EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #19849_C
免疫磁珠负选细胞分选试剂盒
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总览
使用 EasySep™ 小鼠/人嵌合体细胞分离试剂盒，可通过免疫磁性负选法从人异种移植受体小鼠的骨髓、脾脏或外周血样本中轻松高效地分离高纯度的人细胞。EasySep™ 技术在已发表研究中被广泛应用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。
在此 EasySep™ 负选步骤中，不需要的细胞通过针对小鼠造血细胞的生物素化抗体和包被链霉亲和素的磁性颗粒进行标记。经 EasySep™ 磁体分离后，带有磁性标记的细胞被去除，未标记的目标细胞通过倾倒或移液方式转移至新试管中。磁性细胞分离完成后，目标细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。
了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。
磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyPlate™ EasySep™磁极（产品号 #18102）
• EasyEights™ EasySep™磁极（产品号 #18103）
分类
细胞分选试剂盒
种属
小鼠
样本来源
骨髓、脾脏、全血
分选方法
负选
应用
细胞分选
品牌
EasySep
研究领域
癌症，免疫
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Resources and Publications
Educational Materials (10)
Frequently Asked Questions
Publications (2)
Despite mutation acquisition in hematopoietic stem cells, JMML-propagating cells are not always restricted to this compartment. Leukemia 2020 jun
Abstract
Juvenile myelomonocytic leukemia (JMML) is a rare aggressive myelodysplastic/myeloproliferative neoplasm of early childhood, initiated by RAS-activating mutations. Genomic analyses have recently described JMML mutational landscape; however, the nature of JMML-propagating cells (JMML-PCs) and the clonal architecture of the disease remained until now elusive. Combining genomic (exome, RNA-seq), Colony forming assay and xenograft studies, we detect the presence of JMML-PCs that faithfully reproduce JMML features including the complex/nonlinear organization of dominant/minor clones, both at diagnosis and relapse. Further integrated analysis also reveals that although the mutations are acquired in hematopoietic stem cells, JMML-PCs are not always restricted to this compartment, highlighting the heterogeneity of the disease during the initiation steps. We show that the hematopoietic stem/progenitor cell phenotype is globally maintained in JMML despite overexpression of CD90/THY-1 in a subset of patients. This study shed new lights into the ontogeny of JMML, and the identity of JMML-PCs, and provides robust models to monitor the disease and test novel therapeutic approaches.
Genetically Engineered Cell-Derived Nanoparticles for Targeted Breast Cancer Immunotherapy. Molecular therapy : the journal of the American Society of Gene Therapy 2019 nov
Abstract
Exosomes are nanosized membranous vesicles secreted by a variety of cells. Due to their unique and pharmacologically important properties, cell-derived exosome nanoparticles have drawn significant interest for drug development. By genetically modifying exosomes with two distinct types of surface-displayed monoclonal antibodies, we have developed an exosome platform termed synthetic multivalent antibodies retargeted exosome (SMART-Exo) for controlling cellular immunity. Here, we apply this approach to human epidermal growth factor receptor 2 (HER2)-expressing breast cancer by engineering exosomes through genetic display of both anti-human CD3 and anti-human HER2 antibodies, resulting in SMART-Exos dually targeting T cell CD3 and breast cancer-associated HER2 receptors. By redirecting and activating cytotoxic T cells toward attacking HER2-expressing breast cancer cells, the designed SMART-Exos exhibited highly potent and specific anti-tumor activity both in vitro and in vivo. This work demonstrates preclinical feasibility of utilizing endogenous exosomes for targeted breast cancer immunotherapy and the SMART-Exos as a broadly applicable platform technology for the development of next-generation immuno-nanomedicines.
更多信息
| 物种 | 小鼠 |
|---|---|
| Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102) |
| 样本来源 | 全血, 脾脏, 骨髓 |
| Selection Method | Negative |
标记抗体
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抗小鼠CD45R抗体，clone RA3-6B2大鼠Monoclonal IgG2a抗体，抗人、小鼠、猫CD45R（B220）
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抗小鼠TER119抗体，clone TER-119抗小鼠TER119的大鼠（WI）Monoclonal IgG2b抗体
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抗人CD45抗体，clone HI30小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45
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