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人iPSC衍生的小胶质细胞

从人诱导多能干细胞系SCTi003-A分化而来的冻存小胶质细胞
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从人诱导多能干细胞系SCTi003-A分化而来的冻存小胶质细胞

产品优势

  • 使用源自经过高度表征的hiPSC对照系的即用型冻存人小胶质细胞，节省时间
  • 获得高纯度、处于非激活状态的功能性小胶质细胞
  • 可使用STEMdiff™小胶质细胞成熟试剂盒维持培养长达20天
  • 通过使用相同遗传背景生成的神经元-胶质细胞共培养物，确保生理相关性
  • 灵活地构建神经发育、疾病、药物筛选、毒理学和细胞治疗应用模型
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  1. STEMdiff™ 小胶质细胞成熟试剂盒
    STEMdiff™ 小胶质细胞成熟试剂盒
  2. DMEM/F-12，含15 mM HEPES
    DMEM/F-12，含15 mM HEPES
  3. Y-27632（二盐酸盐）
    Y-27632（二盐酸盐）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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概览

使用高质量、即用型的冻存人诱导多能干细胞（iPSC）衍生的小胶质细胞，助您自信地加速神经科学研究。在STEMdiff™小胶质细胞成熟试剂盒（产品号 #100-0020）中复苏后，即可立即用于下游应用。这些小胶质细胞具有功能性，处于非活化状态，并且具有高纯度（>70% CD45+/CD11b+、P2RY12+和TREM2+）。人iPSC衍生的小胶质细胞是在完全无血清的系统中，由经过高度表征的人iPSC对照系SCTi003-A（产品号 #200-0511）制备而来。这些胶质细胞在复苏后可维持培养长达20天，是用于研究神经炎症通路、模拟人神经发育和疾病、共培养应用、药物筛选、毒性检测和细胞疗法验证的多功能工具。

本产品仅供研究使用（RUO），已获准用于学术和商业用途。血液样本的采集符合伦理规范，并已获得机构审查委员会（IRB）或其他监管机构批准的知情同意书和方案。有关供体详细信息和源细胞库的细胞质量鉴定，请参阅下方的数据图表部分。有关更多详细信息，请参阅批次特异性的分析证书以及有关iPSC系的常见问题解答。

 

分类
冻存

细胞类型
上皮细胞

应用
PSC衍生造血细胞，小胶质细胞，PSC衍生神经细胞

物种
人

细胞与组织来源
多能干细胞

应用方向
细胞培养，功能检测，细胞毒性检测

研究领域
疾病建模，免疫学，神经科学，干细胞生物学

 

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实验数据

Human iPSC-Derived Microglia morphology

Figure 1. Human iPSC-Derived Microglia Exhibit High-Quality Morphology

Cryopreserved Human iPSC-Derived Microglia generated from SCTi003-A iPSCs were thawed and plated onto Matrigel®-coated plates at 1.1 x 10^4 cells/cm² in STEMdiff™ Microglia Maturation Kit. Microglia were incubated at 37°C and subsequently imaged on Days 2, 10 (before replating), and 20 (after replating) by brightfield microscopy at 10X magnification. Microglia display a compact morphology with moderate ramification due to reduced adherence, as expected in a healthy homeostatic microglia monoculture. iPSC = induced pluripotent stem cell.

Human iPSC-Derived Microglia Exhibit High Purity and Express Characteristic Glial Markers

Figure 2. Human iPSC-Derived Microglia Exhibit High Purity and Express Characteristic Glial Markers

Cryopreserved Human iPSC-Derived Microglia generated from SCTi003-A iPSCs were thawed and cultured using STEMdiff™ Microglia Maturation Kit. Microglia co-expressed CD45 and CD11b and expressed P2RY12 and TREM2 markers, as measured by flow cytometry on Days 0 and 10 post-thaw. The bars show the mean ± standard deviation. Technical replicates (n = 1 - 2) were averaged, and each dot in the graph represents an experimental replicate with different manufacturing lots separated by color. iPSC = induced pluripotent stem cell.

Human iPSC-Derived Microglia Display Cytokine Release in Response to Inflammatory Stimuli

Figure 3. Human iPSC-Derived Microglia Display Cytokine Release in Response to Inflammatory Stimuli

Cryopreserved Human iPSC-Derived Microglia generated from SCTi003-A iPSCs were thawed and plated at 100,000 cells per well in a 24-well plate using STEMdiff™ Microglia Maturation Kit for 1 day. Microglia were treated with water (control) or stimulated with 100 ng/mL LPS for 24 hours. (A) Brightfield microscopy shows the expected microglia morphology, including small cell bodies and ramified processes, under vehicle control conditions. After LPS treatment, activated microglial morphology with larger cell bodies, hypertrophic ramification, ameboid-like or rod-like shapes is observed. (B) The release of pro-inflammatory cytokines (TNFα and IL-6) was measured by Duoset ELISA with 20X dilution of the LPS-treated supernatants and the undiluted control supernatants. Microglia release cytokines in response to LPS treatment, as expected. Technical replicates (n = 2) were averaged, and each dot in the graph represents an experimental replicate with different manufacturing lots separated by color.*** p ≤ 0.001 and **** p ≤ 0.0001. LPS = lipopolysaccharide; iPSC = induced pluripotent stem cell.

Human iPSC-Derived Microglia Are Capable of Phagocytosis

Figure 4. Human iPSC-Derived Microglia Are Capable of Phagocytosis

Cryopreserved Human iPSC-Derived Microglia generated from SCTi003-A iPSCs were thawed and cultured using STEMdiff™ Microglia Maturation Kit for 1 day. Phagocytosis was assessed by adding pH-sensitive bioindicator particles (25 µg/mL), and particle uptake was tracked over 24 hours using live-cell imaging. As the particles are phagocytosed, the particles turn red and are concentrated within the cells. Over time, the microglia display an activated ameboid morphology. iPSC = induced pluripotent stem cell.

Human iPSC-Derived Microglia Can Be Tri-Cultured with Neurons and Astrocytes to Model Cell-Cell Interactions In Vitro

Figure 5. Human iPSC-Derived Microglia Can Be Tri-Cultured with Neurons and Astrocytes to Model Cell-Cell Interactions In Vitro

Representative immunocytochemistry images display Human iPSC-Derived Microglia tri-cultured with Human iPSC-Derived Astrocytes and Human iPSC-Derived Forebrain Neurons for 7 or 20 days using BrainPhys™ hPSC Neuron Kit supplemented with STEMdiff™ Microglia Supplement 2 (Component #100-0023 of STEMdiff™ Microglia Differentiation Kit). Cells were stained for MAP2 (cyan, neuronal dendrite marker), IBA1 (pink, microglia marker), GFAP (green, astrocyte marker), and DAPI (grey, nuclei). The presence of MAP2-positive neurons, GFAP-positive astrocytes, and IBA1-positive microglia confirms the successful establishment of tri-culture conditions. iPSC = induced pluripotent stem cell.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Microglia
Catalog #
200-1100, 200-1101
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages
Workflow Stages for

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物种 人类

法律声明：

These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details.

 

质量声明：

除非另有说明，否则产品仅供研究使用，不得用于人或动物的诊断或治疗用途。

 

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