用，该基质作为MesenCult™-ACF Plus培养试剂盒的一部分提供。
CollPlant是细胞贴附基质中重组人胶原蛋白（rhCollagen）成分的制造商。
分类
专用培养基
细胞类型
间充质细胞，PSC衍生，间充质干/祖细胞
种属
人
应用
细胞培养，扩增，培养
品牌
MesenCult
研究领域
药物发现和毒性检测，细胞外囊泡研究，干细胞生物学
制剂类别
不含动物成分，无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (13)
文献 (16)
Characterization and Therapeutic Application of Mesenchymal Stem Cells with Neuromesodermal Origin from Human Pluripotent Stem Cells.
Theranostics 2019
Abstract
Rationale: Mesenchymal stem cells (MSC) hold great promise in the treatment of various diseases including autoimmune diseases,inflammatory diseases,etc.,due to their pleiotropic properties. However,largely incongruent data were obtained from different MSC-based clinical trials,which may be partially due to functional heterogeneity among MSC. Here,we attempt to derive homogeneous mesenchymal stem cells with neuromesodermal origin from human pluripotent stem cells (hPSC) and evaluate their functional properties. Methods: Growth factors and/or small molecules were used for the differentiation of human pluripotent stem cells (hPSC) into neuromesodermal progenitors (NMP),which were then cultured in animal component-free and serum-free induction medium for the derivation and long-term expansion of MSC. The resulted NMP-MSC were detailed characterized by analyzing their surface marker expression,proliferation,migration,multipotency,immunomodulatory activity and global gene expression profile. Moreover,the in vivo therapeutic potential of NMP-MSC was detected in a mouse model of contact hypersensitivity (CHS). Results: We demonstrate that NMP-MSC express posterior HOX genes and exhibit characteristics similar to those of bone marrow MSC (BMSC),and NMP-MSC derived from different hPSC lines show high level of similarity in global gene expression profiles. More importantly,NMP-MSC display much stronger immunomodulatory activity than BMSC in vitro and in vivo,as revealed by decreased inflammatory cell infiltration and diminished production of pro-inflammatory cytokines in inflamed tissue of CHS models. Conclusion: Our results identify NMP as a new source of MSC and suggest that functional and homogeneous NMP-MSC could serve as a candidate for MSC-based therapies.
Glycyrrhetinic Acid Maintains Intestinal Homeostasis via HuR.
Frontiers in pharmacology 2019
Abstract
Glycyrrhetinic acid (GA) is one of the main components of the traditional Chinese medicine of licorice,which can coordinate and promote the effects of other medicines in the traditional prescription. We found that GA could promote the proliferation,decrease the apoptotic rate,and attenuate DFMO-elicited growth arrest and delay in restitution after wounding in IEC-6 cells via HuR. GA failed to promote proliferation and to suppress apoptosis after silencing HuR by siRNA in IEC-6 cells. Furthermore,with the model of small intestinal organoids developed from intestinal crypt stem cells,we found that GA could increase HuR and its downstream ki67 levels to promote intestinal organoid development. In the in vivo assay,GA was shown to maintain the integrity of the intestinal epithelium under the circumstance of 48 h-fasting in rats via raising HuR and its downstream genes such as EGF,EGFR,and MEK. These results suggested that via HuR modulation,GA could promote intestinal epithelium homeostasis,and therefore contribute to the absorption of constituents from other medicines co-existing in the traditional prescription with licorice in the small intestine. Our results provide a new perspective for understanding the effect of licorice on enhancing the therapeutic effect of traditional prescriptions according to the traditional Chinese medicine theory.
Gestational Tissue-Derived Human Mesenchymal Stem Cells Use Distinct Combinations of Bioactive Molecules to Suppress the Proliferation of Human Hepatoblastoma and Colorectal Cancer Cells.
Stem cells international 2019
Abstract
Background Cancer has been considered a serious global health problem and a leading cause of morbidity and mortality worldwide. Despite recent advances in cancer therapy,treatments of advance stage cancers are mostly ineffective resulting in poor survival of patients. Recent evidences suggest that multipotent human mesenchymal stem cells (hMSCs) play important roles in growth and metastasis of several cancers by enhancing their engraftment and inducing tumor neovascularization. However,the effect of hMSCs on cancer cells is still controversial because there are also evidences demonstrating that hMSCs inhibited growth and metastasis of some cancers. Methods In this study,we investigated the effects of bioactive molecules released from bone marrow and gestational tissue-derived hMSCs on the proliferation of various human cancer cells,including C3A,HT29,A549,Saos-2,and U251. We also characterized the hMSC-derived factors that inhibit cancer cell proliferation by protein fractionation and mass spectrometry analysis. Results We herein make a direct comparison and show that the effects of hMSCs on cancer cell proliferation and migration depend on both hMSC sources and cancer cell types and cancer-derived bioactive molecules did not affect the cancer suppressive capacity of hMSCs. Moreover,hMSCs use distinct combination of bioactive molecules to suppress the proliferation of human hepatoblastoma and colorectal cancer cells. Using protein fractionation and mass spectrometry analysis,we have identified several novel hMSC-derived factors that might be able to suppress cancer cell proliferation. Conclusion We believe that the procedure developed in this study could be used to discover other therapeutically useful molecules released by various hMSC sources for a future in vivo study.
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 不含动物成分, 无血清 |
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