人iPSC衍生的肝脏类器官

由人诱导多能干细胞系生成的冻存人肝细胞样细胞衍生类器官

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由人诱导多能干细胞系生成的冻存人肝细胞样细胞衍生类器官

产品优势

使用预先生成、冻存、源自特征明确的hiPSC对照系的肝脏类器官，从而节省时间
使用可立即进行终末分化的肝脏类器官，简化您的工作流程
使用STEMdiff™肝脏类器官生长培养基，在解冻后扩增类器官，高效扩大培养规模
使用表达关键成熟肝脏特异性标志物并在分化后表现出重要肝功能的肝脏类器官，获得具有生理相关性的实验结果
在生理相关性的3D系统中模拟肝脏生物学、评估药物引起的肝损伤并评估人特异性的代谢谱

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总览

使用高质量、冻存的人诱导多能干细胞（iPSC）来源的肝脏类器官，自信地推进您的肝脏研究。这些三维体外类器官模型源自特征明确的健康对照iPSC细胞系，为研究肝脏发育、药物性肝损伤（DILI）、肝毒性和代谢提供了一个与人体生理性相关的系统。人iPSC来源的肝脏类器官使用 STEMdiff™肝细胞试剂盒（产品号 #100-0520）生成，并在 STEMdiff™肝脏类器官培养基（产品号 #100-1773）中培养。解冻后，这些肝类器官可以进行多次传代培养。后续成熟培养可使用STEMdiff™肝类器官分化培养基（产品号 #100-1774），以获得表达成熟肝细胞标志物（如ALB、CYP3A4、ASGR1和CPS1）并展现关键功能特征（包括白蛋白分泌和CYP3A4活性）的肝类器官。

本产品仅供研究使用 (RUO)，已获得学术和商业用途的许可。原始供体血液样本的获取符合伦理规范，并已获得机构审查委员会（IRB）或其他监管机构批准的知情同意书和方案。更多详情请参阅批次特定的分析证书以及关于iPSC系列产品的常见问题解答。

某些产品仅在特定地区有售。请联系您的销售代表或技术支持部门了解更多信息。

实验数据

Figure 1. Workflow for Recovery, Expansion, and Differentiation of Human iPSC-Derived Hepatic Organoids

(A) Human iPSC-derived hepatic organoid fragments can be thawed, seeded in Matrigel® domes, and cultured in STEMdiff™ Hepatic Organoid Growth Medium (Catalog #100-1773) to generate confluent cultures within 7 days. Organoids can then be expanded every 6 - 10 days via fragment passaging in STEMdiff™ Hepatic Organoid Growth Medium. (B) After at least one passage post-thaw, cultures can be switched to STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) to differentiate organoids toward more mature hepatic cell types. iPSC = induced pluripotent stem cell

Figure 2. Human iPSC-Derived Hepatic Organoids Exhibit Efficient Post-Thaw Recovery and Expansion

Human iPSC-Derived Hepatic Organoids demonstrate robust (A) post-thaw recovery of organoid fragments (N = 11 experiments) and (B) efficient expansion across 9 passages. Human iPSC-Derived Hepatic Organoids derived from SCTi003-A were thawed and expanded in STEMdiff™ Organoid Growth Medium (Catalog #100-1773), and cumulative fragment yields were quantified over 9 passages (N = 5 experiments). Error bars = SD. iPSC = induced pluripotent stem cell

Figure 3. Human iPSC-Derived Hepatic Organoids Can Be Differentiated for Use in Downstream Assays

Representative images of Human iPSC-Derived Hepatic Organoids (A) thawed and cultured in STEMdiff™ Hepatic Organoid Growth Medium (OGM; Catalog #100-1773) for 7 days, and (B) following 10 days of differentiation using STEMdiff™ Hepatic Organoid Differentiation Medium (ODM; Catalog #100-1774). iPSC = induced pluripotent stem cell

Figure 4. Differentiated Human iPSC-Derived Hepatic Organoids Express Key Mature Hepatocyte Markers

Representative immunofluorescence images of Human iPSC-Derived Hepatic Organoids derived from SCTi003-A that were differentiated using STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) for 10 days. Differentiated hepatic organoids express hepatic transcription factor (A, B) HNF4A, and mature hepatocyte markers (A) ALB, (B, C, D) A1AT, and (C) BSEP. Ductal marker (D) KRT19 and epithelial marker (B) ECAD are also expressed, indicating a hepatobiliary phenotype. Nuclei are counterstained with DAPI. iPSC = induced pluripotent stem cell; ALB = albumin; A1AT = alpha-1 antitrypsin; BSEP = bile salt export pump; HNF4A = hepatocyte nuclear factor 4 alpha; KRT19 = cytokeratin 19; ECAD = e-cadherin

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

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Human iPSC-Derived Hepatic Organoids

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