EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #70024_C
冻存的人原代细胞
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概览
选择即用型且来源符合伦理规范的原代人pan-T细胞。凭借定制化服务、定制产品、灵活的交付时间以及保留整个批次用于预筛细胞以进行下游应用，我们将帮助您高效获得所需的细胞。
pan-T通过使用免疫磁珠负选从外周血单个核细胞中分离获得，并在无动物成分的CryoStor®CS10 冻存液（产品号07930）中进行冻存，其由CD4和CD8 T细胞以及一些γ/δ T细胞亚群组成。该细胞使用机构审查委员会（IRB）批准的同意书和方案获取，并可按照要求提供其他文档和高分辨率HLA分型（I类和II类等位基因以及CMV状态）。收集过程中加入了酸性柠檬酸葡萄糖溶液A（ACDA）作为抗凝剂。选择产品选项后，可以申请查看供体规格（例如BMI类别、吸烟状况、种族等）。供体接受了HIV-1、HIV-2、乙型肝炎和丙型肝炎的筛查。
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浏览我们关于原代细胞的常见问题解答（FAQs）。
包含
• CryoStor® CS10
分类
冻存
细胞类型
T 细胞
物种
人
细胞和组织来源
外周血
研究领域
药物发现和毒性检测
供体状态
正常
纯度
≥ 85% CD3+（通过流式分析检测）
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (8)
文献 (3)
Paediatric patients with acute leukaemia and KMT2A (MLL) rearrangement show a distinctive expression pattern of histone deacetylases. British journal of haematology 2018 AUG
Abstract
Histone deacetylase inhibitors (HDACi) had emerged as promising drugs in leukaemia, but their toxicity due to lack of specificity limited their use. Therefore, there is a need to elucidate the role of HDACs in specific settings. The study of HDAC expression in childhood leukaemia could help to choose more specific HDACi for selected candidates in a personalized approach. We analysed HDAC1-11, SIRT1, SIRT7, MEF2C and MEF2D mRNA expression in 211 paediatric patients diagnosed with acute leukaemia. There was a global overexpression of HDACs, while specific HDACs correlated with clinical and biological features, and some even predicted outcome. Thus, some HDAC and MEF2C profiles probably reflected the lineage and the maturation of the blasts and some profiles identified specific oncogenic pathways active in the leukaemic cells. Specifically, we identified a distinctive signature for patients with KMT2A (MLL) rearrangement, with high HDAC9 and MEF2D expression, regardless of age, KMT2A partner and lineage. Moreover, we observed an adverse prognostic value of HDAC9 overexpression, regardless of KMT2A rearrangement. Our results provide useful knowledge on the complex picture of HDAC expression in childhood leukaemia and support the directed use of specific HDACi to selected paediatric patients with acute leukaemia.
Human dendritic cells derived from embryonic stem cells stably modified with CD1d efficiently stimulate antitumor invariant natural killer T cell response. Stem cells translational medicine 2014 JAN
Abstract
Invariant natural killer T (iNKT) cells are a unique lymphocyte subpopulation that mediates antitumor activities upon activation. A current strategy to harness iNKT cells for cancer treatment is endogenous iNKT cell activation using patient-derived dendritic cells (DCs). However, the limited number and functional defects of patient DCs are still the major challenges for this therapeutic approach. In this study, we investigated whether human embryonic stem cells (hESCs) with an ectopically expressed CD1d gene could be exploited to address this issue. Using a lentivector carrying an optimized expression cassette, we generated stably modified hESC lines that consistently overexpressed CD1d. These modified hESC lines were able to differentiate into DCs as efficiently as the parental line. Most importantly, more than 50% of such derived DCs were CD1d+. These CD1d-overexpressing DCs were more efficient in inducing iNKT cell response than those without modification, and their ability was comparable to that of DCs generated from monocytes of healthy donors. The iNKT cells expanded by the CD1d-overexpressing DCs were functional, as demonstrated by their ability to lyse iNKT cell-sensitive glioma cells. Therefore, hESCs stably modified with the CD1d gene may serve as a convenient, unlimited, and competent DC source for iNKT cell-based cancer immunotherapy.
Enhancing Immunostimulatory Function of Human Embryonic Stem Cell-Derived Dendritic Cells by CD1d Overexpression The Journal of Immunology 2012 MAY
Abstract
Human embryonic stem cell-derived dendritic cells (hESC-DCs) may potentially provide a platform to generate off-the-shelf" therapeutic cancer vaccines. To apply hESC-DCs for cancer immunotherapy in a semiallogeneic setting�
更多信息
| 物种 | 人 |
|---|---|
| Contains | CryoStor® CS10 |
| 纯度 | ≥ 85% CD3+ by flow cytometry |
| 细胞与组织来源 | 外周血 |
| 捐献者身份 | 正常 |
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